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Diverse Fusarium solani isolates colonise agricultural environments in Ethiopia

  • M. BogaleEmail author
  • E.T. Steenkamp
  • M.J. Wingfield
  • B.D. Wingfield
Article

Abstract

Fusarium solani is a fungal pathogen that infects many different genera of plants. It represents one of the two Fusarium spp. commonly isolated from agricultural soils and plant tissues in Ethiopia. To determine the diversity of F. solani in Ethiopia, we studied 43 isolates using Amplified Fragment Length Polymorphism (AFLP) and nucleotide sequences of the Translation Elongation Factor 1α (TEF-1α) and β-tubulin genes. TEF-1α sequences from GenBank, representing previously described species and clades of the F. solani-Haematonectria haematococca complex, were also included for comparative purposes. Phylogenetic analyses of the TEF-1α data separated the isolates into three groups corresponding with the three previously described clades (Clades 1–3) for this fungus. The Ethiopian isolates aggregated into one group corresponding to Clade 3. TEF-1α, β-tubulin and AFLPs further separated the Ethiopian isolates into a number of clusters and apparently novel phylogenetic lineages. Although the biological and ecological significance of these lineages and clusters is unclear, our data show that the Ethiopian agricultural environment is rich in species and lineages of the F. solani-H. haematococca complex.

Keywords

AFLP β-tubulin Ethiopia Fusarium solani TEF-1α 

Notes

Acknowledgements

This study was financially supported by the Tree Protection Co-operative Programme (TPCP) of FABI, University of Pretoria, South Africa; the National Research Foundation (NRF), South Africa; and the Agricultural Research and Training Programme (ARTP) of the Ethiopian Agricultural Research Organization (EARO), Ethiopia. We thank Dr. Kerry O’Donnell of the United States Department of Agriculture (USDA) for kindly supplying some of the TEF-1α sequence information used in this study.

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Copyright information

© KNPV 2009

Authors and Affiliations

  • M. Bogale
    • 1
    Email author
  • E.T. Steenkamp
    • 2
  • M.J. Wingfield
    • 2
  • B.D. Wingfield
    • 1
  1. 1.Department of Genetics, Forestry and Agricultural Biotechnology InstituteUniversity of PretoriaPretoriaSouth Africa
  2. 2.Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology InstituteUniversity of PretoriaPretoriaSouth Africa

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