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European Journal of Plant Pathology

, Volume 111, Issue 1, pp 85–90 | Cite as

Identification and in planta detection of Pseudomonas syringae pv. tomato using PCR amplification of hrpZ Pst

  • Massimo Zaccardelli
  • Annalisa Spasiano
  • Carlo Bazzi
  • Massimo Merighi
Short communication

Abstract

A rapid detection method based on PCR amplification of Pseudomonas syringae pv. tomato chromosomal sequences was developed. Primer design was based on the P. syringae DC3000 hrpZ Pst gene, which maps on a pathogenicity-associated operon of the hrp/hrc pathogenicity island.

A 532 bp product corresponding to an internal fragment of hrpZ Pst was amplified from 50 isolates of P. syringae pv. tomato belonging to a geographically representative collection. The amplification product was also obtained from three coronatine-deficient strains of P. syringae pv. tomato.On the other hand, PCR did not produce any such products from 100 pathogenic and symbiotic bacterial strains of the genera Pseudomonas, Xanthomonas, Erwinia, and Rhizobium and 75 unidentified bacterial saprophytes isolated from tomato plants. The method was tested using leaf and fruit spots from naturally-infected tomato plants and asymptomatic nursery plants and artificially contaminated tomato seeds. The results confirmed the high specificity observed using pure cultures.

Keywords

diagnostics harpin Lycopersicon esculentum polymerase chain reaction 

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Copyright information

© Springer 2005

Authors and Affiliations

  • Massimo Zaccardelli
    • 1
  • Annalisa Spasiano
    • 1
  • Carlo Bazzi
    • 2
  • Massimo Merighi
    • 3
    • 4
  1. 1.Istituto Sperimentale per le Colture Industriali, Mi.P.A.F.Battipaglia (SA)Italy
  2. 2.Dipartimento di Scienze e Tecnologie Agroambientali (Di.S.T.A.), Patologia Vegetale, Alma Mater StudiorumUniversità di BolognaItaly
  3. 3.Department of Plant Pathology – Plant Molecular Biology/Biotechnology ProgramOhio State UniversityColumbusUSA
  4. 4.Department of Molecular Virology, Immunology and MedicanlGenetics/Center for Microbial Interface BiologyOhio State UniversityColumbusUSA

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