Health risk assessment of heavy metal accumulation in the Buriganga and Turag River systems for Puntius ticto, Heteropneustes fossilis, and Channa punctatus

  • Mohammad Abdul BakiEmail author
  • Md Fajlul Haque Shojib
  • Subrina Sehrin
  • Sanjay Chakraborty
  • Tasrina Rabia Choudhury
  • Moumita Saha Bristy
  • Md. Kawser Ahmed
  • Sumiani Binti Yusoff
  • Md Firoz Khan
Original Paper


This study aimed to assess the effects of major ecotoxic heavy metals accumulated in the Buriganga and Turag River systems on the liver, kidney, intestine, and muscle of common edible fish species Puntius ticto, Heteropneustes fossilis, and Channa punctatus and determine the associated health risks. K was the predominant and reported as a major element. A large concentration of Zn was detected in diverse organs of the three edible fishes compared with other metals. Overall, trace metal analysis indicated that all organs (especially the liver and kidney) were under extreme threat because the maximum permissible limit set by different international health organizations was exceeded. The target hazard quotient and target cancer risk due to the trace metal content were the largest for P. ticto. Thus, excessive intake of P. ticto from the rivers Buriganga and Turag could result in chronic risks associated with long-term exposure to contaminants. Histopathological investigations revealed the first detectable indicators of infection and findings of long-term injury in cells, tissues, and organs. Histopathological changes in various tissue structures of fish functioned as key pointers of connection to pollutants, and definite infections and lesion types were established based on biotic pointers of toxic/carcinogenic effects. The analysis of histopathological alterations is a controlling integrative device used to assess pollutants in the environment.


Fish tissue Water pollution Bioaccumulation Histopathology Health risk 



The authors would like to thank the authority of the Atomic Energy Center, Shahbag, and pathology laboratory, BSMMU, Dhaka, Bangladesh. The authors also would like to thank the University of Malaya in Malaysia for Research University Grant IOES: TU001-2018. The authors also extend their thanks to Md Shahidur Rahmran Khan, Md. Azizul Maksud, and Lutfun Nahar, Atomic Energy Center, Md. Faruq (BSMMU, Dhaka, Bangladesh), and Muna (MPhil student of the Department of Zoology, Jagannath University, Dhaka, Bangladesh).

Supplementary material

10653_2019_386_MOESM1_ESM.docx (1.2 mb)
Supplementary material 1 (DOCX 1276 kb)


  1. Ahmad, M., Islam, S., Rahman, M., Haque, M., & Islam, M. (2010). Heavy metals in water, sediment and some fishes of Buriganga River, Bangladesh. International Journal of Environmental Research, 4, 321–332.Google Scholar
  2. Ahmed, M. K., Baki, M. A., Islam, M. S., Kundu, G. K., Habibullah-Al-Mamun, M., Sarkar, S. K., et al. (2015). Human health risk assessment of heavy metals in tropical fish and shellfish collected from the river Buriganga, Bangladesh. Environmental Science and Pollution Research, 22, 15880–15890. Scholar
  3. Alam, K. (2003). Cleanup of the Buriganga river: Integrating the environment into decision making. Ph.D. dissertation, Murdoch University.Google Scholar
  4. Ali, M. Y., Amin, M. N., & Alam, K. (2009). Ecological health risk of Buriganga river, Dhaka, Bangladesh. Hydro Nepal: Journal of Water, Energy and Environment, 3, 25–28.CrossRefGoogle Scholar
  5. Andreji, J., Dvorak, P., Dvorakova Liskova, Z., Massányi, P., Stranai, I., Nad, P., et al. (2012). Content of selected metals in muscle of cyprinid fish species from the Nitra River, Slovakia. Neuroendocrinology Letters, 33, 84–89.Google Scholar
  6. Annune, P., & Iyaniwura, T. (1994). Accumulation of two trace metals in tissues of freshwater fishes, Oreochoromis niloticus and Clarias gariepinus. Journal of Aquatic Food Product Technology, 2, 5–18.CrossRefGoogle Scholar
  7. Barone, G., Giacominelli-Stuffler, R., & Storelli, M. M. (2013). Comparative study on trace metal accumulation in the liver of two fish species (Torpedinidae): Concentration–size relationship. Ecotoxicology and Environmental Safety, 97, 73–77. Scholar
  8. BBS. (2011). Bangladesh: Household income and expenditure survey. Bangladesh: Bangladesh Bureau of Statistics.Google Scholar
  9. Bhupander, K., & Mukherjee, D. (2011). Assessment of human health risk for arsenic, copper, nickel, mercury and zinc in fish collected from tropical wetlands in India. Advances in Life Science and Technology, 2, 13–24.Google Scholar
  10. Das, B. K., & Mukherjee, S. C. (2000). A histopathological study of carp (Labeo rohita) exposed to hexachlorocyclohexane. Veterinarski Arhiv, 70, 169–180.Google Scholar
  11. Dassenakis, M., Scoullos, M., Foufa, E., Krasakopoulou, E., Pavlidou, A., & Kloukiniotou, M. (1998). Effects of multiple source pollution on a small Mediterranean river. Applied Geochemistry, 13, 197–211. Scholar
  12. Fryer, M., Collins, C. D., Ferrier, H., Colvile, R. N., & Nieuwenhuijsen, M. J. (2006). Human exposure modelling for chemical risk assessment: A review of current approaches and research and policy implications. Environmental Science & Policy, 9, 261–274. Scholar
  13. Gbem, T. T., Balogun, J. K., Lawal, F. A., & Annune, P. A. (2001). Trace metal accumulation in Clarias gariepinus (Teugels) exposed to sublethal levels of tannery effluent. Science of the Total Environment, 271, 1–9. Scholar
  14. Harmanescu, M., Alda, L. M., Bordean, D. M., Gogoasa, I., & Gergen, I. (2011). Heavy metals health risk assessment for population via consumption of vegetables grown in old mining area; a case study: Banat County, Romania. Chemistry Central Journal, 5, 64. Scholar
  15. Heath, A. G. (1995). Water pollution and fish physiology. Boca Raton: CRC Press.Google Scholar
  16. Jovičić, K., et al. (2015). Mapping differential elemental accumulation in fish tissues: assessment of metal and trace element concentrations in wels catfish (Silurus glanis) from the Danube River by ICP-MS. Environmental Science and Pollution Research, 22, 3820–3827. Scholar
  17. Kawser Ahmed, M., Baki, M. A., Kundu, G. K., Saiful Islam, M., Monirul Islam, M., & Muzammel Hossain, M. (2016). Human health risks from heavy metals in fish of Buriganga river, Bangladesh. SpringerPlus, 5, 1697. Scholar
  18. Kranz, H., & Peters, N. (1985). Pathological conditions in the liver of ruffe, Gymnocephalus cernua (L.), from the Elbe estuary. Journal of Fish Diseases, 8, 13–24.CrossRefGoogle Scholar
  19. Kundu, G. K., et al. (2017). Metal contamination of commercial fish feed and quality aspects of farmed tilapia (Oreochromis niloticus) in Bangladesh. Bioresearch Communications, 3, 345–353.Google Scholar
  20. Lang, T. (2002). Fish disease surveys in environmental monitoring: The role of ICES. In ICES marine science symposia (pp. 202–212).Google Scholar
  21. Langston, W. (1990). Toxic effects of metals and the incidence of marine ecosystems. In R. W. Furness & P. S. Rainbow (Eds.), Heavy metals in the marine environment. RW Furness, PS Rainbow. New York: CRC Press.Google Scholar
  22. Li, J., Huang, Z. Y., Hu, Y., & Yang, H. (2013). Potential risk assessment of heavy metals by consuming shellfish collected from Xiamen, China. Environmental Science and Pollution Research, 20, 2937–2947. Scholar
  23. Miller, C. V., Foster, G. D., & Majedi, B. F. (2003). Baseflow and stormflow metal fluxes from two small agricultural catchments in the Coastal Plain of the Chesapeake Bay Basin, United States. Applied Geochemistry, 18, 483–501. Scholar
  24. Minier, C., Abarnou, A., Jaouen-Madoulet, A., Guellec, A. M. L., Tutundjian, R., Bocquené, G., et al. (2006). A pollution monitoring pilot study involving contaminant and biomarker measurements in the Seine Estuary, France, using zebra mussels (Dreissena polymorpha). Environmental Toxicology and Chemistry, 25, 112–119. Scholar
  25. Miranda, A. L., Roche, H., Randi, M. A. F., Menezes, M. L., & Ribeiro, C. A. O. (2008). Bioaccumulation of chlorinated pesticides and PCBs in the tropical freshwater fish Hoplias malabaricus: Histopathological, physiological, and immunological findings. Environment International, 34, 939–949. Scholar
  26. Mohamed, F. A. (2009). Histopathological studies on Tilapia zillii and Solea vulgaris from Lake Qarun, Egypt. World Journal of Fish and Marine Sciences, 1, 29–39.Google Scholar
  27. Mohiuddin, K., Alam, M., Ahmed, I., & Chowdhury, A. (2016). Heavy metal pollution load in sediment samples of the Buriganga river in Bangladesh. Journal of the Bangladesh Agricultural University, 13, 229–238.CrossRefGoogle Scholar
  28. Moniruzzaman, M., Elahi, S. F., & Jahangir, M. A. A. (2009). Study on temporal variation of physico-chemical parameters of Buriganga river water through GIS (Geographical Information System) Technology Bangladesh. Journal of Scientific & Industrial Research, 44, 327–334.Google Scholar
  29. Myers, M. S., & Fournie, J. W. (2002). Histopathological biomarkers as integrators of anthropogenic and environmental stressors. In Biological indicators of aquatic ecosystem stress (pp. 221–287).Google Scholar
  30. Pacheco, M., & Santos, M. A. (2002). Biotransformation, genotoxic, and histopathological effects of environmental contaminants in European eel (Anguilla anguilla L.). Ecotoxicology and Environmental Safety, 53, 331–347. Scholar
  31. Paris-Palacios, S., Biagianti-Risbourg, S., & Vernet, G. (2000). Biochemical and (ultra)structural hepatic perturbations of Brachydanio rerio (Teleostei, Cyprinidae) exposed to two sublethal concentrations of copper sulfate. Aquatic Toxicology, 50, 109–124. Scholar
  32. Rao, L., & Padmaja, G. (2000). Bioaccumulation of heavy metals in M. cyprinoids from the harbor waters of Visakhapatnam. Bulletin of Pure and Applied Sciences, 19, 77–85.Google Scholar
  33. Reimschuessel, R. (2001). A fish model of renal regeneration and development. ILAR Journal, 42, 285–291.CrossRefGoogle Scholar
  34. Ribeiro, C. A. O., Vollaire, Y., Sanchez-Chardi, A., & Roche, H. (2005). Bioaccumulation and the effects of organochlorine pesticides, PAH and heavy metals in the Eel (Anguilla anguilla) at the Camargue Nature Reserve. France Aquatic Toxicology, 74, 53–69. Scholar
  35. Roberts, R. J. (1978). Fish pathology. London: Bailliere Tindall.Google Scholar
  36. Rodrigues, E. L., & Fanta, E. (1998). Liver histopathology of the fish Brachydanio rerio after acute exposure to sublethal levels of the organophosphate Dimetoato 500. Revista Brasileira de Zoologia, 15, 441–450.CrossRefGoogle Scholar
  37. Saha, P. K., & Hossain, M. (2011). Assessment of heavy metal contamination and sediment quality in the Buriganga River, Bangladesh. In 2nd international conference on environmental science and technology, IPCBEE, Singapore, 2011.Google Scholar
  38. Schwaiger, J., Wanke, R., Adam, S., Pawert, M., Honnen, W., & Triebskorn, R. (1997). The use of histopathological indicators to evaluate contaminant-related stress in fish. Journal of Aquatic Ecosystem Stress and Recovery, 6, 75–86. Scholar
  39. Siscar, R., Torreblanca, A., del Ramo, J., & Solé, M. (2014). Modulation of metallothionein and metal partitioning in liver and kidney of Solea senegalensis after long-term acclimation to two environmental temperatures. Environmental Research, 132, 197–205. Scholar
  40. Svobodova, Z., et al. (1996). Evaluation of the effect of chemical substances, preparations, wastes and waste waters on organisms in the aquatic environment. Bulletin Vyzkumneho Ustavu Rybarskeho a Hydrobiologickeho Jihoceske Univerzity Vodnany (Czech Republic), 32, 76–96.Google Scholar
  41. Taweel, A., Shuhaimi-Othman, M., & Ahmad, A. K. (2013). Assessment of heavy metals in tilapia fish (Oreochromis niloticus) from the Langat River and Engineering Lake in Bangi, Malaysia, and evaluation of the health risk from tilapia consumption. Ecotoxicology and Environmental Safety, 93, 45–51. Scholar
  42. Teh, S. J., Adams, S. M., & Hinton, D. E. (1997). Histopathologic biomarkers in feral freshwater fish populations exposed to different types of contaminant stress. Aquatic Toxicology, 37, 51–70. Scholar
  43. Thophon, S., Kruatrachue, M., Upatham, E. S., Pokethitiyook, P., Sahaphong, S., & Jaritkhuan, S. (2003). Histopathological alterations of white seabass, Lates calcarifer, in acute and subchronic cadmium exposure. Environmental Pollution, 121, 307–320. Scholar
  44. USEPA. (1989). Guidance manual for assessing human health risks from chemically contaminated, fish and shellfish. EPA-503/8-89-002. USEPA, Washington, DC.Google Scholar
  45. USEPA. (2010). Risk-based concentration table. Accessed 25 Nov 2018.
  46. USEPA. (2011). USEPA regional screening level (RSL) summary table: November 2011.
  47. Wester, P. W., & Canton, J. H. (1991). The usefulness of histopathology in aquatic toxicity studies. Comparative Biochemistry and Physiology Part C: Comparative Pharmacology, 100, 115–117. Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Mohammad Abdul Baki
    • 1
    Email author
  • Md Fajlul Haque Shojib
    • 1
  • Subrina Sehrin
    • 1
  • Sanjay Chakraborty
    • 1
  • Tasrina Rabia Choudhury
    • 2
  • Moumita Saha Bristy
    • 1
  • Md. Kawser Ahmed
    • 3
  • Sumiani Binti Yusoff
    • 4
  • Md Firoz Khan
    • 4
    • 5
  1. 1.Department of Zoology, Faculty of Life and Earth ScienceJagannath UniversityDhakaBangladesh
  2. 2.Analytical Chemistry Laboratory, Atomic Energy Center DhakaBangladesh Atomic Energy CommissionDhakaBangladesh
  3. 3.Department of OceanographyUniversity of DhakaDhakaBangladesh
  4. 4.Institute of Ocean and Earth Sciences (IOES)University of MalayaKuala LumpurMalaysia
  5. 5.Department of Chemistry, Faculty of ScienceUniversity of MalayaKuala LumpurMalaysia

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