The effects of copper pyrithione, an antifouling agent, on developing zebrafish embryos
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A substitute for the organotins has been the use of metal pyrithiones, principally zinc and copper (CuPT) as antifouling agents. Zebrafish, Danio rerio, embryos were exposed after fertilization to increasing concentrations of CuPT (2, 4, 8, 12, 16, 32 and 64 μg/L) for 24 h. Morphological abnormalities at 30, 96 and 120 hours post fertilization (hpf) were recorded. Abnormalities at concentrations of 12 μg/L and higher were observed. Notochords became severely twisted as concentrations increased. These distortions of the notochord originated in the tail at the lower concentrations and proceeded rostrally with increasing dose. Edema was observed in the cardiac and yolk sac regions at the 12 and 16 μg/L CuPT concentrations. Light microscopy showed disorganization of muscle fibers, disruption and distortion of the transverse myoseptum and vacuolization of the myocyte. Hatching was measured every 12 h for 5 days following the 24 h exposure. Hatching decreased in a dose dependent manner. At 120 hpf, 47 % of the 64 μg/L CuPT treated embryos hatched. Inductively coupled plasma atomic absorbance spectrophotometry (ICPAAS) revealed copper bioaccumulation in whole embryo tissue and was significantly elevated in 32 and 64 μg/L CuPT treatment groups as compared to controls. Lipid peroxidation end products were significantly increased in animals exposed to 32 and 64 μg/L of CuPT. These data demonstrate that oxidative stress may play a role in the toxicity. The abnormalities and deformities observed in fish larvae would significantly decrease survival in polluted aqua-systems and question the use of this product as an antifouling agent.
KeywordsZebrafish Copper pyrithione Ecotoxicology Oxidative stress
Funding was provided by the Department of Pharmaceutical Sciences, College of Pharmacy, St. John’s University.
Compliance with ethical standards
Conflict of interest
The authors wish to declare that there is no conflict of interest regarding the present manuscript.
Human and Animal Rights and Informed Consent
All procedures performed in this study involving animals were in accordance with the ethical standards of the AALAC approved Animal Care Center and protocol review of the IACUC at St. John's University.
- Arch (2011) Algaecides & marine antifouling paint. http://www.archchemicals.com/Fed/BIO/Products/marinealgaecides.htm. Accessed July, 2011
- Bremner I (1998) Manifestations of copper excess. Am J Clin Nutr 67(5):1069S–1073SGoogle Scholar
- Mochida K, Ito K, Harino H, Tanaka H, Onduka T, Kakuno A, Fujii K (2009) Inhibition of acetylcholinesterase by metabolites of copper pyrithione (CuPT) and its involvement in vertebral deformity of a CuPT-exposed marine teleostean fish. Comp Physiol Biochem Part C 149(4):624–630Google Scholar
- Okamura H, Mieno H (2006) Present status of antifouling systems in Japan: tributyltin substitutes in Japan. Antifouling paint biocides. In: Konstantinou I (ed) The handbook of environmental chemistry, vol 5. Springer, Berlin, pp 201–212Google Scholar
- Sabatini S, Rocchetta I, Nahabeduan D, Luquet C, Eppis M, Bianchi L, Rios de Molina M (2011) Oxidative stress and histological alterations produced by dietary copper in the fresh water bivalve Diplodon chilensis. Comp Biochem Physiol Part C 154(4):391–398Google Scholar
- Spence R, Gerlach G, Lawrence C, Smith C (2008) The behavior and ecology of the zebrafish Danio rerio. Biol Rev 83(1):12–34Google Scholar