Biosurfactant activity, heavy metal tolerance and characterization of Joostella strain A8 from the Mediterranean polychaete Megalomma claparedei (Gravier, 1906)
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The effect of heavy metals on the activity of biosurfactants produced by Joostella strain A8 from the polychaete Megalomma claparedei was investigated. Biosurfactant activity was first improved by evaluating the influence of abiotic parameters. Higher E24 indices were achieved at 25 °C in mineral salt medium supplemented with 2 % glucose, 3 % sodium chloride (w/v) and 0.1 % ammonium chloride (w/v). Considerable surface tension reduction was never recorded. Heavy metal tolerance was preliminarily assayed by plate diffusion method resulting in the order of toxicity Cd > Cu > Zn. The activity of biosurfactants was then evaluated in the presence of heavy metals at different concentrations in liquid cultures that were incubated under optimal conditions for biosurfactant activity. The production of stable emulsions resulted generally higher in the presence of metals. These findings suggest that biosurfactant production could represent a bacterial adaptive strategy to defend cells from a stress condition derived from heavy metals in the bulk environment.
KeywordsBiosurfactant activity Filter-feeding organisms Heavy metal tolerance
The authors wish to thank Rossana Rando (Department of Environmental Science, Safety, Territory, of Food and Health, University of Messina) for fatty acid analysis, and Giuseppe Sabatino (Department of Earth Sciences and Physics, University of Messina) for SEM analysis.
Conflict of interest
The authors declare that they have no conflict of interest.
- Gutierrez T, Rhodes G, Mishamandani S, Berry D, Whitman WB, Nichols PD, Semple KT, Aitken MD (2014) polycyclic aromatic hydrocarbon degradation of phytoplankton-associated Arenibacter spp. and description of Arenibacter algicola sp. nov., an aromatic hydrocarbon-degrading bacterium. Appl Environ Microbiol 80:618–628CrossRefGoogle Scholar
- Hameed A, Shahina M, Lin SY, Lai WL, Liu YC, Hsu H, Cheng C, Young CC (2014) Robertkochia marina gen. nov., sp. nov., of the family Flavobacteriaceae, isolated from surface seawater, and emended descriptions of the genera Joostella and Galbibacter. IJSEM 64:533–539Google Scholar
- ISO 5509 (2000). Animal and vegetable fats and oils In: Preparation of Methyl Esters of Fatty AcidsGoogle Scholar
- Lakshmipathy TD, Prasad ASA, Kannabiran K (2010) Production of biosurfactant and heavy metal resistance activity of Streptomyces sp. VITDDK3—a novel halo tolerant actinomycetes isolated from saltpan soil. Adv Biol Res 4:108–115Google Scholar
- Satpute SK, Bhawsar BD, Dhakephalkar PK, Chopade BA (2008) Assessment of different screening methods for selecting biosurfactant producing marine bacteria. Indian J Mar Sci 37:243–250Google Scholar
- Stackebrandt E, Chertkov O, Lapidus A, Nolan M, Lucas S, Han C, Cheng J-F, Tapia R, Goodwin LA, Bruce D, Pitluck S, Liolios K, Mavromatis K, Pagani J, Ivanova N, Mikhailova N, Huntemann M, Pati A, Chen A, Palaniappan K, Rohde M, Tindall BJ, Göker M, Woyke T, Detter JC, Bristow J, Eisen JA, Markowitz V, Hugenholtz P, Klenk H-P, Kyrpides NC (2013) High-quality-draft genome sequence of the yellow-pigmented flavobacterium Joostella marina type strain (En5T). Stand Gen Sci 8:37–46CrossRefGoogle Scholar
- Tuleva B, Christova N, Jordanov B, Jordanov B, Nikolova-Damyanova B, Petrov P (2005) Naphthalene degradation and biosurfactant activity by Bacillus cereus 28BN. Z Naturforsch C 60(577):582Google Scholar