European Journal of Plant Pathology

, Volume 111, Issue 2, pp 139–146

Expressed sequence tags from the phytopathogenic fungus Botrytis cinerea

  • Muriel Viaud
  • Fabrice Legeai
  • Jean-Marc Pradier
  • Yves Brygoo
  • Frédérique Bitton
  • Jean Weissenbach
  • Adeline Brunet-Simon
  • Aymeric Duclert
  • Sabine Fillinger
  • Dominique Fortini
  • Anastasia Gioti
  • Corinne Giraud
  • Sébastien Halary
  • Isabelle Lebrun
  • Pascal Le Pêcheur
  • Delphine Samson
  • Caroline Levis
Article

Abstract

A large set of genes was identified in the phytopathogenic fungus Botrytis cinerea by using an expressed sequence tag approach. The fungus was grown in axenic culture and a cDNA library was produced. From this library, 6559 ESTs were obtained. The combined sequences represent 3026 unisequences that corresponds to approximately one-quarter of the estimated total number of genes in B. cinerea. Approximately 18% of the ESTs showed significant similarities with genes coding for proteins with known functions,~56% were similar to genes coding for proteins with unknown functions and ~26% were orphans. A substantial B. cinerea gene inventory including putative virulence factors was therefore obtained and is now available at the Génoplante-Info Database interface (http://urgi.infobiogen.fr///Projects/GPiDB/Interface/).

Keywords

cDNA EST grey mould 

Abbreviations

CWDEs

cell wall degrading enzymes

ESTs

expressed sequence tags

GPiDB

Génoplante-Info DataBase.

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Copyright information

© Springer 2005

Authors and Affiliations

  • Muriel Viaud
    • 1
  • Fabrice Legeai
    • 2
  • Jean-Marc Pradier
    • 1
  • Yves Brygoo
    • 1
  • Frédérique Bitton
    • 1
    • 5
  • Jean Weissenbach
    • 3
  • Adeline Brunet-Simon
    • 1
  • Aymeric Duclert
    • 2
  • Sabine Fillinger
    • 4
  • Dominique Fortini
    • 1
  • Anastasia Gioti
    • 1
  • Corinne Giraud
    • 1
  • Sébastien Halary
    • 2
  • Isabelle Lebrun
    • 1
  • Pascal Le Pêcheur
    • 1
  • Delphine Samson
    • 2
  • Caroline Levis
    • 1
  1. 1.Unité PMDV, INRA, Route de Saint-CyrVersaillesFrance
  2. 2.URGI, INRA, InfobiogenEvryFrance
  3. 3.Génoscope, UMR CNRS 8030EvryFrance
  4. 4.UPMC, INRA, Route de Saint-CyrVersaillesFrance
  5. 5.URGV, INRAEvryFrance

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