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European Journal of Plant Pathology

, Volume 125, Issue 2, pp 235–244 | Cite as

Identification and molecular mapping of the rice bacterial blight resistance gene allelic to Xa7 from an elite restorer line Zhenhui 084

  • Yuchen Zhang
  • Jianfei Wang
  • Jianwei Pan
  • Zhimin Gu
  • Xifeng Chen
  • Yang Jin
  • Feng Liu
  • Hongsheng Zhang
  • Bojun Ma
Article

Abstract

Rice bacterial blight (BB), caused by Xanthomonas oryzae pv. Oryzae (Xoo), is a serious disease in rice production worldwide. Rice cv. Zhenhui 084, a newly developed strong indica restorer line, exhibits high resistance to most of the Philippine races of BB and has been widely used in rice hybrids in China; however, the resistance gene has not yet been cloned. Here, we show that the resistance of Zhenhui 084 to Xoo strains is similar to that of IRBB7 containing Xa7, a durable and broad resistance dominant gene for BB. To map the resistance gene in Zhenhui 084, a F2 population with 331 highly susceptible individuals derived from a cross between Chenghui 448 and Zhenhui 084 was built. We finely mapped the target R gene to a region between two proximal markers RM20576 and MY4 in rice chromosome 6. A marker-based physical map of chromosome six was used to construct the contig covering the genomic region between two markers RM20576 and MY4. The target gene was assumed to be in an interval of approximate 200 kb, in which 16 candidate genes were predicted. Our findings will greatly facilitate the isolation and characterisation of the target R gene allelic to Xa7. Additionally, two PCR-based markers, tightly linked to the target R gene locus, will be a useful tool for the marker-assisted selection of the target R gene allelic to Xa7 in breeding programmes.

Keywords

Bacterial blight Identification Molecular mapping Rice Xa7 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grants No. 30771329, 30800677), the Zhejiang Normal University Innovative Research Team Programme, National Key Programmes for Transgenic Crops (2008ZX08009-003-001), and the Ph.D. Programme Foundation from the Ministry of Education (No. 20060307035) and the Programme for Changjiang Scholars and the Innovative Research Team in the University (PCISRT).

References

  1. Babujee, L., & Gnanamickam, S. S. (2000). Molecular tools for characterization of rice blast pathogen (Magnaporthe grisea) population and molecular marker-assisted breeding for disease resistance. Current Science, 78, 248–257.Google Scholar
  2. Banerjee, D., Zhang, X., & Bent, A. F. (2001). The leucine-rich repeat domain can determine effective interaction between RPS2 and other host factors in Arabidopsis RPS2-mediated disease resistance. Genetics, 158, 439–450.PubMedGoogle Scholar
  3. Bonas, U., Stall, R. E., & Staskawicz, B. (1989). Genetic and structural characterization of the avirulence gene avrBs3 from Xanthomonas campestris pv. vesicatoria. Molecular Genetics and Genomics, 218, 127–136.Google Scholar
  4. Cheema, K. K., Grewal, N. K., Vikal, Y., Sharma, R., Lore, J. S., Das, A. et al. (2008). A novel bacterial blight resistance gene from Oryza nivara mapped to 38 kb region on chromosome 4L and transferred to Oryza sativa L. Genetics Research, 90, 397–407.PubMedCrossRefGoogle Scholar
  5. Chen, H., Wang, S., & Zhang, Q. (2002). A new gene for bacterial blight resistance in rice located on chromosome 12 identified from Minghui 63, and elite restorer line. Phytopathology, 92, 750–754. doi: 10.1094/PHYTO.2002.92.7.750.PubMedCrossRefGoogle Scholar
  6. Chen, S., Huang, Z. H., Zeng, L. X., Yang, J. Y., Liu, Q. G., & Zhu, X. Y. (2008). High-resolution mapping and gene prediction of Xanthomonas oryzae pv. oryzae resistance gene Xa7. Molecular Breeding, 22, 433–441. doi: 10.1007/s11032-008-9187-1.CrossRefGoogle Scholar
  7. Chu, Z. H., Yuan, M., Yao, J. L., Ge, X. J., Yuan, B., Xu, C. G., et al. (2006). Promoter mutations of an essential gene for pollen development result in disease resistance in rice. Genes & Development, 20, 1250–1255. doi: 10.1101/gad.1416306.CrossRefGoogle Scholar
  8. Ezuka, A., & Sakaguchi, S. (1978). Host-parasite relationship in bacterial blight of rice caused by Xanthomonas oryzae pv. oryzae. Review of Plant Protection Research, 11, 93–118.Google Scholar
  9. Gu, K. Y., Yang, B., Tian, D. S., Wu, L. F., Wang, D. J., Sreekala, C., et al. (2005). R gene expression induced by a type-III effector triggers disease resistance in rice. Nature, 435, 1122–1125. doi: 10.1038/nature03630.PubMedCrossRefGoogle Scholar
  10. Henrissat, B. (1998). Glycosidase families. Biochemical Society Transactions, 26, 153–156.PubMedGoogle Scholar
  11. Hopkins, C. M., White, F. F., Choi, S. H., Guo, A., & Leach, J. E. (1992). Identification of a family of avirulence genes from Xanthomonas oryzae pv. oryzae. Molecular Plant-Microbe Interactions, 5, 451–459.PubMedGoogle Scholar
  12. Huang, N., Angeles, E. R., Domingo, J., Magpantay, G., Singh, S., Zhang, G., et al. (1997). Pyramiding bacterial blight resistance genes in rice: Marker assisted selection using RFLP and PCR. Theoretical and Applied Genetics, 95, 313–320. doi: 10.1007/s001220050565.CrossRefGoogle Scholar
  13. IRGSP (2005). The map-based sequence of the rice genome. Nature, 436, 793–800.Google Scholar
  14. Iyer, A. S., & McCouch, S. R. (2004). The rice bacterial blight resistance gene xa5 encodes a novel form of disease resistance. Molecular Plant-Microbe Interactions, 17, 1348–1354. doi: 10.1094/MPMI.2004.17.12.1348.PubMedCrossRefGoogle Scholar
  15. Jiang, G. H., Xia, Z. H., Zhou, Y. L., Wan, J., Li, D. Y., Chen, R. S., et al. (2006). Testifying the rice bacterial blight resistance gene xa5 by genetic complementation and further analyzing xa5 (Xa5) in comparison with its homolog TFIIAc1. Molecular Genetics and Genomics, 275, 354–366. doi: 10.1007/s00438-005-0091-7.PubMedCrossRefGoogle Scholar
  16. Kauffman, H. E., Reddy, A. P. K., Hsieh, S. P. Y., & Merca, S. D. (1973). An improved technique for evaluating resistance of rice varieties to Xanthomonas oryzae pv. oryzae. Plant Disease Report, 57, 537–541.Google Scholar
  17. Kehres, D. G., Zaharik, M. L., Finlay, B. B., & Maguire, M. E. (2000). The NRAMP proteins of Salmonella typhimurium and Escherichia coli are selective manganese transporters involved in the response to reactive oxygen. Molecular Microbiology, 36, 1085–1100. doi: 10.1046/j.1365-2958.2000.01922.x.PubMedCrossRefGoogle Scholar
  18. Khush, G. S., & Angeles, E. R. (1999). A new gene for resistance to race 6 of bacterial blight in rice, Oryza sativa L. Rice Genetics Newsletter, 16, 92–93.Google Scholar
  19. Larsen, C. N., & Wang, H. (2002). The ubiquitin superfamily: members, features, and phylogenies. Journal of Proteome Research, 5, 411–419. doi: 10.1021/pr025522n.CrossRefGoogle Scholar
  20. Lee, K. S., Rasabandith, S., Angeles, E. R., & Khush, G. S. (2003). Inheritance of resistance to bacterial blight in 21 cultivars of rice. Phytopathology, 93, 147–152. doi: 10.1094/PHYTO.2003.93.2.147.PubMedCrossRefGoogle Scholar
  21. Leach, J. E., & White, F. F. (1996). Bacterial avirulence genes. Annual review of Phytopathology, 34, 153–179. doi: 10.1146/annurev.phyto.34.1.153.PubMedCrossRefGoogle Scholar
  22. Lin, X. H., Zhang, D. P., Xie, Y. F., Gao, H. P., & Zhang, Q. (1996). Identifying and mapping a new gene for bacterial blight resistance in rice based on RFLP markers. Phytopathology, 86, 1156–1193. doi: 10.1094/Phyto-86-1156.CrossRefGoogle Scholar
  23. Marchler-Bauer, A., Anderson, J. B., Derbyshire, M. K., DeWeese-Scott, C., Gonzales, N. R., Gwadz, M., et al. (2007). CDD: a conserved domain database for interactive domain family analysis. Nucleic Acids Research, 35, D237–D240. doi: 10.1093/nar/gkl951.PubMedCrossRefGoogle Scholar
  24. Mew, T. W., & Vera Cruz, C. M. (1979). Variability of Xanthomonas oryzae pv. oryzae: Specificity in infection of rice differentials. Phytopathology, 69, 152–155. doi: 10.1094/Phyto-69-152.CrossRefGoogle Scholar
  25. Nagato, Y., & Yoshimura, A. (1998). Report of the committee on gene symbolization, nomenclature and linkage groups. Rice Genetics Newsletter, 15, 13–74.Google Scholar
  26. Nelson, N. (1999). Metal ion transporters and homeostasis. EMBO Journal, 18, 4361–4371. doi: 10.1093/emboj/18.16.4361.PubMedCrossRefGoogle Scholar
  27. Ogawa, T., Yamamoto, T., Khush, G. S., & Mew, T. W. (1991). Breeding of near-isogenic lines of rice with single genes for resistance to bacterial blight pathogen (Xanthomonas oryzae pv. oryzae). Japanese Journal of Breeding, 41, 523–529.Google Scholar
  28. Porter, B. W., Chittoor, J. M., Yano, M., Sasaki, T., & White, F. F. (2003). Development and mapping linked to the rice bacterial blight resistance gene Xa7. Crop Science, 43, 1484–1492.CrossRefGoogle Scholar
  29. Sablowski, R. W., & Meyerowitz, E. M. (1998). A homolog of NO APICAL MERISTEM is an immediate target of the floral homeotic genes APETALA3/PISTILLATA. Cell, 92, 93–103. doi: 10.1016/S0092-8674(00)80902-2.PubMedCrossRefGoogle Scholar
  30. Sheng, S. L., Gong, H. B., Diao, L. P., Hu, C. M., Lin, T. Z., & Zhou, Y. W. (2002). Breeding and utilization of the indica rice restorer line Zhenhui 084. Hybrid Rice, 17, 627.Google Scholar
  31. Sidhu, G. S., Khush, G. S., & Mew, T. W. (1978). Genetic analysis of bacterial blight resistance in seventy-four cultivars of rice, Oryza sativa L. Theoretical and Applied Genetics, 53, 105–111. doi: 10.1007/BF00272687.CrossRefGoogle Scholar
  32. Singh, K., Vikal, Y., Mahajan, R., Cheema, K. K., Bhatia, D., Sharma, R., et al. (2007, October). Three novel bacterial blight resistance genes identified, mapped and transferred to cultivated rice O. sativa L. (Paper presented at the 2nd International Conference on Bacterial Blight of Rice, Nanjing).Google Scholar
  33. Song, W. Y., Wang, G. L., Chen, L. L., Kim, H. S., Pi, L. Y., Holsten, T., et al. (1995). A receptor kinase-like protein encoded by the rice disease resistance gene, Xa21. Science, 270, 1804–1806. doi: 10.1126/science.270.5243.1804.PubMedCrossRefGoogle Scholar
  34. Souer, E., Van Houwelingen, A., Kloos, D., Mol, J. N. M., & Koes, R. E. (1996). The no apical meristem gene of Petunia is required for pattern formation in embryos and flowers and is expressed at meristem and primordia boundaries. Cell, 85, 159–170. doi: 10.1016/S0092-8674(00)81093-4.PubMedCrossRefGoogle Scholar
  35. Sun, X. L., Cao, Y. L., Yang, Z. F., Xu, C. G., Li, X. H., Wang, S. P., et al. (2004). Xa26, a gene conferring resistance to Xanthomonas oryzae pv. oryzae in rice, encodes an LRR receptor kinase-like protein. The Plant Journal, 7, 17–527.Google Scholar
  36. Tan, G. X., Ren, X., Weng, Q. M., Shi, Z. Y., Zhu, L. L., & He, G. C. (2004). Mapping of a new resistance gene to bacterial blight in rice line introgressed from Oryza officinalis. Acta Genetica Sinica, 31, 724–729.PubMedGoogle Scholar
  37. Vera Cruz, C. M., Bai, J. F., Ona, I., Leung, H., Nelson, R. J., Mew, T. W., et al. (2000). Predicting durability of a disease resistance gene based on an assessment of the fitness loss and epidemiological consequences of avirulence gene mutation. Proceedings of the National Academy of Sciences of the United States of America, 97, 13500–13505. doi: 10.1073/pnas.250271997.PubMedCrossRefGoogle Scholar
  38. White, F. F., Yang, B., & Johnson, L. B. (2000). Prospects for understanding avirulence gene function. Current Opinion in Plant Biology, 3, 291–298. doi: 10.1016/S1369-5266(00)00082-0.PubMedCrossRefGoogle Scholar
  39. Xiang, Y., Cao, Y. L., Xu, C. Q., Li, X. H., & Wang, S. P. (2006). Xa3, conferring resistance for rice bacterial blight and encoding a receptor kinase-like protein, is the same as Xa26. Theoretical and Applied Genetics, 113, 1347–1355. doi: 10.1007/s00122-006-0388-x.PubMedCrossRefGoogle Scholar
  40. Yang, B., Zhu, W., Johnson, L. B., & White, F. F. (2000). The virulence factor AvrXa7 of Xanthomonas oryzae pv. oryzae is a type III secretion pathway-dependent nuclear-localized double-stranded DNA-binding protein. Proceedings of the National Academy of Sciences of the United States of America, 97, 9807–9812. doi: 10.1073/pnas.170286897.PubMedCrossRefGoogle Scholar
  41. Yoshimura, S., Yamanouchi, U., Katayose, Y., Toki, S., Wang, Z. X., Kono, I., et al. (1998). Expression of Xa1, a bacterial blight resistance gene in rice, is induced by bacterial inoculation. Proceedings of the National Academy of Sciences of the United States of America, 95, 1663–1668. doi: 10.1073/pnas.95.4.1663.PubMedCrossRefGoogle Scholar
  42. Zhang, Q., Lin, S. C., Zhao, B. Y., Wang, C. L., Yang, W. C., Zhou, Y. L., et al. (1998). Identification and tagging a new gene for resistance to bacterial blight (Xanthomonas oryzae pv. oryzae) from O. rufipogon. Rice Genetics Newsletter, 15, 138–142.Google Scholar

Copyright information

© KNPV 2009

Authors and Affiliations

  • Yuchen Zhang
    • 1
  • Jianfei Wang
    • 2
  • Jianwei Pan
    • 1
  • Zhimin Gu
    • 1
  • Xifeng Chen
    • 1
  • Yang Jin
    • 1
  • Feng Liu
    • 1
  • Hongsheng Zhang
    • 2
  • Bojun Ma
    • 1
  1. 1.College of Chemistry and Life SciencesZhejiang Normal UniversityJinhuaPeople’s Republic of China
  2. 2.State Key Laboratory of Crop Genetics and Germplasm EnhancementNanjing Agricultural UniversityNanjingPeople’s Republic of China

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