Ecotoxicology

, Volume 24, Issue 6, pp 1294–1304 | Cite as

Biosurfactant activity, heavy metal tolerance and characterization of Joostella strain A8 from the Mediterranean polychaete Megalomma claparedei (Gravier, 1906)

  • Carmen Rizzo
  • Luigi Michaud
  • Marco Graziano
  • Emilio De Domenico
  • Christoph Syldatk
  • Rudolf Hausmann
  • Angelina Lo Giudice
Article

Abstract

The effect of heavy metals on the activity of biosurfactants produced by Joostella strain A8 from the polychaete Megalomma claparedei was investigated. Biosurfactant activity was first improved by evaluating the influence of abiotic parameters. Higher E24 indices were achieved at 25 °C in mineral salt medium supplemented with 2 % glucose, 3 % sodium chloride (w/v) and 0.1 % ammonium chloride (w/v). Considerable surface tension reduction was never recorded. Heavy metal tolerance was preliminarily assayed by plate diffusion method resulting in the order of toxicity Cd > Cu > Zn. The activity of biosurfactants was then evaluated in the presence of heavy metals at different concentrations in liquid cultures that were incubated under optimal conditions for biosurfactant activity. The production of stable emulsions resulted generally higher in the presence of metals. These findings suggest that biosurfactant production could represent a bacterial adaptive strategy to defend cells from a stress condition derived from heavy metals in the bulk environment.

Keywords

Biosurfactant activity Filter-feeding organisms Heavy metal tolerance 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Carmen Rizzo
    • 1
  • Luigi Michaud
    • 1
  • Marco Graziano
    • 1
  • Emilio De Domenico
    • 1
  • Christoph Syldatk
    • 2
  • Rudolf Hausmann
    • 3
  • Angelina Lo Giudice
    • 1
    • 4
  1. 1.Department of Biological and Environmental SciencesUniversity of MessinaMessinaItaly
  2. 2.Institute of Process Engineering in Life Sciences, Section II: Technical BiologyKarlsruhe Institute of Technology (KIT)KarlsruheGermany
  3. 3.Institute of Food Science and Biotechnology, Section Bioprocess EngineeringUniversity of HohenheimStuttgartGermany
  4. 4.Institute for the Coastal Marine EnvironmentNational Research Council (IAMC-CNR)MessinaItaly

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