Molecular characterisation of Turnip mosaic virus isolates from Brassicaceae weeds
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Eight provinces of Iran were surveyed during 2003–2008 to find Brassicaceae reservoir weed hosts of Turnip mosaic virus (TuMV). A total of 532 weed samples were collected from plants with virus-like symptoms. The samples were tested for the presence of TuMV by enzyme-linked immunosorbent assay using specific antibodies. Among those tested, 340 samples (64%) were found to be infected with TuMV. Rapistrum rugosum, Sisymberium loeselii, S. irio and Hirschfeldia incana were identified as the Brassicaceae weed hosts of TuMV, and the former two plant species were found to be the most important weed hosts for the virus in Iran. The full-length sequences of the genomic RNAs of IRN TRa6 and IRN SS5 isolates from R. rugosum and S. loeselii were determined. No evidence of recombination was found in both isolates using different recombination-detecting programmes. Phylogenetic analyses of the weed isolates with representative isolates from the world showed that the IRN TRa6 and IRN SS5 isolates fell into an ancestral basal-Brassica group. This study shows for the first time the wide distribution and phylogenetic relationships of TuMV from weeds in the mid-Eurasia of Iran.
KeywordsTurnip mosaic virus Brassicaceae Weeds Iran Evolution Phylogeny
We are grateful to S. Sajedi, Botanic Department of Plant Pests and Diseases Research Institute (PPDRI), Iran, for identification of weed species, to Dr. S. Korkmaz, Department of Plant Protection, Faculty of Agriculture, University of Canakkale Onsekiz Mart, Turkey, for preparing some seeds used in the study, and to Prof. U. Melcher, Department of Biochemistry and Molecular Biology, Oklahoma State University, USA, for his kindly reading the manuscript. This work was supported by a grant from PPDRI of Iran and Grant-in-Aid for Scientific Research (B) No. 18405022 from the Japan Society for the Promotion of Science.
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