, Volume 25, Issue 10, pp 1751–1758 | Cite as

Rapid ecotoxicological bioassay using delayed fluorescence in the marine cyanobacterium Cyanobium sp. (NIES-981)

  • Takahiro Yamagishi
  • Masakazu Katsumata
  • Haruyo Yamaguchi
  • Yohei Shimura
  • Masanobu Kawachi
  • Hiroshi Koshikawa
  • Yoshifumi Horie
  • Norihisa TatarazakoEmail author


The use of delayed fluorescence intensity as an endpoint for rapid estimation of the effective concentration (ECx) has been reported as an alternative to standard growth inhibition (at 72 h after exposure) in some algal species including Pseudokirchneriella subcapitata. In marine algae, although an approach of bioassaying using delayed fluorescence measurements has not been performed yet, its development would provide many benefits for marine environmental risk assessment. In this study, we selected marine cyanobacterium Cyanobium sp. (NIES-981) as our test algal species and demonstrated that this species is valid for the standard growth inhibition test based on criteria provide by Organization for Economic Co-operation and Development guidelines. Furthermore, standard inhibition tests and shorter period test using DF were performed in NIES-981 using five chemicals (3,5-DCP, simazine, diflufenican, K2Cr2O7, and CuSO4), and their EC50 and low-toxic-effect values (EC10, EC5, and NOEC) were determined from two dose-response curves. Based on comparisons of the two dose-response curves and the EC50 values, we conclude that DF intensity is useful as an endpoint for rapid estimation of EC50 in NIES-981.


Algal growth inhibition test Cyanobium sp. Delayed fluorescence Marine cyanobacteria 



This work was partially supported by Council for Science, Technology and innovation (CSTI), Cross-ministerial Strategic Innovation Promotion Program (SIP), “Next-generation technology for ocean resources exploration”.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Research Center for Environmental RiskNational Institute for Environmental StudiesTsukubaJapan
  2. 2.Central Research LaboratoryHamamatsu Photonics K. K.HamamatsuJapan
  3. 3.Center for Environmental Biology and Ecosystem StudiesNational Institute for Environmental StudiesTsukubaJapan
  4. 4.Center for Regional Environmental ResearchNational Institute for Environmental StudiesTsukubaJapan

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