European Journal of Plant Pathology

, Volume 114, Issue 3, pp 301–308 | Cite as

Assessment of Partial Resistance to Powdery Mildew (Podosphaera pannosa) in a Tetraploid Rose Population Using a Spore-suspension Inoculation Method

  • Zifu Yan
  • Oene Dolstra
  • Theo W. Prins
  • Piet Stam
  • Peter B. Visser
Article
Accepted:

Abstract

Powdery mildew (Podosphaera pannosa) is the most important fungal disease in greenhouse roses and is in practice controlled by fungicides. The creation of novel cultivars with durable resistance to powdery mildew is highly desirable. To understand the inheritance of mildew resistance, a tetraploid rose population with a size of 181 seedlings was obtained by crossing two tetraploid genotypes each having partial resistance. The population and its parents were tested under greenhouse conditions with two well-defined monospore isolates (2 and F1) using artificial inoculation with spore suspensions. Disease score at 11 days post-inoculation, latent period and rate of symptom development were used to describe seedling resistance. The tests for both isolates exhibited a wide and significant variation among genotypes for resistance. The distribution of the genotypic means of the disease scores was continuous and showed a considerable transgression. Statistical analysis, scatter plot of disease scores for the isolates, and correlation analyses indicated that the two isolates differed in pathogenicity. The outcome of the tests showed that the inoculation assay with spore suspensions was a reliable and effective way to screen large numbers of genotypes under greenhouse conditions for genetic and breeding studies. This is the first report on spore-suspension inoculation to be used successfully in rose.

Keywords

durable resistance greenhouse test latent period monospore isolate Rosa 
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References

  1. Bai, Y, Huang, CC, Hulst, R, Meijer-Dekens, F, Bonnema, G, Lindhout, P 2003QTLs for tomato powdery mildew resistance (Oidium lycopersici) in Lycopersicon parviflorum G1.1601 co-localize with two qualitative powdery mildew resistance genesMolecular Plant–Microbe Interactions16169176PubMedGoogle Scholar
  2. Bai Y (2004) The genetics and mechanisms of resistance to tomato powdery mildew (Oidium lycopersici) in Lycopersicon species. PhD thesis, Wageningen University, Wageningen, The NetherlandsGoogle Scholar
  3. Bender, CL, Coyier, DL 1984Isolation and identification of races of Sphaerotheca pannosa var. rosae Phytopathology73100103Google Scholar
  4. Conti, GG, Bassi, M, Maffi, D, Bonecchi, R 1985Host–parasite relationship in a susceptible and a resistant rose cultivar inoculated with Sphaerotheca pannosa. I. Fungal growth, mechanical barriers and hypersensitive responsePhytopathology1137180Google Scholar
  5. Vries, DP, Dubois, LAM 2001Developments in breeding for horizontal and vertical fungus resistance in rosesActa Horticulturae552103112Google Scholar
  6. Falak, I, Falk, DE, Tinker, NA, Mather, DE 1999Resistance to powdery mildew in a doubled haploid barley population and its association with marker lociEuphytica107185192CrossRefGoogle Scholar
  7. Ferrero, F, Cadour-Marvaldi, P, Jacob, Y, Coudret, A, Sallanon, H, Urban, L 2001Evaluation of the resistance to powdery mildew, Sphaerotheca pannosa var. rosae, of rose-tree species and hybridsActa Horticulturae547379381Google Scholar
  8. Francisco, JB, Reifschneider, FJB, Boiteux, LS 1988A vacuum-operating setting tower for inoculation of powdery mildew fungiThe American Phytopathology Society7814631465Google Scholar
  9. Frinking, HD, Verweij, R 1989Colonization of rose by Sphaerotheca pannosa (Wallr.) Lev. var. rosae WorNetherlands Journal of Plant Pathology956372CrossRefGoogle Scholar
  10. Jorgensen, JH 1994Genetics of powdery mildew resistance in barleyCritical Reviews in Plant Sciences1397119Google Scholar
  11. Lefebvre, V, Daubeze, AM, Voort, RJ, Peleman, J, Bardin, M, Palloix, A 2003QTLs for resistance to powdery mildew in pepper under natural and artificial infectionsTheoretical and Applied Genetics107661666PubMedCrossRefGoogle Scholar
  12. Leus, L, Huylenbroeck, , Bockstaele, E 2002Powdery mildew on roses: Pathotype screeningActa Horticulturae5729195Google Scholar
  13. Leus, L, Huylenbroeck, , Bockstaele, E, Hofte, M 2003Bioassays for resistance screening in commercial rose breedingActa Horticulturae6123945Google Scholar
  14. Linde, M, Debener, T 2003Isolation and identification of eight races of powdery mildew of roses (Podosphaera pannosa) (Wallr.:Fr.) de Bary and the genetic analysis of the resistance gene Rpp1 Theoretical and Applied Genetics107256262PubMedCrossRefGoogle Scholar
  15. Linde, M, Shishkoff, N 2003Powdery mildewRoberts, ADebener, TGudin, S eds. Encyclopedia of Rose SciencesElsevier ScienceOxford158165Google Scholar
  16. Linde, M, Mattiesch, L, Debener, T 2004 Rpp1, a dominant gene providing race-specific resistance to rose powdery mildew (Podosphaera pannosa): Molecular mapping, SCAR development and confirmation of disease resistance dataTheoretical and Applied Genetics10912611266PubMedCrossRefGoogle Scholar
  17. Lindhout, P 2002The perspectives of polygenic resistance in breeding for durable disease resistanceEuphytica124217226CrossRefGoogle Scholar
  18. Mence, M, Hildebrandt, AC 1966Resistance to powdery mildew in roseAnnals of Applied Biology58309320CrossRefGoogle Scholar
  19. Mingeot, D, Chantret, N, Baret, PV, Dekeyser, A, Bouphatem, N, Sourdille, P, Doussinault, G, Jacquemin, JM 2002Mapping QTL involved in adult plant resistance to powdery mildew in the winter wheat line RE714 in two susceptible genetic backgroundsPlant Breeding12133140CrossRefGoogle Scholar
  20. Payne, R, Murray, D, Harding, S, Baird, D, Soutar, D, Lane, P 2002GenStat® for WindowsTM (6th Edition) IntroductionVSN InternationalOxfordGoogle Scholar
  21. Schiff, CL, Wilson, IW, Somerville, SC 2001Polygenic powdery mildew disease resistance in Arabidopsis thaliana: Quantitative trait analysis of the accession Warschau-1Plant Pathology50690701CrossRefGoogle Scholar
  22. Schlosser, E 1990Horizontal resistance of some plant species to powdery mildewMededelingen Faculteit Landbouw Rijksuniversiteit Gent55203206Google Scholar
  23. Sivapalan, A 1993Effects of water on germination of powdery mildew conidiaMycological Research977176CrossRefGoogle Scholar
  24. Temmen, KH, Gruppe, W, Schlosser, E 1980Investigation on the resistance of plants to powdery mildew. V. Basis for horizontal resistance of Rosa against Sphaerotheca pannosa Mededelingen Faculteit Landbouw Rijksuniversiteit Gent45153158Google Scholar
  25. Wheeler, BEJ 1973Research on rose powdery mildew at Imperial CollegeJournal of the Royal Horticultural Society15225230Google Scholar
  26. Willams, KJ 2003The molecular genetics of disease resistance in barleyAustralian Journal of Agricultural Research5410651079CrossRefGoogle Scholar
  27. Xu, X-M 1999Effects of temperature on the latent period of the rose powdery mildew pathogen, Sphaerotheca pannosa Plant Pathology48662667CrossRefGoogle Scholar
  28. Yarwood, CE 1939Control of powdery mildews with a water sprayPhytopathology29282284Google Scholar
  29. Zijlstra, S, Jansen, RC, Groot, SP 1995The relationship between powdery mildew (Sphaerotheca fuliginea) resistance and leaf chlorosis sensitivity in cucumber (Cucumis sativus) studied in single seed decent linesEuphytica81193198CrossRefGoogle Scholar

Copyright information

© Springer 2006

Authors and Affiliations

  • Zifu Yan
    • 1
  • Oene Dolstra
    • 1
  • Theo W. Prins
    • 1
  • Piet Stam
    • 2
  • Peter B. Visser
    • 1
    • 3
  1. 1.Plant Research International B.V.Wageningen University and Research CentreWageningenThe Netherlands
  2. 2.Laboratory of Plant BreedingWageningen University and Research CentreWageningenThe Netherlands
  3. 3.Instituto Agroforestal MediterráneoUniversidad Politécnica de ValenciaValenciaSpain

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