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Environmental Geochemistry and Health

, Volume 39, Issue 6, pp 1595–1605 | Cite as

Abundance and distribution of antibiotic resistance genes in a full-scale anaerobic–aerobic system alternately treating ribostamycin, spiramycin and paromomycin production wastewater

  • Mei Tang
  • Xiaomin DouEmail author
  • Chunyan Wang
  • Zhe Tian
  • Min Yang
  • Yu ZhangEmail author
Original Paper

Abstract

The occurrence of antibiotic-resistant bacteria and antibiotic resistance genes (ARGs) has been intensively investigated for wastewater treatment systems treating single class of antibiotic in recent years. However, the impacts of alternately occurring antibiotics in antibiotic production wastewater on the behavior of ARGs in biological treatment systems were not well understood yet. Herein, techniques including high-capacity quantitative PCR and quantitative PCR (qPCR) were used to investigate the behavior of ARGs in an anaerobic–aerobic full-scale system. The system alternately treated three kinds of antibiotic production wastewater including ribostamycin, spiramycin and paromomycin, which referred to stages 1, 2 and 3. The aminoglycoside ARGs (52.1–79.3%) determined using high-capacity quantitative PCR were the most abundant species in all sludge samples of the three stages. The total relative abundances of macrolide–lincosamide–streptogramin (MLS) resistance genes and aminoglycoside resistance genes measured using qPCR were significantly higher (P < 0.05) in aerobic sludge than in sewage sludge. However, the comparison of ARGs acquired from three alternate stages revealed that MLS genes and the aminoglycoside ARGs did not vary significantly (P > 0.05) in both aerobic and anaerobic sludge samples. In aerobic sludge, one acetyltransferase gene (aacA4) and the other three nucleotidyltransferase genes (aadB, aadA and aadE) exhibited positive correlations with intI1 (r 2 = 0.83–0.94; P < 0.05), implying the significance of horizontal transfer in their proliferation. These results and facts will be helpful to understand the abundance and distribution of ARGs from antibiotic production wastewater treatment systems.

Keywords

Antibiotic production wastewater Antibiotic resistance genes Aminoglycoside resistance genes Wastewater treatment systems Alternate treatments 

Notes

Acknowledgements

This project is supported by National Natural Scientific Foundation of China (No. 21437005) and special fund of State Key Joint Laboratory of Environmental Simulation and Pollution Control (15L03ESPC).

Supplementary material

10653_2017_9987_MOESM1_ESM.docx (126 kb)
Supplementary material 1 (DOCX 126 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  1. 1.State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijingChina
  2. 2.College of Environmental Science and EngineeringBeijing Forestry UniversityBeijingChina
  3. 3.Department of Biology and Chemical EngineeringNanyang Institute of TechnologyNanyangChina
  4. 4.University of Chinese Academy of SciencesBeijingChina

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