Possible linkages between lignite aquifers, pathogenic microbes, and renal pelvic cancer in northwestern Louisiana, USA
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In May and September, 2002, 14 private residential drinking water wells, one dewatering well at a lignite mine, eight surface water sites, and lignite from an active coal mine were sampled in five Parishes of northwestern Louisiana, USA. Using a geographic information system (GIS), wells were selected that were likely to draw water that had been in contact with lignite; control wells were located in areas devoid of lignite deposits. Well water samples were analyzed for pH, conductivity, organic compounds, and nutrient and anion concentrations. All samples were further tested for presence of fungi (cultures maintained for up to 28 days and colonies counted and identified microscopically) and for metal and trace element concentration by inductively-coupled plasma mass spectrometry and atomic emission spectrometry. Surface water samples were tested for dissolved oxygen and presence of pathogenic leptospiral bacteria. The Spearman correlation method was used to assess the association between the endpoints for these field/laboratory analyses and incidence of cancer of the renal pelvis (RPC) based on data obtained from the Louisiana Tumor Registry for the five Parishes included in the study. Significant associations were revealed between the cancer rate and the presence in drinking water of organic compounds, the fungi Zygomycetes, the nutrients PO4 and NH3, and 13 chemical elements. Presence of human pathogenic leptospires was detected in four out of eight (50%) of the surface water sites sampled. The present study of a stable rural population examined possible linkages between aquifers containing chemically reactive lignite deposits, hydrologic conditions favorable to the␣leaching and transport of toxic organic compounds from the lignite into the groundwater, possible microbial contamination, and RPC risk.
KeywordsLeptospires Lignite aquifers Medical geology Natural water contamination Renal pelvic cancer
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We thank Peter Warwick, Alexander Karlsen, Chacko John, Douglas Carlson, John Lovelace, Jon Kolak, Donna Francy, and Jerry McLarty for their scientific insights, critical reviews, and kind assistance. The Dolet Hills Mining Co. generously provided access for us to collect samples. Marie Burleson, Ohio Department of Health, assisted in identifying fungal species, and Paul Levett, Centers for Disease Control and Prevention in Atlanta, kindly provided leptospiral culture material and DNA. The work was supported in part by the USGS Energy Resources Program (Brenda Pierce, Program Coordinator) and by the USGS Eastern Energy Resources Team (Ione Taylor, Chief Scientist). Other support was provided by the USGS Water Resources Discipline and the Eastern Region (Steve Hindall, Tom Armstrong, Dan Hippe, Cathy Hill). Many thanks to Steve Suitt for GIS assistance.
- Abouzied, M. M., Horvath, A. D., Podlesny, P. M., Regina, N. P., Metodiev, V. D., Kamenova-Tozeva, R. M., Niagolova, N. D., Stein, A. D., Petropoulos, E. A., & Ganev, V. S. (2002). Ochratoxin A concentrations in food and feed from a region with Balkan endemic nephropathy. Food Additives and Contaminants, 19, 755–764.CrossRefGoogle Scholar
- Armstrong, B., Garrod, A., & Doll, R. (1976). A retrospective strudy of renal cancer with special reference to coffee and animal consumption. British Journal of Cancer, 33, 127–136.Google Scholar
- Baruthio, F. (1992). Toxic effects of chromium and its compounds. Biological Trace Element Research, 32, 145–153.Google Scholar
- Blot, W. J., & Fraumeni, J. F. Jr., (1979). Geographic patterns of renal cancer in the United States. Journal of the National Cancer Institute, 63, 363–366.Google Scholar
- Boorman, G. A. (1989). Toxicology and carcinogenesis studies of ochratoxin A (cas no. 303–47-9) in F344/N rats (gavage studies). NIH Publication No. 89-2813, National Toxicology Program, U.S. Department of Health and Human Services, National Institutes of Health, Bethesda.Google Scholar
- Bunnell, J. E., Bushon, R. N., Stoeckel, D. M., Gifford, A. M., Beck, M., Lerch, H. E., Shi, R., McGee, B., Hanson, B. C., Kolak, J., & Warwick, P. D. (2003). U.S. Geological Survey Open-File Report 03-374.␣Preliminary geochemical, microbiological, and epidemiological investigations into possible linkages between lignite aquifers, pathogenic microbes, and kidney disease in northwestern Louisiana (http://pubs.usgs.gov/of/2003/of03-374) (31 March 2004).
- Chawner, W. D. (1936). Geology of Catahoula and Concordia Parishes. Geological Bulletin Number 9, Department of Conservation. Louisiana Geological Survey, Baton Rouge, LA.Google Scholar
- Chernozemsky, I. N., Stoyanov, I. S., Petkova-Bocharova, T. K., Nicolov, I. G., Draganov, I. V., Stoichev, I. I., Tancev, Y., Naidenov, D., & Kalcheva, N. D. (1977). Geographic correlation between the occurrence of endemic nephropathy and urinary tract tumours in Vratza District, Bulgaria. International Journal of Cancer, 19, 1–11.Google Scholar
- Fillastre, J. P. (1997). Ochratoxin-induced animal and human nephrotoxicity. Bulletin de l’Académie Nationale de Médecine, 181, 1447–1460.Google Scholar
- Fored, C. M., Ejerblad, E., Lindblad, P., Fryzed, J. P., Dickman, P. W., Signorello, L. B., Lipworth, L., Elinder, C. -G., Blot, W. J., McLaughlin, J. K., Zack, M. M., & Nyren, O. (2001). Acetaminophen, aspirin, and chronic renal failure. New England Journal of Medicine, 345, 1801–1808.CrossRefGoogle Scholar
- Gallieni, M., Brancaccio, D., Cozzolino, M., & Sabbioni, E. (1996). Trace elements in renal failure: Are they clinically important? Nephrology Dialysis Transplantation, 11, 1232–1235.Google Scholar
- Glenk, R. (1921). Louisiana lignite. Bulletin 8, Louisiana Department of Conservation, LA.Google Scholar
- Gravekamp, C., Van de Kemp, H., Franzen, M., Carrington, D., Schoone, G. J., Van Eys, G. J. J. M., Everard, C. O. R., Hartskeerl, R. A., & Terpstra, W. J. (1993). Detection of seven species of pathogenic leptospires by PCR using two sets of primers. Journal of General Microbiology, 139, 1691–1700.Google Scholar
- Larone D. H. (2002). Medically important fungi – A guide to identification (4th ed.), Washington, D.C.: American Society for Microbiology PressGoogle Scholar
- Lindeman, R. D. (1989). Trace minerals and the kidney: An overview. Journal of the American College of Nutrition, 8, 285–291.Google Scholar
- Makarov, V., Topakbashyan, S., & Dinev, I. (1967). The content of trace elements in biological materials from people who have died of endemic nephropathy. Dokladi Na Bolgarskata Akademiya Na Naukite, 20, 633–636.Google Scholar
- Maksimovic, Z. J., & Djujic, I. (1997). Selenium deficiency in Serbia and possible effects on health. Biomedical and Environmental Sciences, 10, 300–306.Google Scholar
- McLaughlin, J. K., Blot W. J., Devesa S. S. & Fraumeni, J. F. Jr. (1996). Renal cancer. In: D. Scheottenfeld Jr. & J. F. Fraumeni (Eds.), Cancer epidemiology and prevention (2nd ed., pp. 1142–1155). New York: Oxford University Press.Google Scholar
- Meagher, D. P., & Aycock, L. C. (1942). Louisiana lignite. Geological Pamphlet Number 3, Department of Conservation. Louisiana Geological Survey, Baton Rouge, LA.Google Scholar
- Minciu, R., Botoca, M. R., Cumpanas, A., & Hodor, M. (2003). Recent data on the epidemiology of urothelial tumors in our region. Timisoara Medical Journal, 53(1), 44–48.Google Scholar
- Nikolov, I. G., Petkova-Bocharova, D., Castegnaro, M., Pfohl-Leskowicz, A., Gill, C., Day, N., & Chernozemsky, I. N. (1996). Molecular and epidemiological approaches to the etiology of urinary tract tumors in an area with Balkan endemic nephropathy. Journal of Environmental Pathology, Toxicology and Oncology: Official Organ of the International Society for Environmental Toxicology and Cancer, 15, 201–207.Google Scholar
- Orem, W. H. & Tatu, C. A. (2001). USGS Fact Sheet. Health Effects of Toxic Organic Compounds from Coal – The case of Balkan endemic nephropathy (BEN). (http://pubs.usgs.gov/fs/fs004-01) (31 March 2004).
- Orem, W. H., Tatu, C. A., Feder, G. L., Finkelman, R. B., Lerch, H. E., Maharaj, S., Szilagyi, D., Dumitrascu, V., Paunescu, V., & Margineanu, F. (2002). Environment, geochemistry, and the etiology of Balkan Endemic Nephropathy: Lessons from Romania. Facta Universitatis, Medicine and Biology Series, 9, 39–46.Google Scholar
- Orem, W. H., Tatu, C. A., Lerch, H. E., Maharaj, S., Pavlovic, N., Paunescu, V., & Dumitrascu, V. (2004). Identification and environmental significance of the organic compounds in water supplies associated with a Balkan endemic nephropathy region in Romania. Journal of Environmental Health Research, 3, 53–61.Google Scholar
- Petrinska-Venkovska, S. (1960). Morphological studies on endemic nephritis (in Bulgarian). In: A. Puchlev (Ed.), Endemic nephritis in Bulgaria (pp. 72–90). Medizina I Fiskultura, Sofia.Google Scholar
- Radonic, M., & Radosevic, Z. (1992). Clinical features of Balkan endemic nephropathy. Food and Chemical Toxicology: An International Journal Published for the British Industrial Biological Research Association, 30, 189–192.Google Scholar
- Ries, L. A. G., Eisner, M. P., Kosary, C. L., Hankey, B. F., Miller, B. A., Clegg, L. & Edwards, B. K. (Eds.) (2001). National Cancer Institute, Bethesda, MD. SEER Cancer Statistics Review, 1973–1998. (http://seer.cancer.gov/Publications/CSR1973_1998).
- Roland, H. L., Jenkins, G. M., & Pope, D. E. (1976). Lignite - Evaluation of near-surface deposits in northwest Louisiana. Mineral Resources Bulletin Number 2, Louisiana Geological Survey, Baton Rouge, LA.Google Scholar
- Sargent, B. P. (2002). Water use in Louisiana, 2002. Water Resources Special Report No. 15, U.S. Geological Survey, Baton Rouge, LA.Google Scholar
- Snider, J. L. (1982). Premining hydrology of the lignite area in southeastern DeSoto Parish, Louisiana. Water Resources Technical Report No. 29, Louisiana Department of Transportation and Development, Office of Public Works, Baton Rouge, LA.Google Scholar
- Stoev, S. D. (1998). The role of ochratoxin A as a possible cause of Balkan endemic nephropathy and its risk evaluation. Veterinary and Human Toxicology, 40, 352–360.Google Scholar
- Tanchev, Y., Evstatiev, Z., Dorossiev, D., Penchava, J., & Zvetkov, G. (1956) Studies on the nephritides in the District of Vratza (in Bulgarian). Savremena Medicina (Sofia), 7, 14–29.Google Scholar
- Tanchev, Y., Naidenov, D., Dimitrov, T., & Karlova, E. (1970). Neoplastic diseases and endemic nephritis (in Bulgarian). Vatreshni Bolesti, 9, 21–29.Google Scholar
- Tatu, C. A., Orem, W. H., Finkelman, R. B., & Feder, G. L. (1998). The etiology of Balkan endemic nephropathy: Still more questions than answers. Environmental Health Perspectives, 106, 689–700.Google Scholar
- Tatu, C. A., Orem, W. H., Feder, G. L., Finkelman, R. B., Szilagyi, D. N., Dumitrascu, V., Margineanu, F., & Paunescu, V. (2000). Additional support for the role of the Pliocene lignite derived organic compounds in the etiology of Balkan endemic nephropathy. Journal of Medicine and Biochemistry, 4, 95–101.Google Scholar
- Tully, J. (1996). Coal Fields of the Conterminous United States. U.S. Geological Survey Open-File Report OF 96–92.Google Scholar
- Vanholder, R., Cornelis, R., Dhondt, A., & Lameire, N. (2002). The role of trace elements in uraemic toxicity. Nephrology Dialysis Transplantation, 17 [Suppl 2], 2–8.Google Scholar
- Woo, T. H., Patel, B. K., Smythe, L. D., Symonds, M. L., Norris, M. A., & Dohnt, M. F. (1997). Identification of pathogenic Leptospira genospecies by continuous monitoring of fluorogenic hybridization probes during rapid-cycle PCR. Journal of Clinical Microbiology, 35, 3140–3146.Google Scholar