Assessment of Pb, Cd and Hg soil contamination and its potential to cause cytotoxic and genotoxic effects in human cell lines (CaCo-2 and HaCaT)
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Soil contamination by heavy metals is a serious global environmental problem, especially for developing countries. A large number of industrial plants, which continually pollute the environment, characterize Tuzla Canton, Bosnia and Herzegovina. The aim of this study was to assess the level of soil pollution by heavy metals and to estimate cytotoxicity and genotoxicity of soil leachates from this area. Lead (Pb), cadmium (Cd) and mercury (Hg) were analyzed by ICP-AES and AAS. Soil contamination was assessed using contamination factor, degree of contamination, geoaccumulation index and pollution load index. To determine the connection of variables and understanding their origin in soils, principal component analysis (PCA) and cluster analysis (CA) were used. The results indicate that Cd and Hg originated from natural and anthropogenic activities, while Pb is of anthropogenic origin. For toxicity evaluation, CaCo-2 and HaCaT cells were used. PrestoBlue assay was used for cytotoxicity testing, and γH2A.X for genotoxicity evaluation. Concerning cytotoxicity, Cd and Hg had a positive correlation with cytotoxicity in HaCaT cells, but only Hg induced cytotoxicity in CaCo-2 cells. We also demonstrate that soil leachates contaminated by heavy metals can induce genotoxicity in both used cell lines. According to these results, combining bioassays with standard physicochemical analysis can be useful for evaluating environmental and health risks more accurately. These results are important for developing proper management strategies to decrease pollution. This is one of the first studies from this area and an important indication of soil quality in Southeast Europe.
KeywordsLead Cadmium and mercury Soil pollution Tuzla Canton Cytotoxicity Genotoxicity
This research was partly supported by project of Federal Ministry of Education and Science in the framework of bilateral scientific and technological cooperation between the Republic Slovenia and Bosnia and Herzegovina for 2016–2017 (BI-BA/16-17-036). Furthermore, the financial support of meat industry Natura from Teslic, Bosnia and Herzegovina is gratefully acknowledged.
- Alimba, C. G., Gandhi, D., Sivanesan, S., Bhanarkar, M. D., Naoghare, P. K., Bakare, A. A., et al. (2016). Chemical characterization of simulated landfill soil leachates from Nigeria and India and their cytotoxicity and DNA damage inductions on three human cell lines. Chemosphere, 164, 469–479.CrossRefGoogle Scholar
- ATSDR. (1999). Toxicological profile for mercury. Agency for Toxic Substances and Disease Registry. https://www.atsdr.cdc.gov/toxprofiles/tp46.pdf. Assessed March 16, 2017.
- ATSDR. (2007). Toxicological profile for lead. Agency for Toxic Substances and Disease Registry. https://www.atsdr.cdc.gov/toxprofiles/tp13.pdf. Assessed March 16, 2017.
- ATSDR. (2012). Toxicological profile for cadmium. Agency for Toxic Substances and Disease Registry. https://www.atsdr.cdc.gov/toxprofiles/tp5.pdf. Assessed March 16, 2017.
- Bukalo, E., Trako, E., Mitrovic, M., Behlulovic, D., & Rahmani, S. (2013). Soil monitoring in the Federation BiH. In Proceedings of the 48th Croatian and 8th international symposium of agronomist (pp. 65–69).Google Scholar
- Chen, C. L., Liao, M., & Huang, C. Y. (2005). Effect of combined pollution by heavy metals on soil enzymatic activities in areas polluted by tailings from Pb-Zn-Ag mine. Journal of Environmental Science, 17(4), 637–640.Google Scholar
- Christova, J., Christov, D., & Kuikin, S. (2007). Background contents of some minor and trace elements in the rocks on Bulgarian territory. Geologica Balcanica, 36, 65–76.Google Scholar
- Cipurkovic, A., Selimbasic, V., Tanjic, I., Micevic, S., Pelemis, D., & Celikovic, R. (2011). Heavy metals in sedimentary dust in industrial city of Lukavac. European Journal of Scientific Research, 54(3), 347–362.Google Scholar
- Cipurkovic, A., Trumic, I., Selimbasic, V., Djozic, A., Tunjic, J., & DjulovicJusic, Z. (2014a). Assessment of mercury pollution in soils along Jala and Spreca river banks in Bosnia and Herzegovina. Journal of Trends in the Development of Machinery and Associated Technology, 18, 183–186.Google Scholar
- Cipurkovic, A., Tunjic, J., Selimbasic, V., Djozic, A., & Trumic, I. (2014b). Assessment of heavy metal distribution and contamination in soils at Jala River Banks. European Journal of Scientific Research, 127(4), 392–405.Google Scholar
- Djozic, A. (2015). The presence of heavy metals in the soil and locally produced food in settlements around landfills with slag Divkovici/Plane from Thermal Power Plant Tuzla. Tuzla: Center for Ecology and Energy, Off-set.Google Scholar
- Djozic, A., Selimbasic, V., Cipurkovic, A., Crnkic, A., Hodzic, Z., & Trumic, I. (2014). Heavy metals in dust deposition in the vicinity of coal ash disposal site Divkovici II. Journal of Life Sciences, 8(5), 461–472.Google Scholar
- Eja, C. E., Ogri, O. R., & Arikpo, G. E. (2003). Bioconcentration of heavy metals in surface sediments from the Great Kwa river estuary, Calabar, Southeast Nigeria. Nigerian Journal of Environmental Sociology, 2, 247–256.Google Scholar
- Fernandez-Luqueno, F., Lopez-Valdez, F., Gamero-Melo, P., Suarez, S. L., Aguilera-Gonzalez, E. N., Martinez, A. I., et al. (2013). Heavy metal pollution in drinking water—A global risk for human health: A review. African Journal of Environmental Science and Technology, 7(7), 567–584.Google Scholar
- IARC. (1993a). IARC cancer databases. World Health Organization. http://www.iarc.fr. Accessed October 14, 2016.
- IARC. (1993b). IARC Monographs on the Evaluation of Carcinogenic Risk to Humans. Volume 58. Beryllium, cadmium, mercury and exposures in the glass manufacturing industry. World Health Organization. http://www.iarc.fr. Accessed April 13, 2017.
- IARC. (2006). IARC Monographs on the Evaluation of Carcinogenic Risk to Humans. Volume 87. Inorganic and Organic Lead Compounds. World Health Organization. http://www.iarc.fr. Accessed April 13, 2017.
- IARC. (2012). Arsenic, metals, fibers, and dust. Volume 100 C. A review of human carcinogens. World Health Organization. http://www.iarc.fr. Accessed April 13, 2017.
- IARC. (2017). IARC monographs on the evaluation of carcinogenic risk to humans. Agents classified by the IARC Monograps (Vols. 1–120). World Health Organization. http://monographs.iarc.fr/ENG/Classification/. Accessed Dec 4, 2017.
- Iqbal, H., Taseer, R., Anwar, S., Qadir, A., & Shahid, N. (2016). Human health risk assessment: Heavy metal contamination of vegetables in Bahawalpur, Pakistan. Bulletin of Environmental Studies, 1(1), 10–17.Google Scholar
- Ishaque, A. B., Johnson, L., Gerald, T., Boucaud, D., Okoh, J., & Tchounwou, P. B. (2006). Assessment of individual and combined toxicities of four non-essential metals (As, Cd, Hg and Pb) in the microtox assay. International Journal of Environmental Research and Public Health, 3(1), 118–120.Google Scholar
- ISO 10381-5. (2005). Soil quality-sampling. Part 5: Guidance on the procedure for the investigation of urban and industrial sites with regard to soil contamination. Geneva: International Organization for Standardization.Google Scholar
- ISO 10381-6. (2009). Soil quality—Sampling. Part 6: Guidance on the collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory. Geneva: International Organization for Standardization.Google Scholar
- ISO 11464. (2006). Soil quality—Pretreatment of samples for physico-chemical analysis. Geneva: International Organization for Standardization.Google Scholar
- Kabata-Pendias, A., & Pendias, H. (2001). Trace elements in soils and plants. Florida: CRC Press LLC.Google Scholar
- Katnoria, J. K., Arora, S., Bhardwaj, R., & Nagpal, A. (2011). Evaluation of genotoxic potential of industrial waste contaminated soil extracts of Amritsar, India. Journal of Environmental Biology, 32, 363–367.Google Scholar
- Kede, M. L. F. M., Correia, F. V., Conceicao, P. F., Salles Junior, S. F., Marques, M., Moreira, J. C., et al. (2014). Evaluation of mobility, bioavailability and toxicity of Pb and Cd in contaminated soil using TCLP, BCR and earthworms. International Journal of Environmental Research and Public Health, 11(11), 11528–11540.CrossRefGoogle Scholar
- Lah, B., Avbersek, M., Gorjanc, G., & MarinsekLogar, R. (2005). Toxic and genotoxic potential evaluation of soil samples by bioassays. Actaagriculturae Slovenica, 86(1), 27–38.Google Scholar
- Law of Agricultural Land. (2009). Official Gazette F BiH 52/09. http://www.fbihvlada.gov.ba/bosanski/zakoni/2009/zakoni/32bos.htm. Accessed March 13, 2017.
- Likuku, A. S., Mmolawa, K. B., & Gaboutloeloe, G. K. (2013). Assessment of heavy metal enrichment and degree of contamination around the copper-nickel mine in the Selebi Phikwe Region, Eastern Botswana. Environment and Ecology Research, 1(2), 32–40.Google Scholar
- Mmolawa, K. B., Likuku, A. S., & Gaboutloeloe, G. K. (2011). Assessment of heavy metal pollution in soils along major roadside areas in Botswana. African Journal of Environmental Science and Technology, 5(3), 186–196.Google Scholar
- Moucher, F., Gauthier, L., Mailhes, C., Jourdain, M. J., Ferrier, V., Triffault, G., et al. (2006). Biomonitoring of the genotoxic potential of aqueous extracts of soils and bottom ash resulting from municipal solid waste incineration using the comet and micronucleus tests on amphibian (Xenopuslaevis) larvae and bacterial assays (Mutatox and Ames tests). Science of the Total Environment, 335, 232–246.CrossRefGoogle Scholar
- Muller, G. (1969). Index of geo-accumulation in sediments of the Rhine River. Geo Journal, 2(3), 108–118.Google Scholar
- Olivieri, G., Brack, Ch., Müller-Spahn, F., Stähelin, H. B., Herrmann, M., & Renard, P. (2000). Mercury induces cell cytotoxicity and oxidative stress and increases β-amyloid secretion and tau phosphorylation in SHSY5Y neuroblastoma cells. Journal of Neurochemistry, 74(1), 231–236.CrossRefGoogle Scholar
- Ordinance on Identification of the Allowable Amounts of Harmful and Hazardous Substances in Soil and Methods of their Examination. (2009). Official Gazette F BiH 72/09. http://www.uip-zzh.com/files/zakoni/poljoprivreda/72-09.pdf. Accessed February 24, 2017.
- Osmanovic, S., Huseinovic, S., & Goletic, S. (2015). Assessment of heavy metals in vegetables grown in the Tuzla area. In Proceedings of the 9th scientific conference with international participation Quality 2015 (pp. 469–473).Google Scholar
- Pasalic, A., Bikic, F., & Goletic, S. (2015). The influence of soil pH and the addition of chelating agents on the lead content in certain plants. In Proceedings of the 9th scientific conference with international participation Quality 2015 (pp. 493–497).Google Scholar
- Product Information Sheet. (2010). PrestoBlue Viability Reagent Protocol. https://tools.thermofisher.com/content/sfs/manuals/PrestoBlue_Reagent_PIS_15Oct10.Pdf. Accessed September 29, 2016.
- Robinson, J. P., Sturgis, J., & Kumar, L. G. (2009). Immunohistochemical staining methods (5th ed.). California: Dako.Google Scholar
- Tunjic, J., Cipurkovic, A., & Selimbasic, V. (2015). Evaluation of possible applications of soil for agricultural purposes in terms of heavy metals and proximity to industrial facilities. In Proceedings of the third international scientific-expert symposium agricultural production and environmental protection in the development of rural areas (pp. 224–237).Google Scholar
- U.S. EPA. (2000a). Cadmium compounds. U.S Environmental Protection Agency. https://www.epa.gov/sites/production/files/2016-09/documents/cadmium-compounds.pdf. Assessed March 16, 2017.
- U.S. EPA. (2011). Lead compounds. U.S Environmental Protection Agency. https://www.epa.gov/sites/production/files/2016-09/documents/lead-compounds.pdf. Assessed March 16, 2017.
- U.S. EPA. (2000b). Mercury compounds. U.S Environmental Protection Agency. https://www.epa.gov/sites/production/files/2016-09/documents/mercury-compounds.pdf. Assessed March 16, 2017.
- WHO. (2000). Environmental Health Criteria 214. Human Exposure Assessment. World Health Organization. http://www.chec.pitt.edu/documents/un-environ-prog.pdf. Accessed February 23, 2017.