European Journal of Plant Pathology

, Volume 122, Issue 1, pp 185–195 | Cite as

Beta-aminobutyric acid-induced resistance in grapevine against downy mildew: involvement of pterostilbene

  • Ana R. Slaughter
  • Mollah Md. Hamiduzzaman
  • Katia Gindro
  • Jean-Marc Neuhaus
  • Brigitte Mauch-Mani
Article

Abstract

BABA, a non-protein amino acid, was used to induce resistance in grapevine against downy mildew. BABA-induced resistance was observed in the susceptible cv. Chasselas as well as in the resistant cv. Solaris. Following BABA treatment, sporulation of Plasmopara viticola was strongly reduced and the accumulation of stilbenes increased with time following infection. Induction of trans-piceide, trans-resveratrol and, more importantly, of trans-ɛ- and trans-δ-viniferin and trans-pterostilbene was observed in BABA-primed Chasselas. On the other hand, induction of trans-resveratrol, trans δ-viniferin and trans-pterostilbene was observed in BABA-primed Solaris. The accumulation of stilbenes in BABA-primed Solaris was much higher than that found in BABA-primed Chasselas. Furthermore, BABA-treatment of Solaris led to a rapid increase in transcript levels of three genes involved in the phenylpropanoid pathway: phenylalanine ammonia lyase, cinnamate-4-hydroxylase and stilbene synthase. BABA-primed Chasselas showed increased transcript levels for cinnamate-4-hydroxylase and stilbene synthase. Here we show that pre-treatment of a susceptible grapevine cultivar with BABA prior to infection with P. viticola primed the accumulation of specific phytoalexins that are undetectable in non-BABA-primed plants. As a result, the susceptible cultivar became more resistant to downy mildew.

Keywords

Phytoalexin Plasmopara viticola Priming Stilbenes Vitis vinifera 

Abbreviations

BABA

beta aminobutyric acid

BABA-IR

BABA-induced resistance

Notes

Acknowledgements

This project was funded by the National Centre of Competence in Research (NCCR) Plant Survival, a research programme of the Swiss National Science Foundation. We thank Mr. J. Taillens (Agroscope-RAC, Changins, Nyon) for the grapevine cutting production and Dr. S. Godard (Agroscope-RAC, Changins, Nyon) for the valuable help with the measurements of phytoalexins.

References

  1. Bate, N. J., Orr, J., Ni, W., Meroni, A., Nadler-Hassar, T., Doerner, P. W., et al. (1994). Quantitative relationship between phenylalanine ammonia-lyase levels and phenylpropanoid accumulation in transgenic tobacco identifies a rate determining step in natural product synthesis. Proceedings of the National Academy of Sciences of the United States of America, 91, 7608–7612.PubMedCrossRefGoogle Scholar
  2. Blount, J. W., Korth, K. L., Masoud, S. A., Rasmussen, S., Lamb, C., & Dixon, R. A. (2000). Altering expression of cinnamic acid 4-hydroxylase in transgenic plants provides evidence for a feedback loop at the entry point into the phenylpropanoid pathway. Plant Physiology, 122, 107–116.PubMedCrossRefGoogle Scholar
  3. Cohen, Y. (2002). Beta-aminobutyric acid-induced resistance against plant pathogens. Plant Disease, 86, 448–457.CrossRefGoogle Scholar
  4. Cohen, Y., & Gisi, U. (1994). Systemic translocation of 14C-DL-3-aminobutyric acid in tomato plants in relation to induced resistance against Phytophthora infestans. Physiological and Molecular Plant Pathology, 45, 441–456.CrossRefGoogle Scholar
  5. Conrath, U., Pieterse, C. M. J., & Mauch-Mani, B. (2002). Priming in plant–pathogen interactions. Trends in Plant Science, 7, 210–216.PubMedCrossRefGoogle Scholar
  6. Dai, G. H., Andary, C., Mondolot-Cosson, L., & Boubals, D. (1995). Histochemical studies on the interaction between three species of grapevine, Vitis vinifera, V. rupestris and V. rotundifolia and the downy fungus, Plasmopara viticola. Physiological and Molecular Plant Pathology, 46, 177–188.CrossRefGoogle Scholar
  7. Dercks, W., & Creasy, L. L. (1989). The significance of stilbene phytoalexins in the Plasmopara viticola–grapevine interaction. Physiological and Molecular Plant Pathology, 34, 189–202.CrossRefGoogle Scholar
  8. Derckel, J. P., Baillieul, F., Manteau, S., Audran, J. C., Haye, B., Lambert, L., & Legendre, L. (1999). Differential induction of grapevine defences by two strains of Botrytis cinerea. Phytopathology, 89, 197–203.CrossRefPubMedGoogle Scholar
  9. Gindro, K., Pezet, R., & Viret, O. (2003). Histological study of the response of two Vitis vinifera cultivars (resistant and susceptible) to Plasmopara viticola infections. Plant Physiology and Biochemistry, 41, 846–853.CrossRefGoogle Scholar
  10. Hamiduzzaman, M. M., Jakab, G., Barnavon, L., Neuhaus, J.-M., & Mauch-Mani, B. (2005). b-amino butyric acid-induced resistance against downy mildew in grapevine acts through the potentiation of callose formation and JA signalling. Molecular Plant–Microbe Interactions, 18, 819–829.PubMedCrossRefGoogle Scholar
  11. Howles, P. A., Paiva, N. L., Sewalt, V. J. H., Elkind, N. L., Bate, Y., Lamb, C. J., et al. (1996). Over-expression of l-phenylalanine ammonia-lyase in transgenic tobacco plants reveals control points for flux into phenylpropanoid biosynthesis. Plant Physiology, 112, 1617–1624.PubMedGoogle Scholar
  12. Iandolino, A. B., da Silva, F. G., Lim, H., Choi, H., Williams, L. E., & Cook, D. R. (2004). High-quality RNA, cDNA, and derived EST libraries from grapevine (Vitis vinifera L.). Plant Molecular Biology Reporter, 22, 269–278.CrossRefGoogle Scholar
  13. Jakab, G., Cottier, V., Toquin, V., Rigoli, G., Zimmerli, L., Metraux, J. P., et al. (2001). Beta-aminobutyric acid-induced resistance in plants. European Journal of plant pathology, 107, 29–37.CrossRefGoogle Scholar
  14. Jeandet, P., Douillet-Breuil, A. C., Bressis, R., Debord, S., Spaghi, M., & Adrian, M. (2002). Phytoalexins from the Vitaceae: Biosynthesis, phytoalexin gene expression in transgenic plants, antifungal activity, and metabolism. Journal of Agricultural and food chemistry, 50, 2731–2741.PubMedCrossRefGoogle Scholar
  15. Keogh, R. C., Deverall, B. J., & McLeod, S. (1980). Comparison of histological and physiological responses to Phakopsora pachyrhizi in resistant and susceptible soybean. Transactions of the British Mycological Society, 74, 329–333.CrossRefGoogle Scholar
  16. Kuc, J. (1995). Phytoalexin, stress metabolism, and disease resistance in plants. Annual Review of Phytopathology, 33, 275–297.CrossRefPubMedGoogle Scholar
  17. Langcake, P. (1981). Disease resistance of Vitis spp. and the production of the stress metabolites resveratrol, e-viniferin, a-viniferin and pterostilbene. Physiological Plant Pathology, 18, 213–226.Google Scholar
  18. Liswidowati, F., Melchior, F., Hohmann, F., Schwer, B., & Kindl, H. (1991). Induction of stilbene synthase by Botrytis cinerea in cultured grapevine cells. Planta, 183, 307–314.CrossRefGoogle Scholar
  19. Matasci, C. L., Gobbin, D., Schärer, H.-J., Tamm, L., & Gessler, C. (2008). Selection for fungicide resistance throughout a growing season in populations of Plasmopara viticola. European Journal of Plant Pathology, 120, 79–83.CrossRefGoogle Scholar
  20. Pezet, R., Gindro, K., Viret, O., & Richter, H. (2004b). Effects of resveratrol, viniferins and pterostilbene on Plasmopara viticola zoospore mobility and disease development. Vitis, 43, 145–148.Google Scholar
  21. Pezet, R., Gindro, K., Viret, O., & Spring, J.-L. (2004a). Glycosylation and oxidative dimerization of resveratrol are respectively associated to sensitivity and resistance of grapevine cultivars to downy mildew. Physiological and Molecular Plant Pathology, 65, 297–303.CrossRefGoogle Scholar
  22. Pezet, R., Perret, C., Jean-Denis, J. B., Tabacchi, R., Gindro, K., & Viret, O. (2003). d-viniferin, a resveratrol dehydrodimer: One of the major stilbenes synthesized by grapevine leaves. Journal of Agricultural and Food Chemistry, 51, 5488–5492.PubMedCrossRefGoogle Scholar
  23. Prime-A-Plant Group; Conrath, U., Beckers, G. J., Flors, V., Garcia-Agustin, P., Jakab, G., et al. (2006). Priming: Getting ready for battle. Molecular Plant–Microbe Interactions, 19, 1062–1071.PubMedCrossRefGoogle Scholar
  24. Sewalt, V. J. H., Ni, W., Blount, J. W., Jung, H. G., Howles, P. A., Masoud, S. A., et al. (1997). Reduced lignin content and altered lignin composition in transgenic tobacco down-regulated in expression of phenylalanine ammonia-lyase or cinnamate 4-hydroxylase. Plant Physiology, 115, 41–50.PubMedGoogle Scholar
  25. Sgarbi, E., Fornassiero, R. B., Lins, A. P., & Bonatti, P. M. (2003). Phenol metabolism is differentially affected by ozone in two cell lines from grape (Vitis vinifera L.) leaf. Plant Science, 165, 951–957.CrossRefGoogle Scholar
  26. Solecka, D., & Kacperska, A. (2003). Phenylpropanoid deficiency affects the course of plant acclimation to cold. Physiologia Plantarum, 119, 253–262.CrossRefGoogle Scholar
  27. Sticher, L., Mauch-Mani, B., & Metraux, J. P. (1997). Systemic acquired resistance. Annual Review of Phytopathology, 35, 235–270.PubMedCrossRefGoogle Scholar
  28. Steiner, U., & Schönbeck, F. (1997). Induced resistance. In H. Hartleb, R. Heitefuss, & H. H. Hopp (Eds.) Resistance of crop plants against fungi (pp. 272–297). Lubeck, Ulm: Gustav Fischer, Jena, Stuttgart.Google Scholar
  29. Vandesompele, J., De Preter, K., Pattyn P., Poppe, B., Van Roy, N., De Paepe, A., et al. (2002). Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biology, 3, research0034.1–research0034.11, Retrieved from http://genomebiology.com/2002/3/7/research/0034.1.
  30. Wiese, W., Vornam, B., Krause, E., & Kindl, H. (1994). Structural organization and differential expression of three stilbene synthase genes located on a 13 kb grapevine DNA fragment. Plant Molecular Biology, 26, 667–677.PubMedCrossRefGoogle Scholar

Copyright information

© KNPV 2008

Authors and Affiliations

  • Ana R. Slaughter
    • 1
  • Mollah Md. Hamiduzzaman
    • 1
    • 2
  • Katia Gindro
    • 3
  • Jean-Marc Neuhaus
    • 1
  • Brigitte Mauch-Mani
    • 1
  1. 1.Laboratory of Molecular and Cellular BiologyUniversity of NeuchâtelNeuchâtelSwitzerland
  2. 2.Department of Plant Physiology and Botany DepartmentStockholm UniversityStockholmSweden
  3. 3.Swiss Federal Research Station for Plant Production of Changins-Wädenswil ACWNyonSwitzerland

Personalised recommendations