European Journal of Plant Pathology

, Volume 110, Issue 8, pp 823–831 | Cite as

Natural infections of tomato by Citrus exocortis viroid, Columnea latent viroid, Potato spindle tuber viroid and Tomato chlorotic dwarf viroid

  • J.th.j. Verhoeven
  • C.C.C. Jansen
  • T.M. Willemen
  • L.F.F. Kox
  • R.A. Owens
  • J.W. Roenhorst
Article

Abstract

Since 1988, viroids have been occasionally detected in samples of tomato (Lycopersicon esculentum) originating both in the Netherlands and other countries. Infected plants showed chlorosis, bronzing, leaf distortion and growth reduction. Initial diagnosis of these viroids was by return-polyacrylamide gel electrophoresis, which did not allow a further identification. This paper reports the identification of these viroids by reverse transcription-polymerase chain reaction and sequence analysis. Three known viroids of tomato, i.e. Citrus exocortis viroid, Potato spindle tuber viroid and Tomato chlorotic dwarf viroid were identified. In addition, six isolates were identified as Columnea latent viroid, a viroid so far only detected in some ornamental species. Like the isolates previously isolated from ornamental species, the isolates from tomato share genetic characteristics of both the genera Hostuviroid and Pospiviroid. The biological characteristics of all four viroids, especially their potential effects on both potato (Solanum tuberosum) and tomato, stress the need for reconsideration of their phytosanitary risks.

Hostuviroid Pospiviroid 

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References

  1. Altschul SF, Gish W, Miller W, Myers EW and Lipman DJ (1990)Basic local alignment search tool. Journal of Molecular Biology 215:403-410.Google Scholar
  2. Behjatnia SAA, Dry IB, Krake LR, Condé BD, Connelly MI, Randles JW and Rezaian MA (1996)New potato spindle tuber viroid and tomato leaf curl geminivirus strains from a wild Solanum sp. Phytopathology 86:880-886.Google Scholar
  3. Diener TO and Raymer WB (1971)Potato spindle tuber 'virus '. CMI/AAB Descriptions of Plant Viruses No. 66:4pp.Google Scholar
  4. Elliott DR, Alexander BJR, Smales TE, Tang Z and Clover GRG (2001)First report of Potato spindle tuber viroid in tomato in New Zealand. Plant Disease 85:1027.Google Scholar
  5. Fagoaga C and Duran-Vila N (1996)Naturally occurring variants of citrus exocortis viroid in vegetable crops. Plant Pathology 45:45-53.Google Scholar
  6. Felsenstein J (1987)Confidence limits on phylogenies:an approach using the bootstrap. Evolution 39:783-791.Google Scholar
  7. Flores R, Randles JW, Bar-Joseph M and Diener TO (2000) Viroids. In:van Regenmortel MHV, Fauquet CM, Bishop DHL, Carstens EB, Estes MK, Lemon SM, Maniloff J, Mayo MA, McGeoch DJ, Pringle CR and Wickner RB (eds) Virus Taxonomy. Seventh Report of the International Committee on Taxonomy of Viruses (pp 1009-1024)Academic Press, San Diego, USA.Google Scholar
  8. Galindo J, Smith DR and Diener TO (1982)Etiology of planta macho, a viroid disease of tomato. Phytopatology 72:49-54.Google Scholar
  9. Hammond R, Smith DR and Diener TO (1989)Nucleotide sequence and proposed secondary structure of Columnea latent viroid:A natural mosaic of viroid sequences. Nucleic Acids Research 17:10083-10094.Google Scholar
  10. Hooftman R, Arts MJ, Shamloul AM, Van Zaayen A and Hadidi A (1996)Detection of chrysanthemum stunt viroid by reverse transcription-polymerase chain reaction and by tissue blot hybridization. Acta Horticulturae 432:88-94.Google Scholar
  11. Huson DH (1998)SplitsTree:Analyzing and visualizing evolutionary data. Bioinformatics 14:68-73.Google Scholar
  12. Huttinga H, Mosch WHM and Treur A (1987)Comparison of bi-directional electrophoresis and molecular hybridisation methods to detect potato spindle tuber viroid and chrysan-themum stunt viroid. Bulletin OEPP/EPPO Bulletin 17:37-43.Google Scholar
  13. Leontyeva JA (1980)Tomatoes and potato spindle tuber viroid. Zashchita-Rastenii 8:22.Google Scholar
  14. Mackie AE, McKirdy SJ, Rodoni B and Kumar S (2002) Potato spindle tuber viroid eradicated in Western Australia. Australasian Plant Pathology 31:311-312.Google Scholar
  15. Manzer FE and Merriam D (1961)Field transmission of the potato spindle tuber virus and virus X by cultivating and hilling equipment. American Potato Journal 38:346-352.Google Scholar
  16. Martin WH (1922)''Spindle tuber '', a new potato trouble. Hints to potato growers, N. J. State Potato Association 3, no 8, USA.Google Scholar
  17. Mishra MD, Hammond RW, Owens RA, Smith DR and Diener TO (1991)Indian bunchy top disease of tomato plants is caused by a distinct strain of citrus exocortis viroid. Journal of General Virology 72:1781-1785.Google Scholar
  18. Mumford RA, Jarvis B and Skelton A (2003)The rst report of Potato spindle tuber viroid (PSTVd)in commercial tomatoes in the UK. New Disease Reports. [http://www.bspp.org.uk/ ndr ]8:August 2003-January 2004.Google Scholar
  19. Önelge N (1997)Direct nucleotide sequencing of citrus exocortis viroid (CEV). Turkish Journal of Agriculture and Forestry 21:419-422.Google Scholar
  20. Pfannenstiel MA and Slack SA (1980)Response of potato cultivars to infection by the potato spindle tuber viroid. Phytopathology 70:922-926.Google Scholar
  21. Puchta H, Herold T, Verhoeven K, Roenhorst A, Ramm K, Schmidt-Puchta W and Sänger HL (1990)A new strain of potato spindle tuber viroid (PSTVd-N)exhibits major sequence differences as compared to all other PSTVd strains sequenced so far. Plant Molecular Biology 15:509-511.Google Scholar
  22. Querci M, Owens RA, Vargas C and Salazar LF (1995) Detection of potato spindle tuber viroid in avocado growing in Peru. Plant Disease 79:196-202.Google Scholar
  23. Roenhorst JW, Butôt RPT, Van der Heijden KA, Hooftman M and Van Zaayen A (2000)Detection of chrysanthemum stunt viroid and potato spindle tuber viroid by return-polyacryl-amide gel electrophoresis. EPPO Bulletin 30:453-456.Google Scholar
  24. Semancik JS, Magnuson DS and Weathers LG (1973)Potato spindle tuber disease produced by pathogenic RNA from citrus exocortis disease:Evidence for the identity of the causal agents. Virology 52:292-294.Google Scholar
  25. Shamloul AM, Hadidi A, Zhu SF, Singh RP and Sagredo B (1997)Sensitive detection of potato spindle tuber viroid using RT-PCR and identi cation of a viroid variant naturally infecting pepino plants. Canadian Journal of Plant Pathology 19:89-96.Google Scholar
  26. Singh RP (1973)Experimental host range of the potato spindle tuber 'virus '. American Potato Journal 50:111-123.Google Scholar
  27. Singh RP, Lakshman DK, Boucher A and Tavantzis SM (1992) A viroid from Nematanthus wettsteinii plants closely related to the Columnea latent viroid. Journal of General Virology 73:2769-2774.Google Scholar
  28. Singh RP, Xianzhou N and Singh M (1999)Tomato chlorotic dwarf viroid:An evolutionary link in the origin of pospi-viroids. Journal of General Virology 80:2823-2828.Google Scholar
  29. Spieker RL (1996)A viroid from Brunfelsia undulata closely related to the Columnea latent viroid. Archives of Virology 141:1823-1832.Google Scholar
  30. Swofford DL (1993)Phylogenetic analysis using parsimony (PAUP), Version 4.0.0. University of Illinois, Champaign, IL.Google Scholar
  31. Thompson JD, Higgins DG and Gibson TJ (1994)CLUSTAL W:improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acids Research 22:4673-4680.Google Scholar
  32. Walter B (1987)Tomato apical stunt. In:Diener TO (ed)The Viroids. Plenum Press, New York, USA.Google Scholar
  33. Walter B, Thouvenal JC and Fauquet C (1980)Les viruses de la tomate en Cote d 'Ivore. Annales de Phytopathologie 12:259-275.Google Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • J.th.j. Verhoeven
    • 1
  • C.C.C. Jansen
    • 1
  • T.M. Willemen
    • 1
  • L.F.F. Kox
    • 1
  • R.A. Owens
    • 2
  • J.W. Roenhorst
    • 1
  1. 1.Plant Protection ServiceThe Netherlands
  2. 2.Molecular Plant Pathology LaboratoryPlant Sciences Institute, USDA/ARSUSA

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