Environmental Geochemistry and Health

, Volume 40, Issue 6, pp 2773–2784 | Cite as

Toxicity effects and biomarkers of tebufenozide exposure in Yuukianura szeptyckii (Collembola: Neanuridae)

  • Yun-Sik Lee
  • Sung-Eun Lee
  • Jino Son
  • Yongeun Kim
  • June Wee
  • Kijong Cho
Original Paper


Tebufenozide is an insect growth regulator used to control pest caterpillar populations. As an ecdysone agonist, tebufenozide is equally toxic to several non-target arthropod species, binding the receptor sites of the molting hormone 20-hydroxyecdysone and causing premature and lethal molting. In this study, the toxic effects of tebufenozide were assessed, and biomarkers of tebufenozide exposure were identified, in the non-target soil collembolan species Yuukianura szeptyckii. Adult mortality and reproduction in Y. szeptyckii exposed to tebufenozide were evaluated after 28 days of exposure and were used to calculate LC50 and EC50, respectively. The LC50 could not be determined, because the mortality values observed were below 50%, even when exposed to the highest concentration tested (700 mg/kg), but the EC50 was 95.5 mg/kg. Effects on hatching and molting rates were evaluated using compressed soils, to prevent experimental individuals from burrowing; thus, all eggs and exuviae were detectable on the soil surface. Significant negative effects of tebufenozide exposure on the hatching rate and molting frequency were observed only at the highest concentration tested (700 mg/kg). Proteomic analyses were conducted to detect the cryptic effects of toxicity in adult collembolans exposed for 28 days to 43.8 mg/kg of tebufenozide, a concentration at which no toxicity effects were observed. The production rates of two ribosomal proteins, as well as proteins involved in apoptotic cell signaling, were higher in collembolans exposed to tebufenozide than in the control group. However, the production of proteins involved in glycolysis and energy production was downregulated. Therefore, the ecotoxicoproteomic approach is a promising tool for measuring the cryptic effects of tebufenozide exposure in Y. szeptyckii at low concentrations.


Non-target species Reproduction Molting Hatching Ecotoxicoproteomic approach 



This subject is supported by Korea Ministry of Environment (MOE) as “Chemical Accident Prevention and Response Technology Development Project” (2016001970003) and National Research Foundation of Korea as “Prediction of soil invertebrate habitat fitness under climate change using fuzzy rule based model” (NRF-2016R1A2B4015651).


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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Yun-Sik Lee
    • 1
  • Sung-Eun Lee
    • 2
  • Jino Son
    • 1
  • Yongeun Kim
    • 1
  • June Wee
    • 1
  • Kijong Cho
    • 1
  1. 1.Division of Environmental Science and Ecological EngineeringKorea UniversitySeoulRepublic of Korea
  2. 2.School of Applied BiosciencesKyungpook National UniversityDaeguRepublic of Korea

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