Molecular characterisation of Turnip mosaic virus isolates from Brassicaceae weeds
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Eight provinces of Iran were surveyed during 2003–2008 to find Brassicaceae reservoir weed hosts of Turnip mosaic virus (TuMV). A total of 532 weed samples were collected from plants with virus-like symptoms. The samples were tested for the presence of TuMV by enzyme-linked immunosorbent assay using specific antibodies. Among those tested, 340 samples (64%) were found to be infected with TuMV. Rapistrum rugosum, Sisymberium loeselii, S. irio and Hirschfeldia incana were identified as the Brassicaceae weed hosts of TuMV, and the former two plant species were found to be the most important weed hosts for the virus in Iran. The full-length sequences of the genomic RNAs of IRN TRa6 and IRN SS5 isolates from R. rugosum and S. loeselii were determined. No evidence of recombination was found in both isolates using different recombination-detecting programmes. Phylogenetic analyses of the weed isolates with representative isolates from the world showed that the IRN TRa6 and IRN SS5 isolates fell into an ancestral basal-Brassica group. This study shows for the first time the wide distribution and phylogenetic relationships of TuMV from weeds in the mid-Eurasia of Iran.
KeywordsTurnip mosaic virus Brassicaceae Weeds Iran Evolution Phylogeny
- Fauquet, C. M., Mayo, M. A., Maniloff, J., Desselberger, U., & Ball, L. A. (2005). Virus taxonomy: Classification and nomenclature of viruses, eighth report of the international committee on taxonomy of viruses. San Diego: Elsevier Academic.Google Scholar
- Felsenstein, J. (1993). PHYLIP (Phylogeny interference package), Version 3.5. Department of Genetics, University of Washington, Seattle.Google Scholar
- Hall, T. A. (1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98.Google Scholar
- Korkmaz, S., Tomitaka, Y., Onder, S., & Ohshima, K. (2008). Occurrence and molecular characterization of Turkish isolates of Turnip mosaic virus. Plant Pathology. doi:10.1111/j.1365-3059.2008.01902.x.Google Scholar
- Maynard Smith, J. (1992). Analyzing the mosaic structure of genes. Journal of Molecular Evolution, 34, 126–129.Google Scholar
- Ogawa, T., Tomitaka, Y., Nakagawa, A., & Ohshima, K. (2008). Genetic structure of a population of Potato virus Y inducing potato tuber necrotic ringspot disease in Japan; comparison with North American and European populations. Virus Research, 131, 199–212. doi:10.1016/j.virusres.2007.09.010.PubMedCrossRefGoogle Scholar
- Ohshima, K., Tomitaka, Y., Wood, J. T., Minematsu, Y., Kajiyama, H., Tomimura, K., et al. (2007). Patterns of recombination in Turnip mosaic virus genomic sequences indicate hotspots of recombination. The Journal of General Virology, 88, 298–315. doi:10.1099/vir.0.82335-0.PubMedCrossRefGoogle Scholar
- Provvidenti, R. (1996). Turnip mosaic potyvirus. In A. A. Brunt, K. Crabtree, M. J. Dallwitz, A. J. Gibbs, & L. Watson (Eds.), Viruses of plants (pp. 1340–1343). Wallingford: CAB International.Google Scholar
- Sawyer, S. A. (1999). GENECONV: A computer package for the statistical detection of gene conversion. Distributed by the author. Department of Mathematics, Washington University in St. Louis, available at http://www.math.wustl.edu/~sawyer.
- Strimmer, K., & von Haeseler, A. (1996). Quartet puzzling: a quartet maximum likelihood method for reconstructing tree topologies. Molecular Biology and Evolution, 13, 964–969.Google Scholar
- Strimmer, K., Goldman, N., & von Haeseler, A. (1997). Bayesian probabilities and quartet puzzling. Molecular Biology and Evolution, 14, 210–211.Google Scholar
- Tomimura, K., Gibbs, A. J., Jenner, C. E., Walsh, J. A., & Ohshima, K. (2003). The phylogeny of Turnip mosaic virus; comparisons of thirty-eight genomic sequences reveal a Eurasian origin and a recent ‘emergence’ in east Asia. Molecular Ecology, 12, 2099–2111. doi:10.1046/j.1365-294X.2003.01881.x.PubMedCrossRefGoogle Scholar