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European Journal of Plant Pathology

, Volume 116, Issue 1, pp 33–43 | Cite as

Development of an effective screening method for partial resistance to Alternaria brassicicola (dark leaf spot) in Brassica rapa

  • M. A. U. Doullah
  • M. B. Meah
  • K. OkazakiEmail author
Article

Abstract

In order to develop a method to measure resistance to Alternaria brassicicola (cause of dark leaf spot disease) in Brassica rapa, the effects of inoculum concentration, leaf stage, leaf age and incubation temperature of inoculation on infection were studied under controlled conditions using several B. rapa genotypes. Three inoculation methods (cotyledon, detached leaf and seedling inoculation) were evaluated for this purpose. The detached leaf inoculation test was the most suitable for screening B. rapa genotypes because clear symptoms were observed on the leaves in less than 24 h, and there was a significant positive correlation between the results from the detached leaf inoculation test and the seedling inoculation test, an established method considered to yield reliable results. In addition, it was very easy to screen plants for resistance on a large scale and to maintain standard physical conditions using detached leaves. For successful infection, inoculum concentration should be adjusted to 5 × 104 conidia  ml−1, and incubation temperature should be between 20 °C and 25 °C. The 3rd/4th true leaves from 30 day-old plants were optimal for inoculation. In a screening test using 52 cultivars of B. rapa, the detached leaf test effectively discriminated between various levels of partial resistance among cultivars. As a result, we identified two cultivars, viz Saori and Edononatsu, as highly resistant and five cultivars, viz Tokinashi Taisai, Yajima Kabu, Purara, Norin-F1-Bekana and Tateiwa Kabu, as having borderline resistance.

Keywords

inoculum concentration incubation temperature inoculation technique leaf position leaf age rapeseed 

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Notes

Acknowledgements

The authors are grateful to the seed companies of Japan, Gene Bank of the National Institute of Agrobiological Resources, Japan, and Bangladesh Agricultural Research Institute, Bangladesh, for providing seeds of Brassica rapa. The authors sincerely thank Dr. Y. Sato of Toyama Prefectural University, Toyama, Japan and, Dr. T. Shirakawa of the National Institute of Vegetable and Tea, Tsukuba, Japan, and Gazi M. Mohsin from East West seed (Bangladesh) Ltd., Bangladesh, for their valuable suggestions. The first author is supported by a scholarship from the Ministry of Education, Culture, Sports, Science and Technology, Government of Japan (Monbukagakusho-MEXT).

References

  1. Bassey EO, Gabrielson RL (1983) The effects of humidity, seed infection level, temperature and nutrient stress on cabbage seedling disease caused by Alternaria brassicicola. Seed Science and Technology 11: 403–410Google Scholar
  2. Changsri W, Weber GF (1963) Three Alternaria species pathogenic on certain cultivated crucifers. Phytopathology 53: 643–648Google Scholar
  3. Degenhardt KJ, Petrie GA, Morrall RAA (1982) Effects of temperature on spore germination and infection of rapeseeds by Alternaria brassicae, A. brassicicola, and A. raphani. Canadian Journal of Plant Pathology 4: 115–118CrossRefGoogle Scholar
  4. Dueck J and Degenhardt K (1975) Effect of leaf age and inoculum concentration on reaction of oilseed Brassica spp. to Alternaria brassicae. (Abstract) Proceedings of the American Phytopathological Society 2: 59Google Scholar
  5. Hansen LN, Earle ED (1997) Somatic hybrids between Brassica oleracea L. and Sinapis alba L. with resistance to Alternaria brassicae (Berk.) Sacc. Theoretical and Applied Genetics 94:1078–1085CrossRefGoogle Scholar
  6. Hong CX, Fitt BDL (1995) Effect of inoculum concentration, leaf age and wetness period on the development of dark leaf and pod spot (Alternaria brassicae) on oilseed rape (Brassica napus). Annals of Applied Biology 127: 183–295CrossRefGoogle Scholar
  7. Hong CX, Fitt BDL, Welham SJ (1996) Effect of wetness period and temperature on development of dark pod spot (Alternaria brassicae) on oilseed rape (Brassica napus). Plant Pathology 45: 1077–1089CrossRefGoogle Scholar
  8. Humpherson-Jones FM (1985) The incidence of Alternaria spp. and Leptosphaeria maculans in commercial brassica seed in United Kingdom. Plant Pathology 34: 385–390CrossRefGoogle Scholar
  9. ISTA (1996) International Rules for Seed Testing. Seed Science and Technology 4: 3–49Google Scholar
  10. Ishida M, Takahata Y, Kaizuma N (2003) Simple and rapid method for the selection of individual rapeseed plants low in glucosinolates. Breeding Science 53:291–296CrossRefGoogle Scholar
  11. Jasalavich CA, Seguin-Swartz G, Vogelgsang S, Petrie GA (1993) Host range of Alternaria species pathogenic to crucifers. Canadian Journal of Plant Pathology 15: 314–315Google Scholar
  12. Kim SJ, Ishida M, Matsuo T, Watanabe M, Watanabe Y (2001) Separation and identification of glucosinolates of vegetable turnip rapa by LC/APCI-MS and comparison of their contents in ten cultivars of vegetable turnip rape (Brassica rapa L.). Soil Science and Plant Nutrition 1: 167–177Google Scholar
  13. King SR (1994) Screening, selection, and genetics of resistance to Alternaria diseases in Brassica oleracea. Ph.D. thesis, Cornell University, Ithaca, New York, 128 ppGoogle Scholar
  14. Kubota M, Abiko K, Nishi K (2003) Effect of cultivation conditions of cabbage plugs on sooty spot disease. Bulletin of the National Institute of Vegetable and Tea Science, Japan 2: 1–8Google Scholar
  15. Meah MB, Howlider MAR, Alam MK (1988) Effect of fungicide spray at different times and frequencies on Alternaria blight of mustard. Thai Journal of Agricultural Science 21: 101–107Google Scholar
  16. Meah MB, Hau B, Siddique MK (2002) Relationships between disease parameters of Alternaria blight (Alternaria brassicae) and yield of mustard. Journal of Plant Diseases and Protection 3: 243–251Google Scholar
  17. Milford GFJ, Fieldsend JK, Porter AJR, Rawlinson CJ, Evans EJ, Bilsborrow P (1989) Changes in glucosinolate concentrations during the vegetative growth of single and double low cultivars of winter oilseed rape. Aspects of Applied Biology 23: 83–90Google Scholar
  18. Mridha MAU, Wheeler BEJ (1993) In vitro effects of temperature and wet periods on infection of oilseed rape by Alternaria brassicae. Plant Pathology 42: 671–675CrossRefGoogle Scholar
  19. Rotem J (1998) The Genus Alternaria: Biology, Epidemiology, and Pathology. APS Press, The American Phytopathological Society, Minnesota, USA, 326 ppGoogle Scholar
  20. Schimmer FC (1953) Alternaria brassicicola on summer cauliflower seed. Plant Pathology 2: 16–17CrossRefGoogle Scholar
  21. Sharma G, Kumar DV, Haque A, Bhat SR, Prakash S, Chopra VL (2002) Brassica coenospecies: a rich reservoir for genetic resistance to leaf spot caused by Alternaria brassicae. Euphytica 125: 411–417CrossRefGoogle Scholar
  22. Surujdeo-Maharaj S, Umaharan P, Butler DR, Sreenivasan TN (2003) An optimized screening method for identifying levels of resistance to Crinipellis perniciosa in cocoa (Theobroma cacao). Plant Pathology 52: 464–475CrossRefGoogle Scholar
  23. Tewari JP, Mithen RF (1999) Disease. In: Gomez-Campo C (eds) Biology of Brassica coenospecies. Elsevier, Amsterdam, The Netherlands, pp 375–411CrossRefGoogle Scholar
  24. Vishwanath and Kolte SJ (1999) Methods of inoculation for resistance to Alternaria blight of rapeseed and mustard. Journal of Mycology and Plant Pathology 29: 96–99Google Scholar
  25. Westman AL, Kresovich S, Dickson MH (1999) Regional variation in Brassica nigra and other weedy crucifers for disease reaction to Alternaria brassicicola and Xanthomonas campestris pv.campestris. Euphytica 106: 253–259CrossRefGoogle Scholar

Copyright information

© Springer 2006

Authors and Affiliations

  1. 1.Faculty of AgricultureNiigata UniversityNiigataJapan
  2. 2.Department of Plant PathologyBangladesh Agricultural UniversityMymensinghBangladesh

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