European Journal of Plant Pathology

, Volume 113, Issue 1, pp 107–109 | Cite as

The Database of PCR Primers for Phytopathogenic Fungi

Short communication

Abstract

We present the first on-line searchable database of primer sets useful for the detection of plant pathogenic fungi. This web resource is implemented entirely with open-source software (PHP, MySQL). Primer set details can be retrieved by organism name, primer name, nucleotide sequence comparison, target DNA, PCR technique, author name, journal and year of publication. Each record is linked directly to other reference databases to allow easy access to the correct nomenclature, taxonomical position and anamorph/teleomorph connections of the pathogen, GenBank-deposited source sequences of the primer sets and reference contents. The database is open to user contributions and can be consulted at http://www.sppadbase.com. While currently devoted to fungal organisms, in a future perspective the database may host primer sets specific to other phytopathogens, such as viruses or bacteria, including microbial herbicides and biological agents relevant in agroterrorism.

Keywords

agroterrorism bioweapon database diagnostics plant pathogenic fungi polymerase chain reaction primer sets 

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References

  1. Galperin MY (2005) The Molecular Biology Database Collection: 2005 update. Nucleic Acids Research 33: Database issue D5-D24.Google Scholar
  2. Louws, FJ, Rademaker, JLW, Bruijn, FJ 1999The three Ds of PCR-based genomic analysis of phytobacteria: Diversity, detection, and disease diagnosisAnnual Review of Phytopathology3781125CrossRefPubMedGoogle Scholar
  3. Monke J (2004) Agroterrorism: Threats and preparedness. Congressional Research Service Report RL32521 for US Congress. http://www.fas.org/irp/crs/RL32521.pdf.Google Scholar
  4. Mullis, K, Faloona, FA 1987Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reactionMethods in Enzymology1553540CrossRefGoogle Scholar
  5. Welsh, J, McClelland, M 1990Fingerprinting genomes using arbitrary primersNucleic Acids Research1872137218PubMedGoogle Scholar
  6. White, TJ, Bruns, T, Lee, S, Taylor, J 1990

    Amplification and direct sequencing of fungal ribosomal RNA Genes for phylogenetics

    Innis, NGelfand, DSninsky, JWhite, T eds. PCR – Protocols and Applications – A Laboratory ManualAcademic PressNew York315322
    Google Scholar
  7. Williams, JCK, Kubelik, AR, Livak, KJ, Rafalski, JA, Tingey, V 1990DNA polymorphisms amplified by arbitrary primers are useful as genetic markersNucleic Acids Research1865316535PubMedGoogle Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  1. 1.Plant Protection InstituteTurin Sect.–CNRTurinItaly
  2. 2.Department of Plant Protection – Center for Biotechnology Development and Biodiversity ResearchUniversity of SassariSassariItaly

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