Environmental Geochemistry and Health

, Volume 34, Issue 3, pp 365–374 | Cite as

Effect of insecticides alone and in combination with fungicides on nitrification and phosphatase activity in two groundnut (Arachis hypogeae L.) soils

  • M. Srinivasulu
  • G. Jaffer Mohiddin
  • K. Subramanyam
  • V. Rangaswamy
Original Paper


The effect of selected pesticides, monocrotophos, chlorpyrifos alone and in combination with mancozeb and carbendazim, respectively, was tested on nitrification and phosphatase activity in two groundnut (Arachis hypogeae L.) soils. The oxidation of ammonical nitrogen was significantly enhanced under the impact of selected pesticides alone and in combinations at 2.5 kg ha−1 in black soil, and furthermore, increase in concentration of pesticides decreased the rate of nitrification, whereas in the case of red soil, the nitrification was increased up to 5.0 kg ha−1 after 4 weeks, and then decline phase was started gradually from 6 to 8 weeks of incubation. The activity of phosphatase was increased in soils, which received the monocrotophos alone and in combination with mancozeb up to 2.5 and 5.0 kg ha−1, whereas the application of chlorpyrifos singly and in combination with carbendazim at 2.5 kg ha−1 profoundly increased the phosphatase activity after 20 days of incubation, in both soils. But higher concentrations of pesticides were either innocuous or inhibitory to the phosphatase activity.


Pesticides Combinations Nitrification Phosphatase activity Groundnut (Arachis hypogeae L.) soils 



We are grateful to the University Grants commission (UGC), New Delhi, India, for financial assistance.


  1. Alexander, M. (1977). Introduction to soil microbiology (2nd ed., pp. 113–330). New York: Wiley.Google Scholar
  2. Barnes, H., & Folkard, B. R. (1951). The determination of nitrite. Analyst, 76, 599–603.CrossRefGoogle Scholar
  3. Caceres, T., Wenxiang, H., Megharaj, M., & Naidu, R. (2009). Effect of insecticide fenomiphos on soil microbial activities in Australian and Ecuadorean soils. Journal of Environmental Science and Health Part B, 44(1), 13–17.CrossRefGoogle Scholar
  4. Chu, X. F., Hua, P., Xuedong, W., Xiao, S., Min, F. Bo., & Yunlong, Y. (2008). Degradation of chlorpyrifos alone and in combination with chlorothalonil and their effects on soil microbial populations. Journal of Environmental Sciences, 20, 464–469.CrossRefGoogle Scholar
  5. Cycon, M., Piotrowska-Seget, Z., Kaczynska, A., & Kozdroj, J. (2006). Microbiological characteristics of a loamy sand soil exposed to tebuconazole and λ-cyhalothrin under laboratory conditions. Ecotoxicology, 15(8), 639–646.CrossRefGoogle Scholar
  6. Cycon, M., Piotrowska-Seget, Z., & Kozdroj, J. (2010). Responses of indigenous microorganisms to a fungicidal mixture of mancozeb and dimethomorph added to sandy soils. International Biodeterioration & Biodegradation., 64, 316–323.CrossRefGoogle Scholar
  7. Das, A. C., & Mukherjee, D. (1994). Effect of insecticides on the availability of nutrients, nitrogen fixation, and phosphate solubility in the rhizosphere soil of rice. Biology and Fertility of Soils, 18, 37–41.CrossRefGoogle Scholar
  8. Fenchel, T., King, G. M., & Blackburn, T. H. (1998). Bacterial biogeochemistry : The ecophysiology of Mineral Cycling, 2nd edition, Academic Press, pp. 201–204.Google Scholar
  9. Getenga, Z. M., Jondiko, J. I. O., Wandiga, S. O., & Beck, E. (2000). Dissipation behavior malthion and dimethoate residues from the soil and their uptake by garden pea (Pisum sativum). Bulletin of Environmental Contamination and Toxicology, 64(3), 359–367.CrossRefGoogle Scholar
  10. Getenga, N. C., & Weil, R. R. (2006). Elements of the nature and properties of soils (p. 5). Prentice Hall: Englewood Cliffs.Google Scholar
  11. Giraddi, R. S., Lingappa, S., & Hegde, R. (1999). Bioefficacy of new wettable powders on leaf eating caterpillars of groundnut. Pestol, 23(7), 57–59.Google Scholar
  12. Graebing, P., Frank, M., & Chib, J. S. (2002). Effects of fertilizer and soil components on pesticide photolysis. Journal of Agricultural Food and Chemistry, 50, 7332–7339.CrossRefGoogle Scholar
  13. Guha, P., & Chandrasekhar, S. C. (2001). Efficacy and residues studies of propiconazole 25% EC in groundnut. Pestalogy, 23(7), 57–59.Google Scholar
  14. Gundi, V. A. K. B., Narasimha, G., & Reddy, B. R. (2005). Interaction effects of soil insecticides on microbial populations and dehydrogenase activity in a black clay soil. Journal of Environmental Science and Health, 40, 269–281.Google Scholar
  15. Gundi Vijay, A. K. B., Naraharikumar, V., & Reddy, B. R. (2006). Effect of insecticides on nitrogen mineralization and nitrifying organisms in a black vertisol soil. Indian Journal of Microbiology, 46(2), 129–134.Google Scholar
  16. Hansson, G. B., Klemedtsson, L., Stenstorm, J., & Torstensson, L. (1991). Testing the influence of chemicals on soil autotrophic ammonium oxidation. Environmental Toxicology and Water Quality, 6, 351–360.CrossRefGoogle Scholar
  17. Jackson, M. L. (1971). Soil chemical analysis. New Delhi: Prentice Hall India.Google Scholar
  18. Jaya Madhuri, R., & Rangaswamy, V. (2009). Biodegradation of selected insecticides by Bacillus and Pseudomonas sps. in groundnut fields. Toxicology International, 16(2), 127–132.Google Scholar
  19. Kinney, C. A., Mandernack, K. W., & Mosier, A. R. (2005). Laboratory investigations into the effects of the pesticides mancozeb, chlorothalonil, and prosulfuron on nitrousoxide and nitric oxide production in fertilized soil. Soil Biology and Biochemistry, 37, 837–850.CrossRefGoogle Scholar
  20. Lodhi, A. M., Malik, N., & Azam, F. (1994). Effect of baythroid on nitrogen transformations in soil. Biology and Fertility of Soils, 197, 173–176.CrossRefGoogle Scholar
  21. Man, L., & Zucong, C. (2009). Effects of chlorothalonil and carbendazim on nitrification and denitrification in soils. Journal of Environmental Sciences, 21, 458–467.CrossRefGoogle Scholar
  22. Mazellier, P., Leroy, E., Laat, J. T., & Legube, B. (2003). Degradation of carbendazim by UV/H2O2 investigated bykinetic modeling. Environmental Chemistry Letters, 1, 68–72.CrossRefGoogle Scholar
  23. Megharaj, M., Kookana, K., & Singleton, S. (1999). Activities of fenamiphos on native algae population and some enzyme activities in soil. Soil Biology and Biochemistry, 39, 1549–1553.CrossRefGoogle Scholar
  24. Monkiedje, A., Llori, M. O., & Spiteller, M. (2002). Soil quality changes resulting from the application of the fungicides mefenoxam and metalaxyl to a sandy loam soil. Soil Biology and Biochemistry, 34, 1939–1948.CrossRefGoogle Scholar
  25. Nazima, R., & Zafar, R. A. (2010). Effect of the fungicide mancozeb at different application rates on enzyme activities in a silt loam soil of the Kashmir Himalaya, India. Tropical Ecology, 51(2), 199–205.Google Scholar
  26. Pell, M., Stenberg, B., & Torstensson, L. (1998). Potential denitrifacation and nitrification tests for evaluation of pesticide effects in soil. Ambio, 27(1), 24–28.Google Scholar
  27. Piotrowska, S. Z., Engel, R., Nowak, E., & Kozdroj, J. (2008). Sucessive soil treatment with captan or oxytetracycline affects non-target microorganisms. World Journal of Microbiology & Biotechnology, 24, 2843–2848.CrossRefGoogle Scholar
  28. Posen, S. (1967). Alkaline phosphatase. Annals of Internal Medicine, 67, 183–203.Google Scholar
  29. Rahmansyah, M., Antonius, S., & Sulistinah, N. (2009). Phosphatase and urease instability caused by pesticides present in soil improved by grounded rice straw. ARPN Journal of Agricultural and Biological Science, 4(2), 56–62.Google Scholar
  30. Rangaswamy, V., & Venkateswarlu, K. (1990). Stimulation of ammonification and nitrification in soils by the insecticides, monocrotophos and quinalphos. Biomedical and Environmental Sciences, 3, 391–396.Google Scholar
  31. Rangaswamy, V., & Venkateswarlu, K. (1993). Ammonification and nitrification in soils, and nitrogen fixation by Azospirillum sp. as influenced by cypermethrin and fenvalerate. Agriculture, Ecosystems & Environment, 45, 311–317.CrossRefGoogle Scholar
  32. Rangaswamy, V., & Venkateswarlu, K. (1996). Phosphatase activity in groundnut soils as influenced by selected insecticides. Journal of Environmental Biology, 17, 115–119.Google Scholar
  33. Ranney, T. A., & Bartlett, R. J. (1972). Rapid field determination of nitrate in natural waters. Communications in Soil Science and Plant Analysis, 3, 183–186.CrossRefGoogle Scholar
  34. Saison, C., Natasha, J. W., Anu, K., & Rai, S. K. (2009). Effect of thiobencarb in combination with molinate and chlorpyrifos on selected soil microbial processes. Journal of Environmental Science and Health Part B, 44, 226–234.CrossRefGoogle Scholar
  35. Schneider, K., Turrion, M.-B., Grierson, B. F., & Gallardo, J. F. (2001). Phosphatase activity, microbial phosphorus, and fine root growth in forest soil in the Sierra de Gata, western central Spain. Biology and Fertility Soils, 34, 151–155.CrossRefGoogle Scholar
  36. Sha, J. J. (1999). A manual of industrial and chemical production: agricultural chemicals (pp. 13–14). Beijing: Chemical Industry Press.Google Scholar
  37. Shin, C. L., Funke, B. R., & Schulz, J. T. (1972). Effects of some organophosphate and carbamate insecticides on nitrification and legume growth. Plant and Soil, 37, 489–496.CrossRefGoogle Scholar
  38. Shukla, A. K., & Mishra, R. R. (1997). Influence of herbicides on microbial population and enzyme activities in potato (Solanum tuberosum) field soil. Indian Journal of Agricultural Science, 67, 610–611.Google Scholar
  39. Sikora, L. J., Kaufman, D. D., & Horng, L. C. (1990). Enzyme activity in soils showing enhanced degradation of organophosphate insecticides. Biology and Fertility of Soils, 9, 14–18.CrossRefGoogle Scholar
  40. Sousa, P. J., Rodrigues, J. M., Loureiro, L. S., Soares, A. M. V. M., Jones, S. E., & Forster, B. (2004). Ring–Testing and field–validation of a terrestrial model ecosystem (TME)–an instrument for testing potentially harmful substances: Effects of carbendazim on soil microbial parameters. Ecotoxicology, 13, 43–60.CrossRefGoogle Scholar
  41. Tabatabai, M.A. (1994). Soil enzymes. In: Mickelson, J.M. Bigham (Eds.).Methods of soil analysis. Part 2. Microbial and Biochemical properties, Soil Science Society of America Journanl. Madison, Wisconsin, pp. 775–826.Google Scholar
  42. Tu, C. M. (1990). Effect of four experimental insecticides on enzyme activities and levels of adenosine in mineral and organic soils. Journal of Environmental Science and Health Part B, 25, 787–800.CrossRefGoogle Scholar
  43. Tu, C. M., Marks, C. F., & Elliot, J. M. (1996). Effects of nematicides on pratylenchus penetrans, soil nitrification and growth of flue cured tobacco. Bulletin of Environmental Contamination and Toxicology, 57, 924–931.CrossRefGoogle Scholar
  44. Vonk, J. W. (1991). Testing of pesticides for side effects on nitrogen for side-effects on nitrogen conversions in soil. Toxicology and Environmental Chemistry, 30, 241–248.CrossRefGoogle Scholar
  45. Xie, X. M., Liao, M., Huanq, C. Y., Liu, W. P., & Abid, S. (2004). Effects of pesticides on soil biochemical characteristics of paddy soil. Journal of Environmental Sciences, 16(2), 252–255.Google Scholar
  46. Yan, H., Danden, W., Dong, B., Feifan, T., Baichuan, W., Hua, F., et al. (2011). Dissipation of carbendazim and chloramphenicol alone and combination and heir effects on soil fungal:bacterial ratios and soil enzyme activities. Chemosphere,. doi: 10.1016/j.Chemosphere.2011.03.038.Google Scholar
  47. Yun Long, Y. U., Min, S., Hua, F., Xiao, W., & Xiao, Q. C. (2006). Responses of soil microorganisms and enzymes to repeated application of chlorothalonil. Journal of Agricultural and Food Chemistry, 54, 10070–10075.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • M. Srinivasulu
    • 1
  • G. Jaffer Mohiddin
    • 1
  • K. Subramanyam
    • 2
  • V. Rangaswamy
    • 1
  1. 1.Department of MicrobiologySri Krishnadevaraya UniversityAnantapurIndia
  2. 2.Plant Molecular Biology Laboratory, Department of BiotechnologyBharathidasan UniversityTiruchirappalliIndia

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