European Journal of Plant Pathology

, Volume 125, Issue 1, pp 109–118 | Cite as

Teratosphaeria (Mycosphaerella) nubilosa, the causal agent of Mycosphaerella leaf disease (MLD), recently introduced into Uruguay

  • Guillermo PérezEmail author
  • Gavin C. Hunter
  • Bernard Slippers
  • Carlos Pérez
  • Brenda D. Wingfield
  • Michael J. Wingfield


Mycosphaerella leaf disease on Eucalyptus is well known in Uruguay but none of the more serious Mycosphaerella spp. and Teratosphaeria spp. causing this disease have yet been found. In the autumn of 2007, more severe defoliation than has been known in the past and associated with symptoms resembling Mycosphaerella infections was observed on Eucalyptus globulus. Isolations and identifications based on morphology and DNA sequence comparisons showed that the causal agent of the defoliation is the well known and serious pathogen Teratosphaeria nubilosa (=Mycosphaerella nubilosa). This is the first record of the pathogen in South America. Using ten microsatellite loci previously developed for T. nubilosa, only one multilocus haplotype was found from 46 T. nubilosa collected isolates. Interestingly, this haplotype was the same as one previously found in Portugal and Spain. The results suggest that T. nubilosa has recently been introduced into Uruguay and that it most likely originated from the Iberian Peninsula where E. globulus is widely planted.


Eucalyptus globulus Forest pathology Genetic population Introduced pathogen Microsatellite markers Mycosphaerella nubilosa 



Mycosphaerella leaf disease



We thank the National Research Foundation (NRF), members of the Tree Protection Co-operative Programme (TPCP) and the Department of Science and Technology/ NRF Centre of Excellence in Tree Health Biotechnology (CTHB), South Africa, for financial support.


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Copyright information

© KNPV 2009

Authors and Affiliations

  • Guillermo Pérez
    • 1
    Email author
  • Gavin C. Hunter
    • 1
    • 2
  • Bernard Slippers
    • 3
  • Carlos Pérez
    • 4
  • Brenda D. Wingfield
    • 3
  • Michael J. Wingfield
    • 1
  1. 1.Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute (FABI)University of PretoriaPretoriaSouth Africa
  2. 2.CBS, Fungal Biodiversity CentreUtrechtNetherlands
  3. 3.Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI)University of PretoriaPretoriaSouth Africa
  4. 4.Departamento de Protección Vegetal, Facultad de AgronomíaUniversidad de la RepúblicaPaysandúUruguay

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