Effects of Bisphenol A on redox balance in red blood and sperm cells and spermatic quality in zebrafish Danio rerio

  • C. R. Silveira
  • A. S. Varela Junior
  • C. D. Corcini
  • S. L. Soares
  • A. N. Anciuti
  • M. T. Kütter
  • P. E. MartínezEmail author


Bisphenol-A (BPA) is a potential endocrine disruptor besides being associated with oxidative damage in several vertebrate classes. In the present study we investigated oxidative effects in erythrocytes and sperm cells as well as spermatic quality in Danio rerio exposed to 14 days at BPA concentrations of 2, 10 and 100 μg/L. Organelles structure, reactive species of oxygen (ROS) and lipoperoxidation (LPO) on erythrocytes and sperm cells were measured by flow cytometry and spermatic parameters were analyzed by the computer-assisted sperm analysis (CASA) system. For both cell types, when compared with control BPA treatment induced a significant increase in ROS and LPO production causing the membrane fluidity disorder, loss of membrane integrity and mitochondrial functionality. Furthermore, it was found a significant increase in DNA fragmentation in erythrocytes of zebrafish BPA exposed. Regarding the spermatic quality, results showed lower sperm motility in animals exposed to BPA, and alterations on velocity parameters of spermatozoa. Thus, the present study concludes that BPA affects the oxidative balance of both cell types, and that can directly affects the reproductive success of the adult Danio rerio. The sensitivity of erythrocytes to oxidative damage induced by BPA was similar to sperm cells, indicating a potential use of blood cells as indicators of oxidative damage present in fish sperm.


Bisphenol-A Blood cells Danio rerio Oxidative stress Reproduction Sperm quality 



We would like to acknowledge the Fundação de Amparo à pesquisa Rio Grande do Sul (FAPERGS). ASV Jr (Process number 307195/2015-7) and CDC (Process number 306356/2014-7) are a research fellow from the Brazilian Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted (Experimental Animal Ethic Committee CEUA – FURG Process number 23116.000355/2016-72).


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • C. R. Silveira
    • 1
  • A. S. Varela Junior
    • 1
  • C. D. Corcini
    • 2
  • S. L. Soares
    • 2
  • A. N. Anciuti
    • 2
  • M. T. Kütter
    • 1
  • P. E. Martínez
    • 1
    Email author
  1. 1.Reprodução Animal Comparada, Instituto de Ciências BiológicasUniversidade Federal do Rio GrandeRio GrandeBrazil
  2. 2.Reprodução Animal, Faculdade de VeterináriaUniversidade Federal de PelotasPelotasBrazil

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