European Journal of Plant Pathology

, Volume 115, Issue 3, pp 363–366

The Use of Bioluminescence for Monitoring in planta Growth Dynamics of a Pseudomonas syringae Plant Pathogen

  • Christopher D. Paynter
  • Vyvyan C. Salisbury
  • Dawn L. Arnold
  • Robert W. Jackson
Short Communication

Abstract

The use of bioluminescence was evaluated as a tool to study Pseudomonas syringae population dynamics in susceptible and resistant plant environments. Plasmid pGLITE, containing the luxCDABE genes from Photorhabdus luminescens, was introduced into Pseudomonassyringae pv. phaseolicola race 7 strain 1449B, a Gram-negative pathogen of bean (Phaseolus vulgaris). Bacteria recovered from plant tissue over a five-day period were enumerated by counting numbers of colony forming units and by measurement of bioluminescence. Direct measurement of bioluminescence from leaf disc homogenates consistently reflected bacterial growth as determined by viable counting, but also detected subtle effects of the plant resistance response on bacterial viability. This bioluminescence procedure enables real time measurement of bacterial metabolism and population dynamics in planta, obviates the need to carry out labour intensive and time consuming traditional enumeration techniques and provides a sensitive assay for studying plant effects on bacterial cells.

Keywords

Avirulence bacteria bean lux pathogenicity 

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Copyright information

© Springer 2006

Authors and Affiliations

  • Christopher D. Paynter
    • 1
    • 2
  • Vyvyan C. Salisbury
    • 1
  • Dawn L. Arnold
    • 1
  • Robert W. Jackson
    • 1
    • 3
  1. 1.Faculty of Applied SciencesUniversity of the West of EnglandFrenchayUK
  2. 2.Department of Environmental ScienceLancaster UniversityLancasterUK
  3. 3.Department of Biology and BiochemistryUniversity of BathBathUK

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