Pectobacterium carotovorum subsp. carotovorum can cause potato blackleg in temperate climates

  • Eisse G. de Haan
  • Toos C. E. M. Dekker-Nooren
  • Gé W. van den Bovenkamp
  • Arjen G. C. L. Speksnijder
  • Patricia S. van der Zouwen
  • Jan M. van der Wolf
Article

Abstract

It is well established that the pectinolytic bacteria Pectobacterium atrosepticum (Pca) and Dickeya spp. are causal organisms of blackleg in potato. In temperate climates, the role of Pectobacterium carotovorum subsp. carotovorum (Pcc) in potato blackleg, however, is unclear. In different western and central European countries plants are frequently found with blackleg from which only Pcc can be isolated, but not Pca or Dickeya spp. Nevertheless, tubers vacuum-infiltrated with Pcc strains have so far never yielded blackleg-diseased plants in field experiments in temperate climates. In this study, it is shown that potato tubers, vacuum-infiltrated with a subgroup of Pcc strains isolated in Europe, and planted in two different soil types, can result in up to 50% blackleg diseased plants.

Keywords

Erwinia carotovora subsp. carotovora Pathogenicity PCR-amplification Symptom expression Vacuum infiltration 

Abbreviations

Pca

Pectobacterium atrosepticum

Pcc

Pectobacterium carotovorum subsp. carotovorum

References

  1. Burkholder, W. H. (1953). Genus VI. 1917. In E. Winslow, et al. (Ed.), Bergey’s Manual of Determinative Bacteriology (7th ed., pp. 349–359). Baltimore, MD, USA: Williams and Wilkens.Google Scholar
  2. Clark, M. F., & Adams, A. N. (1977). Characteristics of the microplate method of enzyme-linked immunosorbent assay for detection of plant viruses. Journal of General Virology, 34, 475–483.PubMedCrossRefGoogle Scholar
  3. De Boer, S. H. (2002). Relative incidence of Erwinia carotovora subsp. atroseptica in stolon end and peridermal tissue of potato tubers in Canada. Plant Disease, 86, 960–964.CrossRefGoogle Scholar
  4. De Boer, S. H., & Kelman, A. (2001). Erwinia soft rot group. In N. W. Schaad, J. B. Jones, & W. Chun (Eds.), Laboratory Guide for Identification of Plant Pathogenic Bacteria ( 3rd ed., pp. 56–72). St. Paul MN: American Phytopathological Society.Google Scholar
  5. De Boer, S. H., Verdonck, L. H., Vruggink, P., Harju Bang, H. O., & De Ley, J. (1987). Serological and biochemical variation among potato strains of Erwinia carotovora subsp. atroseptica and their taxonomic relationship to other E. carotovora strains. Journal of Applied Bacteriology, 63, 487–495.Google Scholar
  6. De Boer, S. H., & Ward, L. J. (1995). PCR detection of Erwinia carotovora subsp. atroseptica associated with potato tissue. Phytopathology, 85, 854–858.CrossRefGoogle Scholar
  7. Dye, D. W. (1969). A taxonomic study of the genus Erwinia. II. The “Carotovora” group. New Zealand Journal of Science, 12, 81–97.Google Scholar
  8. Fraaije, B. A., Birbaum, Y., & Van den Bulk, R. W. (1996). Comparison of methods for detection of Erwinia carotovora spp. atroseptica in progeny tubers derived from inoculated tubers of Solanum tuberosum L. Journal of Phytopathology, 144, 551–557.CrossRefGoogle Scholar
  9. Gardan, L., Couy, C., Christen, R., & Galan, J. E. (2003). Evaluation of three subspecies of Pectobacterium carotovorum to species level: Pectobacterium atrosepticum sp. nov., Pectobacterium betavasculorum sp. nov. and Pectobacterium wasabiae sp. nov. International. Journal of Systematic and Evolutionary Microbiology, 53, 381–391.PubMedCrossRefGoogle Scholar
  10. GenStat (2007). GenStat for Windows. Release 9.1 (9th ed.). Oxford: VSN International Ltd.Google Scholar
  11. Helias, V., Andrivon, D., & Jouan, B. (2000). Development of symptoms caused by Erwinia carotovora spp. atroseptica under field conditions and their effects on the yield of individual potato plants. Plant Pathology, 49, 23–32.CrossRefGoogle Scholar
  12. Hyman, L. J., Toth, I. K., & Pérombelon, M. C. M. (1998). Isolation and identification. In Pages 60–65. In M. C. M. Pérombelon, & J. M. van der Wolf (Eds), Methods for the detection and quantification of Erwinia carotovora subsp. atroseptica on potatoes (pp. 60–65). Laboratory Manual Scottish Crop Research Institute. Invergowrie. Occasional Publication No. 10.Google Scholar
  13. Kang, H. W., Kwon, S. W., & Go, S. J. (2003). PCR-based specific and sensitive detection of Pectobacterium carotovorum spp. carotovorum by primers generated from URP-PCR fingerprinting-derived polymorphic band. Plant Pathology, 52, 127–133.CrossRefGoogle Scholar
  14. KNMI WWW klimaatdata (2007). Retrieved from http://www.knmi.nl/klimatologie/maand_en_seizoenoverzichten/maand/
  15. Lumb, V. M., Pérombelon, M. C. M., & Zutra, D. (1986). Studies of a wilt disease of the potato plant in Israel caused by Erwinia chrysanthemi. Plant Pathology, 35, 196–202.CrossRefGoogle Scholar
  16. McCullagh, P., & Nelder, J. A. (1989). Generalized Linear Models (2nd ed.). London: Chapman & Hall.Google Scholar
  17. Molina, J. J., & Harrison, D. D. (1977). The role of Erwinia carotovora in the epidemiology of potato blackleg. Relationship of Erwinia carotovora var. carotovora and Erwinia carotovora var. atroseptica to potato blackleg in Colorado. American Potato Journal, 54, 587–591.CrossRefGoogle Scholar
  18. Molina, J. J., & Harrison, D. D. (1980). The role of Erwinia carotovora in the epidemiology of potato blackleg. The effect of soil temperature on disease severity. American Potato Journal, 57, 351–363.CrossRefGoogle Scholar
  19. Nassar, A., Darasse, A., Lemattre, M., Kotoujansky, A., Dervin, C., Vedel, R., et al. (1996). Characterisation of Erwinia chrysanthemi by pectinolytic isozyme polymorphism and restriction fragment length polymorphism analysis of PCR-amplified fragments of pel genes. Applied and Environmental Microbiology, 62, 2228–2235.PubMedGoogle Scholar
  20. Peltzer, S., & Sivasithamparam, K. (1985). Soft–rot erwinias and stem rots in potatoes. Australian Journal of Experimental Agriculture, 25, 693–696.CrossRefGoogle Scholar
  21. Pérombelon, M. C. M., & Kelman, A. (1980). Ecology of the soft rot erwinias. Annual Review of Phytopathology, 18, 361–387.CrossRefGoogle Scholar
  22. Pérombelon, M. C. M. (1992). Potato blackleg: Epidemiology, host–pathogen interaction and control. Netherlands Journal of Plant Pathology, 98(suppl. 2), 135–146.CrossRefGoogle Scholar
  23. Powelson, M. L., & Apple, J. D. (1984). Soil and seed tubers as sources of inoculum of Erwinia carotovora pv. carotovora for stem soft of potatoes. Phytopathology, 74, 429–432.CrossRefGoogle Scholar
  24. Samson, R., Legendre, J. B., Christen, R., Saux, M. F. L., Achouak, W., & Gardan, L. (2005). Transfer of Pectobacterium chrysanthemi (Burkholder et al. 1953; Brenner et al. 1973) and Brenneria paradisiaca to the genus Dickeya gen. nov. as Dickeya chrysanthemi comb. nov. and Dickeya paradisiaca comb. nov. and delineation of four novel species, Dickeya dadantii sp. nov., Dickeya dianthicola sp. nov., Dickeya dieffenbachiae sp. nov. and Dickeya zeae sp. nov. International Journal of Systematic and Evolutionary Microbiology, 55, 1415–1427.PubMedCrossRefGoogle Scholar
  25. Smith, C., & Bartz, J. A. (1990). Variation in pathogenicity and aggressiveness of strains of Erwinia carotovora subsp. carotovora isolated from different hosts. Plant Disease, 74, 505–509.CrossRefGoogle Scholar
  26. Togashi, J., Ueda, K., & Namai, T. (2001). Overwintering of Erwinia carotovora subsp. carotovora in diseased tissues in soil and its role as inoculum for soft rot of Chinese cabbage (Brassica campestris, Pekinensis group). Journal of General Plant Pathology, 67, 45–50.CrossRefGoogle Scholar
  27. Toth, I. K., Sullivan, L., Brierley, J. L., Avrova, A. O., Hyman, L. J., Holeva, M., et al. (2003). Relationship between potato seed tuber contamination by Erwinia carotovora spp. atroseptica, blackleg disease development and progeny tuber contamination. Plant Pathology, 52, 119–126.CrossRefGoogle Scholar
  28. Van Beckhoven, J. R. C. M., De Haan, E. G., Van Hoof, R., De Raaij-Wieringa, G., Bonants. P., Van de Wolf, J. M. et al. (2001). Survey naar serogroepen van Erwinia carotovora subsp. atroseptica en Erwinia chrysanthemi in aardappel in Nederland. Plant Research International. Internal report. 21 pages.Google Scholar
  29. Van der Wolf, J. M., Van der Zouwen, P. S., Van Beckhoven, J. R. C. M., Speksnijder, A. G. C. L., De Haan, E. G., Boons, C. C. A. et al. (2003). Erwinia carotovora subsp. carotovora als primaire veroorzaker van zwartbenigheid in de aardappel. Plant Research International. Internal report. 38 pagesGoogle Scholar
  30. Yap, M. N., Barak, J. D., & Charkowski, A. M. (2004). Genomic diversity of Erwinia carotovora subsp. carotovora and its correlation with virulence. Applied and Environmental Microbiology, 70, 3013–3023.PubMedCrossRefGoogle Scholar

Copyright information

© KNPV 2008

Authors and Affiliations

  • Eisse G. de Haan
    • 1
  • Toos C. E. M. Dekker-Nooren
    • 1
  • Gé W. van den Bovenkamp
    • 1
  • Arjen G. C. L. Speksnijder
    • 2
  • Patricia S. van der Zouwen
    • 2
  • Jan M. van der Wolf
    • 2
  1. 1.Dutch General Inspection Service for Agricultural seed and seed potatoes (NAK)EmmeloordThe Netherlands
  2. 2.Plant Research InternationalWageningenThe Netherlands

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