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Chronic toxicity and biochemical response of Apis cerana cerana (Hymenoptera: Apidae) exposed to acetamiprid and propiconazole alone or combined

  • Wensu Han
  • Yemeng Yang
  • Jinglin GaoEmail author
  • Dongxiang ZhaoEmail author
  • Chengcai Ren
  • Shijie Wang
  • Shan Zhao
  • Yihai Zhong
Article

Abstract

Acetamiprid and ergosterol-inhibiting fungicide (EBI) are frequently applied to many flowering plants, while honey bees are pollinating agents or pollinators of the flowers. Hence honey bees are often exposed to these pesticides. But until now, the effects of theses combinations at field-realistic doses on honey bee health have been poorly investigated. In this study, we explore the synergistic mortality and some physiological effects in surviving honey bees after chronic oral exposure to acetamiprid and/or propiconazole in the laboratory. The results indicated that chronic combined exposure to acetamiprid and propiconazole produced a significant synergistic effect on mortality both for newly emerged bees (50% mortality in 7.2 days) and forager bees (50% mortality in 4.8 days). Honey bee weight of newly emerged bees was decreased after feeding food with a field concentration of acetamiprid and propiconazole, alone or combined for 10 days. Combination of acetamiprid and propiconazole also modulated the activities of P450s, GST and CAT in newly emerged bees and forager bees than either alone, but neither pesticide affected the activity of AChE. These results show that chronic combined exposure to pesticides of relatively low toxicity may caused severely physiological disruptions that could be potentially damaging for the honey bees.

Keywords

Apis cerana cerana Synergistic mortality Detoxification enzymes Physiological effects 

Notes

Acknowledgements

We gratefully acknowledge anonymous reviewers for their valuable comments and suggestions.

Funding

This study was found by the National Special Construction Apiculture Technology System of China (No. CARS-44-SYZ13, CARS-44-KXJ10), the State Level-scientific Institute Basal Research Fund of China (No. 2016hzs1J018; No.1630042018006) and the Youth Innovation Project of Hainan Science & Technology Association (QCXM201712).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Wensu Han
    • 1
    • 2
  • Yemeng Yang
    • 1
    • 2
  • Jinglin Gao
    • 1
    • 2
    Email author
  • Dongxiang Zhao
    • 1
    • 2
    Email author
  • Chengcai Ren
    • 3
  • Shijie Wang
    • 1
    • 2
  • Shan Zhao
    • 1
    • 2
  • Yihai Zhong
    • 1
    • 2
  1. 1.Environment and Plant Protection InstituteChinese Academy of Tropical Agricultural SciencesHaikouChina
  2. 2.Bee Industry Technology Research CenterChinese Academy of Tropical Agricultural SciencesHaikouChina
  3. 3.Hainan Bosswell Agrichemical Co., LtdHaikouChina

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