Principles and application of an in vivo swine assay for the determination of arsenic bioavailability in contaminated matrices
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The assessment of arsenic (As) bioavailability from contaminated matrices is a crucial parameter for reducing the uncertainty when estimating exposure for human health risk assessment. In vivo assessment of As utilising swine is considered an appropriate model for human health risk assessment applications as swine are remarkably similar to humans in terms of physiology and As metabolism. While limited in vivo As bioavailability data is available in the literature, few details have been provided regarding technical considerations for performing in vivo assays. This paper describes, with examples, surgical, experimental design and analytical issues associated with performing chronic and acute in vivo swine assays to determine As bioavailability in contaminated soil and food.
KeywordsArsenic Bioavailability In vivo Swine
This research was funded through the Australian Research Council Linkage Grant Scheme, Grant number LP0347301. In vivo assays were approved and conducted according to application No. 17/02 of the Institute for Medical and Veterinary Science Animal Ethics Committee. The authors would like to acknowledge the support of the Centre for Environmental Risk Assessment and Remediation (University of South Australia), Centre for Pharmaceutical Studies (University of South Australia), and the Institute for Medical and Veterinary Science for this research.
- Agilent Technologies. (2006). Determination of heavy metals in whole blood by ICP-MS. Agilent Technologies publication number 5988-0533EN.Google Scholar
- Casteel, S. W., Brown, L. D., Dunsmore, M. E., Weis, C. P., Henningsen, G. M., Hoffman, E., Brattin, W., Hammon, T. L. (1997). Relative bioavailability of arsenic in mining wastes. Document control no. 4500-88-AORH. U.S. Environmental Protection Agency, Region 8, Denver, CO.Google Scholar
- Freeman, G. B., Schoof, R. A., Ruby, M., Davis, A. O., Dill, S. C., Liao, S. C., et al. (1995). Bioavailability of arsenic in soil and house dust impacted by smelter activities following oral administration in cynomolgus monkeys. Fundamental and Applied Toxicology, 28, 215–222.CrossRefGoogle Scholar
- Holliman, C. J., Kenfield, K., Nutter, E., Saffle, J. R., & Warden, G. D. (1982). Technique for acute subpubic catheterisation of urinary bladder in the pig. American Journal of Veterinary Research, 43, 1056–1057.Google Scholar
- Juhasz, A. L., Smith, E., Weber, J., Rees, M., Rofe, A., Kuchel, T., et al. (2006). In vivo assessment of arsenic bioavailability in rice and its significance for human health risk assessment. Environmental Health Perspectives, 114, 1826–1831.Google Scholar
- Juhasz, A. L., Smith, E., Weber, J., Rees, M., Rofe, A., Kuchel, T., et al. (2007). Comparison of in vivo and in vitro methodologies for the assessment of arsenic bioavailability in contaminated soils. Chemosphere. doi: 10.1016/j.chemosphere.2007.05.018.
- Kelly, M. E., Brauning, S. E., Schoof, R. A., & Ruby, M. V. (2002). Assessing oral bioavailability of metals in soil. Ohio: Battelle Press.Google Scholar
- Lien, H. C., Tsai, T. F., Lee, Y. Y., & Hsiao, C. H. (2001). Merkel cell carcinoma and chronic arsenicism. Journal of the American Academy of Dermatology, 41, 641–643.Google Scholar
- Thurmon, J. C., Nelson, D. R., Bevill, R. F., Harrnigton, G. W., & Magee, D. N. (1987). Surgical procedure for chronic bilary sample collection in pigs. American Journal of Veterinary Research, 48, 988–989.Google Scholar
- USEPA. (1998). Method 3051A, microwave assisted acid digest of sediments, sludges, soils and oils. In USEPA methods, pp 3051A/1-24.Google Scholar
- Weis, C. P., & LaVelle, J. M. (1991). Characteristics to consider when choosing an animal model for the study if lead bioavailability. Chemical Speciation and Bioavailability, 3, 113–119.Google Scholar