Host range and properties of Tomato chlorotic dwarf viroid
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We characterised the host range and physical properties of Tomato chlorotic dwarf viroid. Among the 46 plant species inoculated with the viroid, two in the family Compositae and 23 in the family Solanaceae were found to be systemic hosts. The viroids in the crude sap from diseased tomato plants were thermally inactivated by heating to 100°C for at least 40 min. These viroids also lost their infectivity when diluted in phosphate buffer to at least 10−6, or after 3 days of incubation at room temperature. However, the infectivity of the viroids in dried crude sap from the plants persisted throughout the 50-day test period.
KeywordsDisease Infection Pospiviroid TCDVd Thermal inactivation Tomato
We are grateful to S. Matsuura, the Hiroshima Prefectural Agriculture Research Centre, for helpful comments and discussion. This study was supported, in part, by a Grant-in-Aid from The Research Project for Utilizing Advanced Technologies in Agriculture, Forestry and Fisheries, administered by the Ministry of Agriculture, Forestry and Fisheries in Japan.
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