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Environmental Geochemistry and Health

, Volume 40, Issue 5, pp 2191–2203 | Cite as

Antibiotic distribution, risk assessment, and microbial diversity in river water and sediment in Hong Kong

  • Wen-Jing Deng
  • Na Li
  • Guang-Guo Ying
Original Paper
  • 235 Downloads

Abstract

For the past fewer years, environment antibiotic residues have got more and more attention. The occurrence and distribution of eight common antibiotics, belonging to five classes, were determined in both water and sediment of eleven rivers of Hong Kong. The target antibiotics were found to be widely distributed. Sulfamethoxazole (n.d.–79.9 ng/L), sulfadimidine (n.d.–29.9 ng/L), and ofloxacin (n.d.–75.5 ng/L) were the dominant antibiotics in river water, with detection rates of 84.6, 76.9, and 69.2%, respectively. Tetracycline (n.d.–9.8 ng/g) was the dominant antibiotic in sediment, with a detection rate of 60%. The concentrations of all antibiotics in river water of Hong Kong were lower than which in various rivers of Europe, North America and Australia, as well as the Pearl River Basin of China. All sediment sites exhibited significant bacterial diversity. Gammaproteobacteria (0.08–12.7%) and Flavobacteria (0.14–14.1%) were the dominant bacterial classes in all sediments. The bacterial compositions varied between sites; areas polluted with high levels of antibiotics had rich and highly diverse bacterial communities. The environmental risk assessment determined that the antibiotics in 73.1% of the samples posed ecological risks to algae, and two samples posed low risks to invertebrates. Ofloxacin was the main contributor of risk to aquatic organisms, while the antibiotics in 11.5% of the samples posed resistance selection risks.

Graphical Abstract

The occurrence and distribution of eight common antibiotics, belonging to five classes, were widely distributed in Hong Kong. Sulfamethoxazole, sulfadimidine, and ofloxacin were the dominant antibiotics in river waters, Tetracycline was the dominant antibiotic in sediment. Areas polluted with high levels of antibiotics had rich and highly diverse bacterial communities. Antibiotics in 73.1% of the samples posed ecological risks, while the antibiotics in 11.5% of the samples posed resistance selection risks.

Keywords

Antibiotics Rivers Microbial community Risk assessment UPLC-ES-MS/MS 

Notes

Acknowledgements

The authors acknowledge the support of the Early Career Start/General Research Fund of Hong Kong (Code No. ECS/GRF 845212), FLASS Dean’s Research Fund (Ref. No. 04200), Internal Research Grant of the Education University of Hong Kong (Ref. No. R3807, R3919). The project was supported by National Natural Science Foundation of China (Grant No. U1701242).

Supplementary material

10653_2018_92_MOESM1_ESM.docx (1.2 mb)
Supplementary material 1 (DOCX 1273 kb)

References

  1. Andreozzi, R., Raffaele, M., & Nicklas, P. (2003). Pharmaceuticals in STP effluents and their solar photodegradation in aquatic environment. Chemosphere, 50, 1319–1330.CrossRefGoogle Scholar
  2. Bengtsson-Palme, J., & Larsson, D. G. J. (2016). Concentrations of antibiotics predicted to select for resistant bacteria: Proposed limits for environmental regulation. Environment International, 86, 140–149.CrossRefGoogle Scholar
  3. Bouki, C., Venieri, D., & Diamadopoulos, E. (2013). Detection and fate of antibiotic resistant bacteria in wastewater treatment plants: A review. Ecotoxicology and Environmental Safety, 91, 1–9.CrossRefGoogle Scholar
  4. Boxall, A. B. A., Kolpin, D. W., Halling-Sørensen, B., & Tolls, J. (2003). Peer reviewed: Are veterinary medicines causing environmental risks? Environmental Science and Technology, 37, 286A–294A.CrossRefGoogle Scholar
  5. Bushaw-Newton, K. L., Ewers, E. C., Velinsky, D. J., Ashley, J. T. F., & MacAvoy, S. E. (2012). Bacterial community profiles from sediments of the Anacostia River using metabolic and molecular analyses. Environmental Science and Pollution Research, 19, 1271–1279.CrossRefGoogle Scholar
  6. Caporaso, J. G., Bittinger, K., Bushman, F. D., DeSantis, T. Z., Andersen, G. L., & Knight, R. (2009). PyNAST: A flexible tool for aligning sequences to a template alignment. Bioinformatics, 26, 266–267.CrossRefGoogle Scholar
  7. Caporaso, J. G., Kuczynski, J., Stombaugh, J., Bittinger, K., Bushman, F. D., et al. (2010). QIIME allows analysis of high-throughput community sequencing data. Nature Methods, 7, 335–336.CrossRefGoogle Scholar
  8. Chen, K., & Zhou, J. L. (2014). Occurrence and behavior of antibiotics in water and sediments from the Huangpu River, Shanghai, China. Chemosphere, 95, 604–612.CrossRefGoogle Scholar
  9. Daughton, C. G., & Ternes, T. A. (1999). Pharmaceuticals and personal care products in the environment: Agents of subtle change? Environmental Health Perspectives, 107, 907–938.CrossRefGoogle Scholar
  10. Deng, W., Li, N., Zheng, H., & Lin, H. (2016). Occurrence and risk assessment of antibiotics in river water in Hong Kong. Ecotoxicology and Environmental Safety, 125, 121–127.CrossRefGoogle Scholar
  11. DeSantis, T. Z., Hugenholtz, P., Larsen, N., Rojas, M., Brodie, E. L., Keller, K., et al. (2006). Greengenes, a chimera-checked 16S rRNA gene database and workbench compatible with ARB. Applied and Environmental Microbiology, 72, 5069–5072.CrossRefGoogle Scholar
  12. Dethlefsen, L., & Relman, D. A. (2011). Incomplete recovery and individualized responses of the human distal gut microbiota to repeated antibiotic perturbation. Proceedings of the National Academy of Sciences, 108, 4554–4561.CrossRefGoogle Scholar
  13. EC (European Commission). (2003). European commission Technical Guidance Document in Support of Commission Directive 93//67/EEC on Risk Assessment for New Notified Substances and Commission Regulation (EC) No. 1488/94 on Risk Assessment for Existing Substance, Part II. In: Commission, E. (Ed.). pp. 100–103.Google Scholar
  14. Gao, P., Mao, D., Luo, Y., Wang, L., Xu, B., & Xu, L. (2012). Occurrence of sulfonamide and tetracycline-resistant bacteria and resistance genes in aquaculture environment. Water Research, 46, 2355–2364.CrossRefGoogle Scholar
  15. García-Galán, M. J., Díaz-Cruz, M. S., & Barceló, D. (2011). Occurrence of sulfonamide residues along the Ebro river basin: Removal in wastewater treatment plants and environmental impact assessment. Environment International, 37, 462–473.CrossRefGoogle Scholar
  16. Gibbons, S. M., Jones, E., Bearquiver, A., Blackwolf, F., Roundstone, W., Scott, N., et al. (2014). Human and environmental impacts on river sediment microbial communities. PLoS ONE, 9, e97435.CrossRefGoogle Scholar
  17. Guerra, P., Kim, M., Shah, A., Alaee, M., & Smyth, S. A. (2014). Occurrence and fate of antibiotic, analgesic/anti-inflammatory, and antifungal compounds in five wastewater treatment processes. Science of the Total Environment, 473–474, 235–243.CrossRefGoogle Scholar
  18. Halling-Sørensen, B., Lützhøft, H.-C. H., Andersen, H. R., & Ingerslev, F. (2000). Environmental risk assessment of antibiotics: Comparison of mecillinam, trimethoprim and ciprofloxacin. Journal of Antimicrobial Chemotherapy, 46, 53–58.CrossRefGoogle Scholar
  19. Huang, Q., Zhang, K., Wang, Z., Wang, C., & Peng, X. (2012). Enantiomeric determination of azole antifungals in wastewater and sludge by liquid chromatography–tandem mass spectrometry. Analytical and Bioanalytical Chemistry, 403, 1751–1760.CrossRefGoogle Scholar
  20. Jakobsson, H. E., Jernberg, C., Andersson, A. F., Sjölund-Karlsson, M., Jansson, J. K., & Engstrand, L. (2010). Short-term antibiotic treatment has differing long-term impacts on the human throat and gut microbiome. PLoS ONE, 5, e9836.CrossRefGoogle Scholar
  21. Jernberg, C., Lofmark, S., Edlund, C., & Jansson, J. K. (2007). Long-term ecological impacts of antibiotic administration on the human intestinal microbiota. ISME Journal, 1, 56–66.CrossRefGoogle Scholar
  22. Jiang, L., Hu, X., Yin, D., Zhang, H., & Yu, Z. (2011). Occurrence, distribution and seasonal variation of antibiotics in the Huangpu River, Shanghai, China. Chemosphere, 82, 822–828.CrossRefGoogle Scholar
  23. Khetan, S. K., & Collins, T. J. (2007). Human pharmaceuticals in the aquatic environment: A challenge to green chemistry. Chemical Reviews, 107, 2319–2364.CrossRefGoogle Scholar
  24. Kohanski, M. A., DePristo, M. A., & Collins, J. J. (2010). Sublethal antibiotic treatment leads to multidrug resistance via radical-induced mutagenesis. Molecular Cell, 37, 311–320.CrossRefGoogle Scholar
  25. Kolpin, D. W., Furlong, E. T., Meyer, M. T., Thurman, E. M., Zaugg, S. D., Barber, L. B., et al. (2002). Pharmaceuticals, hormones, and other organic wastewater contaminants in U.S. streams, 1999–2000: A national reconnaissance. Environmental Science and Technology, 36, 1202–1211.CrossRefGoogle Scholar
  26. Kümmerer, K. (2009). Antibiotics in the aquatic environment—a review—Part I. Chemosphere, 75, 417–434.CrossRefGoogle Scholar
  27. Laxminarayan, R., Duse, A., Wattal, C., Zaidi, A. K. M., Wertheim, H. F. L., Sumpradit, N., et al. (2013). Antibiotic resistance—the need for global solutions. The Lancet Infectious Diseases, 13, 1057–1098.CrossRefGoogle Scholar
  28. Lee, Y.-J., Lee, S.-E., Lee, D. S., & Kim, Y.-H. (2008). Risk assessment of human antibiotics in Korean aquatic environment. Environmental Toxicology and Pharmacology, 26, 216–221.CrossRefGoogle Scholar
  29. Li, W. H., Gao, L. H., Shi, Y. L., Liu, J. M., & Cai, Y. Q. (2015). Occurrence, distribution and risks of antibiotics in urban surface water in Beijing, China. Environmental Science Processes & Impacts, 17, 1611–1619.CrossRefGoogle Scholar
  30. Lindberg, R. H., Fick, J., & Tysklind, M. (2010). Screening of antimycotics in Swedish sewage treatment plants—waters and sludge. Water Research, 44, 649–657.CrossRefGoogle Scholar
  31. Lozupone, C., & Knight, R. (2005). UniFrac: A new phylogenetic method for comparing microbial communities. Applied and Environmental Microbiology, 71, 8228–8235.CrossRefGoogle Scholar
  32. Minh, T. B., Leung, H. W., Loi, I. H., Chan, W. H., So, M. K., Mao, J. Q., et al. (2009). Antibiotics in the Hong Kong metropolitan area: Ubiquitous distribution and fate in Victoria Harbour. Marine Pollution Bulletin, 58, 1052–1062.CrossRefGoogle Scholar
  33. Pei, R., Kim, S.-C., Carlson, K. H., & Pruden, A. (2006). Effect of river landscape on the sediment concentrations of antibiotics and corresponding antibiotic resistance genes (ARG). Water Research, 40, 2427–2435.CrossRefGoogle Scholar
  34. Peng, X., Huang, Q., Zhang, K., Yu, Y., Wang, Z., & Wang, C. (2012). Distribution, behavior and fate of azole antifungals during mechanical, biological, and chemical treatments in sewage treatment plants in China. Science of the Total Environment, 426, 311–317.CrossRefGoogle Scholar
  35. Pouliquen, H., & Le Bris, H. (1996). Sorption of oxolinic acid and oxytetracycline to marine sediments. Chemosphere, 33, 801–815.CrossRefGoogle Scholar
  36. Price, M. N., Dehal, P. S., & Arkin, A. P. (2010). Fast tree 2—approximately maximum-likelihood trees for large alignments. PLoS ONE, 5, e9490.CrossRefGoogle Scholar
  37. Robinson, A. A., Belden, J. B., & Lydy, M. J. (2005). Toxicity of fluoroquinolone antibiotics to aquatic organisms. Environmental Toxicology and Chemistry, 24, 423–430.CrossRefGoogle Scholar
  38. Schloss, P. D., Westcott, S. L., Ryabin, T., Hall, J. R., Hartmann, M., Hollister, E. B., et al. (2009). Introducing mothur: Open-source, platform-independent, community-supported software for describing and comparing microbial communities. Applied and Environmental Microbiology, 75, 7537–7541.CrossRefGoogle Scholar
  39. Stoob, K., Singer, H. P., Mueller, S. R., Schwarzenbach, R. P., & Stamm, C. H. (2007). Dissipation and transport of veterinary sulfonamide antibiotics after manure application to grassland in a small catchment. Environmental Science and Technology, 41, 7349–7355.CrossRefGoogle Scholar
  40. Sun, J., Luo, Q., Wang, D., & Wang, Z. (2015). Occurrences of pharmaceuticals in drinking water sources of major river watersheds, China. Ecotoxicology and Environmental Safety, 117, 132–140.CrossRefGoogle Scholar
  41. Tamtam, F., Mercier, F., Le Bot, B., Eurin, J., Tuc Dinh, Q., Clément, M., et al. (2008). Occurrence and fate of antibiotics in the Seine River in various hydrological conditions. Science of the Total Environment, 393, 84–95.CrossRefGoogle Scholar
  42. Tang, J., Shi, T. Z., Wu, X. W., Cao, H. Q., Li, X. D., Hua, R. M., et al. (2015). The occurrence and distribution of antibiotics in Lake Chaohu, China: Seasonal variation, potential source and risk assessment. Chemosphere, 122, 154–161.CrossRefGoogle Scholar
  43. Van Doorslaer, X., Dewulf, J., Van Langenhove, H., & Demeestere, K. (2014). Fluoroquinolone antibiotics: An emerging class of environmental micropollutants. Science of the Total Environment, 500–501, 250–269.CrossRefGoogle Scholar
  44. Wang, H., Wang, B., Zhao, Q., Zhao, Y., Fu, C., Feng, X., et al. (2015). Antibiotic body burden of Chinese School Children: A multisite biomonitoring-based study. Environmental Science and Technology, 49, 5070–5079.CrossRefGoogle Scholar
  45. Wang, Y., Sheng, H.-F., He, Y., Wu, J.-Y., Jiang, Y.-X., Tam, N. F.-Y., et al. (2012). Comparison of the levels of bacterial diversity in freshwater, intertidal wetland, and marine sediments by using millions of illumina tags. Applied and Environmental Microbiology, 78, 8264–8271.CrossRefGoogle Scholar
  46. Watkinson, A. J., Murby, E. J., Kolpin, D. W., & Costanzo, S. D. (2009). The occurrence of antibiotics in an urban watershed: From wastewater to drinking water. Science of the Total Environment, 407, 2711–2723.CrossRefGoogle Scholar
  47. Werner, J. J., Koren, O., Hugenholtz, P., DeSantis, T. Z., Walters, W. A., Caporaso, J. G., et al. (2012). Impact of training sets on classification of high-throughput bacterial 16 s rRNA gene surveys. ISME Journal, 6, 94–103.CrossRefGoogle Scholar
  48. Xu, W., Zhang, G., Zou, S., Li, X., & Liu, Y. (2007). Determination of selected antibiotics in the Victoria Harbour and the Pearl River, South China using high-performance liquid chromatography-electrospray ionization tandem mass spectrometry. Environmental Pollution, 145, 672–679.CrossRefGoogle Scholar
  49. Xue, B. M., Zhang, R. J., Wang, Y. H., Liu, X., Li, J., & Zhang, G. (2013). Antibiotic contamination in a typical developing city in south China: Occurrence and ecological risks in the Yongjiang River impacted by tributary discharge and anthropogenic activities. Ecotoxicology and Environmental Safety, 92, 229–236.CrossRefGoogle Scholar
  50. Yang, J. F., Ying, G. G., Zhao, J. L., Tao, R., Su, H. C., & Liu, Y. S. (2011). Spatial and seasonal distribution of selected antibiotics in surface waters of the Pearl Rivers, China. Journal of Environmental Science and Health, Part B, 46, 272–280.CrossRefGoogle Scholar
  51. Yiruhan, Y., Wang, Q. J., Mo, C. H., Li, Y. W., Gao, P., Tai, Y. P., et al. (2010). Determination of four fluoroquinolone antibiotics in tap water in Guangzhou and Macao. Environmental Pollution, 158, 2350–2358.CrossRefGoogle Scholar
  52. Zhang, Q. Q., Ying, G. G., Pan, C. G., Liu, Y. S., & Zhao, J.-L. (2015). Comprehensive evaluation of antibiotics emission and fate in the river basins of China: Source analysis, multimedia modeling, and linkage to bacterial resistance. Environmental Science and Technology, 49, 6772–6782.CrossRefGoogle Scholar
  53. Zhang, R., Zhang, G., Zheng, Q., Tang, J., Chen, Y., Xu, W., et al. (2012). Occurrence and risks of antibiotics in the Laizhou Bay, China: Impacts of river discharge. Ecotoxicology and Environmental Safety, 80, 208–215.CrossRefGoogle Scholar
  54. Zou, S., Xu, W., Zhang, R., Tang, J., Chen, Y., & Zhang, G. (2011). Occurrence and distribution of antibiotics in coastal water of the Bohai Bay, China: Impacts of river discharge and aquaculture activities. Environmental Pollution, 159, 2913–2920.CrossRefGoogle Scholar
  55. Zuccato, E., Castiglioni, S., Bagnati, R., Melis, M., & Fanelli, R. (2010). Source, occurrence and fate of antibiotics in the Italian aquatic environment. Journal of Hazardous Materials, 179, 1042–1048.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Science and Environmental StudiesThe Education University of Hong KongTai Po, N.T.Hong Kong
  2. 2.State Key Laboratory of Organic Geochemistry, Guangzhou Institute of GeochemistryChinese Academy of SciencesGuangzhouChina
  3. 3.The Environmental Research Institute, MOE Key Laboratory of Environmental Theoretical ChemistrySouth China Normal UniversityGuangzhouChina

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