European Journal of Plant Pathology

, Volume 124, Issue 2, pp 303–308 | Cite as

Identification of phytotoxins from Botryosphaeria obtusa, a pathogen of black dead arm disease of grapevine

  • Jules Désiré Djoukeng
  • Suzanna Polli
  • Philippe Larignon
  • Eliane Abou-Mansour


A bioassay-guided fractionation of a culture filtrate of Botryosphaeria obtusa led to the isolation of four dihydroisocoumarins, named mellein 1, 4-hydroxymellein 2, 7-hydroxymellein 3 and the new 4,7-dihydroxymellein 4. LC-UV-DAD-MS analysis of vine wood infected by B. obtusa revealed the presence of mellein (1). Botryosphaeria obtusa was also able to oxidise wood δ -resveratrol into the dimer delta-viniferin. The structures of isolated phytotoxins were established on the basis of IR, MS, 1D and 2D NMR.


Black dead arm Botryosphaeria obtusa Grapevine disease Isocoumarins Melleins Phytotoxins 



This project was funded by the National Centre of Competence in Research (NCCR) Plant Survival, a research programme of the Swiss Science Foundation, and supported by European Cooperation in the field of Scientific and Technical Research (COST) Action 858 and by a grant from the State Secretariat for Education and Research (Switzerland).


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Copyright information

© KNPV 2009

Authors and Affiliations

  • Jules Désiré Djoukeng
    • 1
  • Suzanna Polli
    • 1
  • Philippe Larignon
    • 2
  • Eliane Abou-Mansour
    • 1
    • 3
  1. 1.Institute of BiologyUniversity of NeuchâtelNeuchâtelSwitzerland
  2. 2.Institut Français de la Vigne et du Vin, Unité Rhône-MéditerranéeDomaine de DonadilleRodilhanFrance
  3. 3.Plant Biology DepartmentUniversity of FribourgFribourgSwitzerland

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