European Journal of Plant Pathology

, Volume 118, Issue 4, pp 393–400 | Cite as

In vitro screening for sunflower (Helianthus annuusL.) resistant calli to Diaporthe helianthi fungal culture filtrate

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Abstract

Diaporthe helianthi the causal agent of sunflower (Helianthus annuus) stem canker, causes significant reductions in yield and oil content in most sunflower-growing areas. With the aim of enhancing host resistance, we selected in vitro sunflower calli against culture filtrates of two pathogen isolates (7/96 and 101/96). This technique may be an effective and rapid tool to discriminate the most virulent D. helianthi isolate and to screen for host resistance in the early stage of a breeding programme. Further investigation on the mechanisms involved in defence pathways showed no induction of salicylic acid and pathogenesis-related proteins in calli, indicating that the host resistance is not associated with Systemic Acquired Resistance but probably other biochemical mechanisms.

Keywords

Benzothiadiazole Pathogenesis-related proteins Diaporthe helianthi Salicylic acid Calli selection 

Abbreviations

CI

Callus induction medium

CM

Callus maintenance medium

2,4 D

2,4 Dichlorophenoxyacetic acid

MA

Malt agar

MS

Murashige & Skoog’s medium

NAA

α-Naphthalenacetic acid

PR proteins

Pathogenesis-related proteins

SA

Salicylic acid

SAR

Systemic acquired resistance

V.I.

Variation index

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Copyright information

© KNPV 2007

Authors and Affiliations

  1. 1.Department of Agricultural and Environmental ScienceUniversity of PerugiaPerugiaItaly

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