Imidacloprid induces morphological and molecular damages on testis of lizard (Podarcis sicula)
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The insecticide imidacloprid was evaluated under laboratory conditions in the adult male Italian wall lizards (Podarcis sicula) to assess its potential toxicity. By an acute oral test, LD50 was 503.76 mg/kg. Changes in spermatogenesis, plasma sex hormone levels and androgen and oestrogen receptor mRNAs were analysed by subchronic test and simulated environmental exposure. 15-days subchronic test, in which lizards were orally dosed on alternate days at 0, 10, 50, 100 mg/kg bw, showed a dose-dependent changes of testicular architecture and an increase of apoptotic processes. In a 30-days simulated environmental exposure spermatogenesis was arrested at secondary spermatocyte level and only few primary spermatocytes were TUNEL-positive. In all experimental groups imidacloprid was able to decrease both the level of sex hormones and the steroid receptor mRNAs. The results demonstrate that imidacloprid affects reproduction function of male lizards therefore precautions must be taken to minimize the harmful effects of this compound.
KeywordsImidacloprid Spermatogenesis Apoptosis Sex steroid hormones Androgen and oestrogen Receptor mRNAs Male lizard
The author wish to thank Mr. Mark Walters for linguistic revision. This work was supported by intramural funds of Biology Department to A.C.
Conflict of interest
The author declare they have no competing interests, be it financial, personal or professional.
All animals were captured with the authorization of 06/01/2000 No. SCN/2D/2000/9213 of the Italian Ministry of the Environment. The experiments were carried out in compliance with the ethical provisions enforced by the European Union and authorized by the National Committee of the Italian Ministry of Health on in vivo experimentation ( Dept. for Veterinary Public Health, Nutrition and Food Safety). All efforts were made to avoid animal suffering and to minimize the number of specimens used. The animals were maintained in accordance with the institutional guidelines for care and use of laboratory animals.
- Abou-Donia MB, Goldstein LB, Bullman S, Tu T, Khan WA, Dechkovskaia AM, Abdel-Rahman AA (2008) Imidacloprid induces neurobehavioral deficits and increases expression of glial fibrillary acidic protein in the motor cortex and hippocampus in offspring rats following in utero exposure. J Toxicol Environ Health A 71(2):119–130CrossRefGoogle Scholar
- Angelini F, Botte V (1992) Spermatogenesis in reptiles, dynamic and regulatory aspect. In: Dallai R (ed) Sex origin and evolution. Mucchi Selected Symposia and Monographs UZI, Modena, pp 211–230Google Scholar
- Bal R, Türk G, Tuzcu M, Yilmaz O, Kuloglu T, Gundogdu R, Gür S, Agca A, Ulas M, Cambay Z, Tuzcu Z, Gencoglu H, Guvenc M, Ozsahin AD, Kocaman N, Aslan A, Etem E (2012b) Assessment of imidacloprid toxicity on reproductive organ system of adult male rats. J Environ Sci Health B 47(5):434–444CrossRefGoogle Scholar
- Bro E, Decors A, Millot F, Soyez D, Moinet M, Berny P, Mastain O (2010) Intoxications des perdrix grises en nature. Nouveau bilan de la surveillance « SAGIR». Faune Sauvage 289:26–32Google Scholar
- Capula M, Aloise G (2011) Extreme feeding behaviours in the Italian wall lizard, Podarcis siculus. Acta Herpet 6(1):11–14Google Scholar
- Cardone A, Comitato R, Bellini L, Angelini F (2002) Effects of the aromatase inhibitor fadrozole on plasma sex steroid secretion and spermatogenesis, and epididymis morphology in the lizard, Podarcis sicula. Mol Reprod Dev 63:63–70Google Scholar
- Matthews J, Zacharewski T (2000) Differential binding affinities of PCBs, HO-PCBs, and aroclors with recombinant human, rainbow trout (Onchorhynkiss mykiss), and green anole (Anolis carolinensis) estrogen receptors, using a semi-high throughput competitive binding assay. Toxicol Sci 53(2):326–339CrossRefGoogle Scholar
- Najafi GR, Razi M, Hoshyar A, Shahmohamadloo S, Feyzi S (2010) The effect of chronic exposure with imidacloprid insecticide on fertility in mature male rats. Inter J Steril Fertil 4:9–16Google Scholar
- Siddiqui A, Choudhary M, Goriya HV, Bhavsar SK, Thaker AM (2007) Evaluation of immunotoxic effect of short-term administration of quinalphos and imidacloprid in white leghorn cockerels. Toxicol Int 14:15–19Google Scholar
- Todd BD, Wilson JD, Gibbons JW (2010) The global status of reptiles and causes of their decline. In: Sparling DW, Linder G, Bishop CA, Krest S (eds) Ecotoxicology of amphibians and reptiles, 2nd edn. CRC Press, Boca Raton, pp 69–104Google Scholar
- Tomlin C (2009) The pesticide manual: a world compendium, 15th edn. British Crop Protection Council, LondonGoogle Scholar
- TSK (Trimmed Spearman-Karber) program, (1991) Environmental Monitoring Systems Laboratory, US Environmental Protection Agency, Cincinnati, OH 45268, USAGoogle Scholar
- Yeh IJ, Lin TJ, Hwang DY (2010) Acute multiple organ failure with imidacloprid and alcohol ingestion. Am J Emerg Med 28(255):e1–e3Google Scholar