Beta-aminobutyric acid-induced resistance in grapevine against downy mildew: involvement of pterostilbene
- 373 Downloads
BABA, a non-protein amino acid, was used to induce resistance in grapevine against downy mildew. BABA-induced resistance was observed in the susceptible cv. Chasselas as well as in the resistant cv. Solaris. Following BABA treatment, sporulation of Plasmopara viticola was strongly reduced and the accumulation of stilbenes increased with time following infection. Induction of trans-piceide, trans-resveratrol and, more importantly, of trans-ɛ- and trans-δ-viniferin and trans-pterostilbene was observed in BABA-primed Chasselas. On the other hand, induction of trans-resveratrol, trans δ-viniferin and trans-pterostilbene was observed in BABA-primed Solaris. The accumulation of stilbenes in BABA-primed Solaris was much higher than that found in BABA-primed Chasselas. Furthermore, BABA-treatment of Solaris led to a rapid increase in transcript levels of three genes involved in the phenylpropanoid pathway: phenylalanine ammonia lyase, cinnamate-4-hydroxylase and stilbene synthase. BABA-primed Chasselas showed increased transcript levels for cinnamate-4-hydroxylase and stilbene synthase. Here we show that pre-treatment of a susceptible grapevine cultivar with BABA prior to infection with P. viticola primed the accumulation of specific phytoalexins that are undetectable in non-BABA-primed plants. As a result, the susceptible cultivar became more resistant to downy mildew.
KeywordsPhytoalexin Plasmopara viticola Priming Stilbenes Vitis vinifera
beta aminobutyric acid
This project was funded by the National Centre of Competence in Research (NCCR) Plant Survival, a research programme of the Swiss National Science Foundation. We thank Mr. J. Taillens (Agroscope-RAC, Changins, Nyon) for the grapevine cutting production and Dr. S. Godard (Agroscope-RAC, Changins, Nyon) for the valuable help with the measurements of phytoalexins.
- Bate, N. J., Orr, J., Ni, W., Meroni, A., Nadler-Hassar, T., Doerner, P. W., et al. (1994). Quantitative relationship between phenylalanine ammonia-lyase levels and phenylpropanoid accumulation in transgenic tobacco identifies a rate determining step in natural product synthesis. Proceedings of the National Academy of Sciences of the United States of America, 91, 7608–7612.PubMedCrossRefGoogle Scholar
- Blount, J. W., Korth, K. L., Masoud, S. A., Rasmussen, S., Lamb, C., & Dixon, R. A. (2000). Altering expression of cinnamic acid 4-hydroxylase in transgenic plants provides evidence for a feedback loop at the entry point into the phenylpropanoid pathway. Plant Physiology, 122, 107–116.PubMedCrossRefGoogle Scholar
- Dai, G. H., Andary, C., Mondolot-Cosson, L., & Boubals, D. (1995). Histochemical studies on the interaction between three species of grapevine, Vitis vinifera, V. rupestris and V. rotundifolia and the downy fungus, Plasmopara viticola. Physiological and Molecular Plant Pathology, 46, 177–188.CrossRefGoogle Scholar
- Hamiduzzaman, M. M., Jakab, G., Barnavon, L., Neuhaus, J.-M., & Mauch-Mani, B. (2005). b-amino butyric acid-induced resistance against downy mildew in grapevine acts through the potentiation of callose formation and JA signalling. Molecular Plant–Microbe Interactions, 18, 819–829.PubMedCrossRefGoogle Scholar
- Jeandet, P., Douillet-Breuil, A. C., Bressis, R., Debord, S., Spaghi, M., & Adrian, M. (2002). Phytoalexins from the Vitaceae: Biosynthesis, phytoalexin gene expression in transgenic plants, antifungal activity, and metabolism. Journal of Agricultural and food chemistry, 50, 2731–2741.PubMedCrossRefGoogle Scholar
- Langcake, P. (1981). Disease resistance of Vitis spp. and the production of the stress metabolites resveratrol, e-viniferin, a-viniferin and pterostilbene. Physiological Plant Pathology, 18, 213–226.Google Scholar
- Pezet, R., Gindro, K., Viret, O., & Richter, H. (2004b). Effects of resveratrol, viniferins and pterostilbene on Plasmopara viticola zoospore mobility and disease development. Vitis, 43, 145–148.Google Scholar
- Sewalt, V. J. H., Ni, W., Blount, J. W., Jung, H. G., Howles, P. A., Masoud, S. A., et al. (1997). Reduced lignin content and altered lignin composition in transgenic tobacco down-regulated in expression of phenylalanine ammonia-lyase or cinnamate 4-hydroxylase. Plant Physiology, 115, 41–50.PubMedGoogle Scholar
- Steiner, U., & Schönbeck, F. (1997). Induced resistance. In H. Hartleb, R. Heitefuss, & H. H. Hopp (Eds.) Resistance of crop plants against fungi (pp. 272–297). Lubeck, Ulm: Gustav Fischer, Jena, Stuttgart.Google Scholar
- Vandesompele, J., De Preter, K., Pattyn P., Poppe, B., Van Roy, N., De Paepe, A., et al. (2002). Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biology, 3, research0034.1–research0034.11, Retrieved from http://genomebiology.com/2002/3/7/research/0034.1.