European Journal of Plant Pathology

, Volume 111, Issue 4, pp 371–379 | Cite as

Improved immunological detection of Spongospora subterranea

  • U. Merz
  • J. A. Walsh
  • K. Bouchek-Mechiche
  • Th. Oberhänsli
  • W. Bitterlin
Article

Abstract

The genus Spongospora has two members which are important pathogens of vegetables, S. subterranea f.sp. subterranea (Sss) and S. subterranea f.sp. nasturtii (Ssn). The close taxonomic relationship of these formae speciales is based on similar cystosori morphology. The potato disease powdery scab, caused by Sss, is difficult to control. The key control measure is avoidance, aimed at planting clean seed in clean soil. For the development of routine tests for the presence of the pathogen on tubers and in soil, a monoclonal antibody (MAb) was developed using Sss cystosori as immunogen. It detected less than one Sss cystosorus and recognised Sss material from many parts of the world. No cross-reactions with other Plasmodiophoromycetes including Plasmodiophora brassicae, Polymyxa betae, Polymyxa graminis and different Streptomyces species causing common and netted scab of potatoes were observed. A novel tuber sample test method was developed using a kitchen peeling machine. This detected two tubers with one powdery scab lesion each in a sample including eighteen uninfected tubers. When soil samples spiked with cystosori were tested with the MAb, different Sss infestation levels could be discriminated. Ssn cystosori gave absorbance values in ELISA as high as Sss cystosori, whereas fresh crook roots of watercress containing Ssnzoosporangia and plasmodia or mud from an Ssninfected watercress bed gave low absorbance values or no reaction. The potential of these findings for the development of a disease control management are discussed.

Keywords

Inoculum monoclonal antibody potato quantification watercress 

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References

  1. Bell, KS, Roberts, J, Verrall, S, Cullen, DW, Williams, NA, Harrison, JG, Toth, LK, Cooke, DEL, Duncan, JM, Claxton, JR 1999Detection and quantification of Spongospora subterranea f.sp. subterranea in soils and on tubers using specific PCR primersEuropean Journal of Plant Pathology105905915Google Scholar
  2. Bouchek-Mechiche, K, Gardan, L, Normand, P, Jouan, B 2000DNA relatedness among strains of Streptomyces pathogenic to potato in France: Description of three new species, S. europaeiscabiei sp. nov. and S. stelliscabiei sp. nov. associated with common scab, and S. reticuliscabiei sp. nov. associated with netted scabInternational Journal of Systematic and Evolutionary Microbiology509199Google Scholar
  3. Braselton, JP 1995Current status of the PlasmodiophoridsCritical Reviews in Microbiology21263275PubMedGoogle Scholar
  4. Bulman, SR, Marshall, JW 1998Detection of Spongospora subterranea in potato tuber lesions using the polymerase chain reaction (PCR)Plant Pathology47759766Google Scholar
  5. Clark, MF, Adams, AN 1977Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant virusesJournal of General Virology34475483PubMedGoogle Scholar
  6. St. Groth, S, Scheidegger, D 1980Production of monoclonal antibodies: Strategy and tacticsJournal of Immunological Methods35121PubMedGoogle Scholar
  7. Down, GJ, Clarkson, JM 2002Development of a PCR-based diagnostic test for Spongospora subterranea f.sp. nasturtii, the causal agent of crook root of watercress (Rorippa nasturtium-aquaticum)Plant Pathology51275280Google Scholar
  8. Falloon, R, Genet, R, Wallace, A, Nott, H 1999IPM use for powdery scabCommercial Grower543334Google Scholar
  9. Harrison, JG, Rees, EA, Barker, H, Lowe, R 1993Detection of spore balls of Spongospora subterranea on potato tubers by enzyme-linked immunosorbent assayPlant Pathology42181186Google Scholar
  10. Lambert, DH, Loria, R 1989aStreptomyces scabies sp. nov. nomInternational Journal of Systematic Bacteriology39387392Google Scholar
  11. Lambert, DH, Loria, R 1989bStreptomyces acidiscabies sp. nov. nomInternational Journal of Systematic Bacteriology39393396Google Scholar
  12. Merz, U 1989Infectivity, inoculum density and germination of Spongospora subterranea resting spores: A solution-culture test systemEPPO Bulletin19585592Google Scholar
  13. Merz U (1993). Epidemiological aspects of powdery scab of potatoes caused by Spongospora subterranea. Proceedings of the 2nd Symposium of the International Working Group on Plant Viruses with Fungal Vectors (pp 104–106) Montreal, Canada.Google Scholar
  14. Merz U (2000a) Potato production and powdery scab situation in Switzerland. Proceedings of the First European Powdery Scab Workshop (pp 15–17) SAC, Aberdeen, Scotland (www.pa.ipw.agrl.ethz.ch/spongospora/EUworkshop/proceedings.html).Google Scholar
  15. Merz, U 2000bPowdery scab control in SwitzerlandSACAberdeen, Scotland, UK4344Proceedings of the First European Powdery Scab WorkshopGoogle Scholar
  16. Miyajima, K, Tanaka, F, Takeuchi, T, Kuninaga, S 1998Streptomyces turgidiscabies sp. novInternational Journal of Systematic Bacteriology48495502PubMedGoogle Scholar
  17. Nolasco, G, Blas, C, Torres, V, Ponz, F 1993A method combining immunocapture and PCR amplification in a microtiter plate for the detection of plant viruses and subviral pathogensJournal of Virological Methods45201218PubMedGoogle Scholar
  18. Pitts, T 2000Summary of the session of present control measuresSACAberdeen, Scotland, UK3133Proceedings of the First European Powdery Scab WorkshopGoogle Scholar
  19. Pridham, TG, Gottlieb, D 1948The utilisation of carbon compounds by some Actinomycetales as an aid for species determinationJournal of Bacteriology56107114Google Scholar
  20. Qu XS, Kavanagh JA and Egan D (1998). Development of specific and sensitive primers for the identification of Spongospora subterranea f.sp. subterranea. Faculty of Agriculture, University College, Dublin, National University of Ireland. (p. 206) Research Report 1996–1997.Google Scholar
  21. Qu XS, Kavanagh JA and Egan D (2000). Quantification of the fungal cause of potato powdery scab in soil using a competitive polymerase chain reaction assay (pp 74–76) Faculty of Agriculture, University College, Dublin, National University of Ireland. Research Report 1998–1999.Google Scholar
  22. Rolot, JL, Seutin, H 1999Soilless production of potato minitubers using a hydroponic techniquePotato Research42457469Google Scholar
  23. Graaf, P, Lees, AK, Cullen, DW, Duncan, JM 2003Detection and quantification of Spongospora subterranea in soil, water and plant tissue samples using real-time PCREuropean Journal of Plant Pathology109589597Google Scholar
  24. Wale, SJ, Burgess, PJ, Burnett, F 1993Bioassay for Spongospora subterranea detection in soil and its potential use in predicting powdery scab in the fieldMontrealCanada281Proceedings of 6th International Congress of Plant PathologyGoogle Scholar
  25. Wallace, A, Williams, NA, Lowe, R, Harrison, JG 1995Detection of Spongospora subterranea using monoclonal antibodies in ELISAPlant Pathology44355365Google Scholar
  26. Walsh, JA, Merz, U, Harrison, JG 1996Serological detection of spore balls of Spongospora subterranea and quantification in soilPlant Pathology45884895Google Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • U. Merz
    • 1
  • J. A. Walsh
    • 2
  • K. Bouchek-Mechiche
    • 3
  • Th. Oberhänsli
    • 4
  • W. Bitterlin
    • 4
  1. 1.Plant Sciences / Phytopathology GroupFITZurichSwitzerland
  2. 2.Warwick HRIWellesbourneUK
  3. 3.INRAUMR NiO3PLe RheuFrance
  4. 4.BIOREBA AGReinach BL1Switzerland

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