Toxicokinetic-toxicodynamic modelling of survival of Gammarus pulex in multiple pulse exposures to propiconazole: model assumptions, calibration data requirements and predictive power
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Toxicokinetic-toxicodynamic (TKTD) models quantify the time-course of internal concentration, which is defined by uptake, elimination and biotransformation (TK), and the processes which lead to the toxic effects (TD). TKTD models show potential in predicting pesticide effects in fluctuating concentrations, but the data requirements and validity of underlying model assumptions are not known. We calibrated TKTD models to predict survival of Gammarus pulex in propiconazole exposure and investigated the data requirements. In order to assess the need of TK in survival models, we included or excluded simulated internal concentrations based on pre-calibrated TK. Adding TK did not improve goodness of fits. Moreover, different types of calibration data could be used to model survival, which might affect model parameterization. We used two types of data for calibration: acute toxicity (standard LC50, 4 d) or pulsed toxicity data (total length 10 d). The calibration data set influenced how well the survival in the other exposure scenario was predicted (acute to pulsed scenario or vice versa). We also tested two contrasting assumptions in ecotoxicology: stochastic death and individual tolerance distribution. Neither assumption fitted to data better than the other. We observed in 10-d toxicity experiments that pulsed treatments killed more organisms than treatments with constant concentration. All treatments received the same dose, i.e. the time-weighted average concentration was equal. We studied mode of toxic action of propiconazole and it likely acts as a baseline toxicant in G. pulex during 10-days of exposure for the endpoint survival.
KeywordsOrganism recovery Delayed toxicity Dose response model Pesticide risk assessment Bioaccumulation
Toxicokinetic-toxicodynamic (TKTD) models allow predicting pesticide effects on organisms in many exposure scenarios including fluctuating or pulsed concentrations (Ashauer et al. 2006; Ashauer et al. 2007a; Ashauer and Escher 2010; Brock et al. 2010; Jager et al. 2011; Mancini 1983; Péry et al. 2001). Toxicokinetics (TK) describe processes such as uptake, distribution, biotransformation and elimination while toxicodynamics (TD) describe the processes which lead to the effects after a compound reaches the sites of toxic action (McCarty and Mackay 1993). One of the advantages of TKTD models is the ability to predict survival of organisms upon pulsed exposure, as in pesticide applications. Aquatic environments are exposed to fluctuating pesticide concentrations, not only because pesticides are applied to fields repeatedly, but also due to natural processes which are influenced by weather (e.g. frequency and intensity of rainfall events), physico–chemical properties of the compound (e.g. hydrophobicity, stability), spills and non-agricultural point sources (Kreuger 1998; Wittmer et al. 2010).
The risks posed by a contaminant on aquatic organisms are estimated by relating the predicted environmental concentrations to effect levels (Brock et al. 2010; Traas and van Leeuwen 2007). The environmental concentrations are currently predicted using fate models (FOCUS 2001) but when comparing the exposure with the effects, different parts of the fate model output can be used, e.g. time-weighted average concentration (TWA) or initial peak concentration. However, using TWA concentrations might not always be protective for the effects of pulsed exposure. Several studies have indicated that toxic effects can be more severe in exposures to short pulses than in long constant exposure with the same TWA concentration (McCahon and Pascoe 1991; Parsons and Surgeoner 1991; Schulz and Liess 2000). The predicted environmental concentrations are compared with the effect level values such as predicted no effect concentration (PNEC) or effective concentration for 50 % of individuals in a test group (EC50) (Brock et al. 2010; Traas and van Leeuwen 2007). These methods of estimating the effects are limited because the PNEC and EC50 values become meaningless in time-varying exposure patterns (Jager 2011). For example, the time which an organism needs to eliminate the substance and recover from the damage between contaminant pulses is ignored (Ashauer and Escher 2010; Jager 2011). TKTD models can simulate survival under fluctuating exposure and they take into account internal concentrations, organism damage and recovery. Therefore, they can overcome the problems related to predicted environmental concentrations, using the fate model output as it is, and predict effect levels by simulating the effects in the corresponding exposure pattern.
Still, uncertainties related to explaining and predicting the effects of pulsed exposure remain. Two assumptions, stochastic death (SD) and individual tolerance distribution (IT), have been proposed and there are no indications that only one of them would suit all combinations of chemicals and species (Ashauer and Brown 2008; Newman and McCloskey 2000; Zhao and Newman 2007). The stochastic death hypothesis assumes death to be a random process and all individuals have equal probability to die (Bedaux and Kooijman 1994; Jager et al. 2011; Newman and McCloskey 2000; Zhao and Newman 2007). The individual tolerance hypothesis, which has been dominating the ecotoxicological theory of survival, assumes that organisms have individual effect doses (Bliss 1935; Dauterman 1994; Newman and McCloskey 2000). The two hypotheses lead to different predictions of survival in subsequent pulses of equal concentrations—the SD assumption predicts equal mortality as during the previous pulse while the IT assumption predicts no mortality during the second pulse because the individuals having low thresholds for effects were eliminated during the previous pulse. The recently developed TKTD model GUTS integrates SD and IT within one model (Jager et al. 2011). This model, including the damage, damage recovery and effect threshold, was used as a basis in the current study.
Aside from the uncertainty of the assumptions underlying SD or IT when applying TKTD models in risk assessment, there is also a lack of knowledge about the data requirements for model calibration. We conducted TK experiments, a standard 4 day LC50 test (lethal concentration for 50 % of test animals) and a 10 day pulsed toxicity experiment on Gammarus pulex exposed to the fungicide propiconazole. The data were used to calibrate a set of TKTD models (Jager et al. 2011). The following model assumptions and options for calibration data were investigated: (a) how does the type of calibration data influence the parameter estimation and predictive power of the survival model, (b) how well does the survival model fit to data when the TK sub-model, simulating internal concentration, is included or excluded from the survival models, and (c) does the model assuming SD or IT better describe the data?
Materials and methods
Gammarus pulex and propiconazole
Gammarus pulex (Crustacea, Amphipoda, Gammaridae) is a key species in aquatic environments. It feeds on leaf and other organic material and therefore plays an important role in nutrient cycling (Anderson 1979). Many fish and other aquatic species feed on G. pulex and therefore G. pulex is an important part of food webs in European streams (MacNeil et al. 1997).
Propiconazole (CAS #: 60207-90-1, log Kow: 3.72) is an azole fungicide which inhibits the enzyme sterol 14α-demethylase (Zarn et al. 2002). In fungi, the enzyme inhibition interferes with biosynthesis of ergosterol, which is an essential sterol component in fungal cell membranes. In animals, the enzyme is a part of the pathway leading to biosynthesis of cholesterol, which is a component of many other sterols (Zarn et al. 2002). In arthropods, one important group of cholesterol based hormones are ecdysteroids which are involved in molting: these hormones promote the replacement of the cutile (Lafont and Mathieu 2007). Therefore, even though propiconazole is a fungicide, it might act specifically in G. pulex. We studied the mode of toxic action of propiconazole by comparing internal concentrations of propiconazole in G. pulex with internal lethal concentrations (ILC50) of known baseline toxicants in Daphnia magna (Maeder et al. 2004). If the internal concentrations in G. pulex fall into the range of ILC50 values of baseline toxicants in D. magna, propiconazole is likely to act as a baseline toxicant (i.e. acts via narcosis) in G. pulex under chosen exposure conditions.
A mixture of 14C-labelled and unlabelled propiconazole was used. The unlabelled compound (chemical purity 98.4 %) was purchased from Sigma-Aldrich and the labeled material (chemical purity 98.8 %, radiochemical purity 99.7 %) from the Institute of Isotopes Co., Ltd. Budapest, Hungary. The dosing mixture was dissolved in acetone.
Handling of Gammarus pulex and exposure
The test organisms were collected from a small headwater stream in the Itziker Ried, Switzerland (E 702150, N 2360850). To acclimatize them to laboratory conditions and equalize their nutritional status, G. pulex were maintained for 5–7 days prior to the experiments in a large aquarium in a temperature controlled room (13 °C, 12:12 light:dark photoperiod) and fed with horse chest-nut (Aesculus hippocastanum) leaves which were inoculated with the fungi Cladosporium herbarum (Naylor et al. 1989). The water in the aquarium was pre-aerated artificial pond water (APW, Table SI-1 in the Supporting Information) (Naylor et al. 1989).
In all experiments, ten test organisms were placed 1 day prior to the start of experiments in 600 mL beakers filled with 500 mL of APW. The beakers were covered with parafilm and kept in a climate chamber (13 °C, 12:12 light:dark photoperiod). The experiments started with dosing and subsequently, the water was stirred gently with a glass rod to distribute the chemical in experimental water (carrier acetone <0.2 %). Propiconazole concentration in water was measured in every beaker directly after dosing (see below). Natural mortality and mortality caused by handling of the animals were measured using non-solvent (i.e. APW only) and solvent control beakers in addition to treated beakers. Inoculated horse-chestnut leaves were provided as leaf discs with a diameter of 20 mm and five leaf discs were given to organisms in each of the beakers. Eaten leaf discs were replaced with uncontaminated discs during the experiment. The organisms were transferred to beakers containing fresh uncontaminated APW and leaf discs, either to end an exposure period or to provide fresh APW at least once in 5 days. Water pH, conductivity and oxygen concentration were measured regularly during experiments (Tables SI-2 to SI-5 in Supporting Information).
Design of TK experiments
The design of the TK experiments was based on Nuutinen et al. (2003) and Ashauer et al. (2010). Two TK experiments were conducted (TK1, TK2). Both included a 1-d exposure to propiconazole concentration of 7.8–9.5 nmol/mL which was below acute toxicity levels. After 1 day the animals were transferred to uncontaminated APW for 5-d (TK1) or 1-d (TK2). Eight replicate beakers were used, each containing ten G. pulex initially. Concentrations of propiconazole in medium were measured in every beaker (8) at different time points (TK1: 0, 5, 10, 24, 29, 34, 48, 72, 96 and 144 h and TK2: 0, 24, 29, 34 and 48 h). The average concentrations of all eight beakers per sampling time were used for modelling (Tables SI-7 and SI-9 in Supporting Information).
To determine internal concentrations of propiconazole, G. pulex samples were taken at the same time points as the water samples; except no G. pulex samples were taken at time 0 h. One G. pulex per beaker was taken each time, blotted dry with tissue paper and placed in a pre-weighed glass tube. Four G. pulex from different beakers were pooled into one sample, two pooled samples per sampling time were obtained. Pooled samples were weighed in pre-weighed glass tubes and frozen until analysis. The mean weight (±SD) of pooled samples was 84.4 ± 19.0 mg (n = 96 G. pulex, 24 pooled samples) in experiment TK1 and 108.0 ± 23.4 mg (n = 56 G. pulex, 14 pooled samples) in experiment TK2. The weight of one individual was calculated by dividing the mass of one pooled sample, containing four individuals, by four (TK 1: 21.1 ± 4.7 mg, TK 2: 27.0 ± 5.9 mg).
Design of TD experiments
Acute toxicity of propiconazole in G. pulex was measured using a standard LC50 test design. The experiment consisted of seven pesticide concentrations between 8.2 and 37.4 nmol/mL (see Table SI-14 in Supporting Information) with two replicate beakers each, each beaker containing ten G. pulex initially. Propiconazole concentrations in water were measured and the survival of G. pulex was analysed by prodding and visual observation of movements daily for 4 days.
The pulsed toxicity test lasted 10 days and consisted of three treatments. Each of the treatments had seven replicate beakers, one non-solvent and one solvent control beaker. All beakers contained ten G. pulex initially. In two of the treatments (A, B), the organisms were exposed to two 1-d pulses (concentration around LC30, 28 nmol/mL). Between pulses, the organisms had a 2-d (A) or 6-d (B) period to recover in uncontaminated APW. In the third treatment (C), the animals were exposed constantly to the same time-weighted average (TWA) concentration as in the pulsed treatments (4.6 nmol/mL). Treatment C was conducted not only to compare the toxic effects of pulsed exposure with the corresponding constant TWA concentration but also to maximize information content for calibration of the TKTD models (Albert et al. 2012). The propicanozole concentration in water was measured and the survival was observed on a daily basis.
Determination of aqueous chemical concentrations
In all experiments, aqueous concentrations were measured daily or more often (see sections above). A volume of 1 mL was sampled from experimental waters, 10 mL of Ecoscint A scintillation cocktail (Chemie Brunschwig, Switzerland) was added, samples were shaken and measured using a liquid scintillation counter (LSC, Tri-Carb 2200CA, Packard, USA). The counts were corrected for background activity by subtracting the activities in 10 mL Ecoscint A combined with 1 mL of uncontaminated experimental water (control beakers).
Determination of internal concentrations
For analysis, the frozen animals were homogenized in test tubes using a glass rod. Methanol was added twice during homogenization (1 and 2.5 mL). Then, the tubes were placed into an ultrasonic bath for 5 min and the homogenate was filtered through a 0.2 μm syringe filter (regenerated cellulose). The homogenate, syringe and filter were washed two times with methanol by vortexing. The filtrate was concentrated to a suitable volume (90 μL) using GeneVac (EZ-2 PLUS, Genevac, UK) with a method of low boiling point, 60 °C, for 50 min, and under nitrogen flow. Nanopure water was added to obtain a total volume of 300 μL to establish the appropriate methanol–water ratio for high-performance liquid chromatography (HPLC) analysis of propiconazole (see Table SI-6 in Supporting Information). The samples were split and in one aliquot the concentrations were measured using a HPLC (HP 1100, Agilent) with a radiodetector (500 TR, Packard) in order to detect both parent propiconazole and its metabolites. The other aliquot was analysed with the LSC to measure the recovery of the HPLC. The recovery was on average 93 ± 18 %. In addition, the overall recovery of the sample preparation and quantification was obtained from samples of control G. pulex spiked with known amounts of 14C labelled propiconazole. The overall recovery was 72–91 %.
Model design, formulation and description
Stochastic death models
Individual tolerance models
The likelihood function compares the observed number of death events in an observation interval with the death events predicted by the model (Jager et al. 2011). Therefore, maximising the likelihood function yields the parameter set that best describes the death events over time assuming independent death events. The log-likelihoods of the treatments were added to obtain the total likelihood. The profile of log-likelihoods was used to obtain the confidence intervals (95%) for each of the parameters (Kooijman and Bedaux 1996). Modelling procedures, including run settings and initial values, are described in more detail in the SI.
Survival curves in the pulsed exposure experiment were compared using the Kaplan–Meier log-rank test. The method uses the survival curves over time and compares them pairwise (e.g. Control vs Treatment C). It generates a p value testing the hypothesis that the survival curves are identical in the overall population.
The software GraphPad Prism 4.03 (GraphPad Software Inc., San Diego, USA) was used for determination of 1-d, 2-d, 3-d and 4-d LC50 values from acute toxicity data (Least squares optimization to sigmoidal dose–response model, top fixed at 100 % and bottom fixed at 0 %) and for comparison of the survival curves in the pulsed exposure experiment (Kaplan–Meier log-rank test). For TK and TD modelling the software ModelMaker 4 (Cherwell Scientific Ltd., Oxford, UK) was used. The maximum likelihood search was implemented by minimizing—(sum of log-likelihoods, Eq. 11). The TRACE (transparent and comprehensive ecological modelling) documentation (Schmolke et al. 2010) was followed in the modelling work and is provided in the supporting information (Box SI-1).
Estimates of toxicodynamic parameters (lower–upper 95% confidence limit) for Gammarus pulex and propiconazole according to different survival models
Mode of action of propiconazole in G. pulex
Propiconazole inhibits the enzyme sterol 14α-demethylase which is acting in the pathway that leads to biosynthesis of cholesterol in animals and might thus interfere with molting of invertebrates (Lafont and Mathieu 2007; Zarn et al. 2002). Here, the mode of toxic action of propiconazole was studied by comparing simulated internal concentrations of propiconazole in G. pulex in our toxicity tests with ILC50 of known baseline toxicants in Daphnia magna (Maeder et al. 2004). The TK model was used to simulate the internal concentrations in toxicity experiments. The concentrations were corrected by lipid content, i.e. by dividing the total concentration by the amount of lipids (G. pulex: lipid content of 1.5 %, which was measured by a gravimetric method, unpublished data; D. magna: lipid content of 1.7 % (Kretschmann et al. 2011)). The simulated internal concentrations in G. pulex reached the lower range of the baseline toxicant ILC50 of D. magna both, in the pulsed toxicity treatments, and in the two highest concentrations of the acute toxicity test (treatments A and B) (Figs. SI-5 and SI-6 in supporting information). When the lower range of baseline ILC50 was reached, mortality of 20 % (pulsed toxicity test, treatment B, concentration around LC30) to 50 % (acute toxicity test, treatments A and B) was observed in our experiments. In the TWA concentration of the pulsed toxicity experiment, the internal concentrations remained far below the ILC50 range and accordingly, the survival curve of the treatment did not differ from that of the control. Only the second pulse in both pulsed treatments caused lower mortality than 20 %, even though simulated internal concentration reached the ILC50 range of the baseline toxicants. Internal lethal concentration can be calculated as BCF × LC50 (Maeder et al. 2004; McCarty and Mackay 1993), which was 43.7 μmol/g lipid for propiconazole in G. pulex. This falls into the D. magna ILC range of baseline toxicants (Maeder et al. 2004) corrected by lipid content of 1.7 % (Kretschmann et al. 2011), 35–312 μmol/g lipid. Altogether, propiconazole seems to act as a baseline toxicant in G. pulex in 10 d exposure for the endpoint survival. However, uncertainty remains because the baseline toxicant values that we compare with are for D. magna and the internal propiconazole concentrations reached only the lower ILC50 ranges.
Effects of pulsed exposure
In our pulsed toxicity experiment, the treatments with two propiconazole pulses killed more individuals than the constant treatment with the corresponding TWA concentration. It has been hypothesised that the high exposure peaks cause higher bioaccumulation and thus more severe toxic effects than lower constant exposure with equivalent daily mean concentrations (Curtis et al. 1985; Parsons and Surgeoner 1991). This has been observed by Curtis and co-authors (1985) when they exposed early life-stage steelhead trout to fenvalerate (Curtis et al. 1985). Concentration dependent bioaccumulation (Liu et al. 2011) might also explain the results of this study, although we do not have direct evidence to support that hypothesis. However, permethrin caused more mortality to A. aegypti when the exposure was pulsed even though the exposure concentrations were equivalent. Thus, differing exposure concentrations could not explain the higher mortality by higher bioaccumulation in pulsed exposure (Parsons and Surgeoner 1991). Instead, organisms might have partially eliminated the compound and recovered between the pulses which enabled them to eat and thus take up more permethrin during the following contaminant pulse, while immobility prevented the animals from eating in constant exposure (Parsons and Surgeoner 1991; Reinert et al. 2002).
Predicted patterns of survival in multiple pulse exposures are different when based on different hypotheses of survival, stochastic death and individual tolerance (Ashauer 2010; Jager et al. 2011; Newman and McCloskey 2000; Zhao and Newman 2007). TKTD models can be used to study whether pesticide induced mortality supports IT or SD. We observed in this study with propiconazole that there appeared no clear trend between goodness of fits of SD and IT models (Fig. 4). The IT models seemed to fit better to the treatment with short recovery time between the exposure pulses while SD models described the treatment with a longer recovery period better (Fig. 3). Therefore, the hypothesis of either individual tolerance distribution or stochastic death might not solely explain the toxicity in subsequent pulses as it has been observed also by other authors (Newman and McCloskey 2000). There are other relevant processes which might affect survival (see below) and they might cause deviations from predictions provided by either of the hypothesis.
On one hand, the first pulse might weaken the surviving organisms and decrease their health, leading to increased mortality within the next pulse (Reinert et al. 2002). However, from our data we cannot infer whether the second pulse lead to “increased mortality”, because we come to opposing conclusions depending on whether we assume IT or SD (yes for IT, no for SD). In addition, our TD models would accommodate such increasing damage level. On the other hand, a first pulse might decrease mortality in the next pulse by acclimatizing the organisms to the chemical stress. For example, the first pulse might induce biotransformation and detoxification enzymes, which help the organism to deal with the subsequent pulses, and the organism might undergo some changes which alter the chemical’s target site between the pulses (Dauterman 1994; Reinert et al. 2002). Acclimatization might interfere with the two extreme hypotheses of survival and therefore neither the SD nor the IT model alone could explain the pesticide induced mortality in a pulsed exposure scenario. However, extreme cases of SD or IT could explain survival patterns in several studies. For example, a pesticide inhibiting acetylcholine esterase, diazinon, has shown clear stochastic death patterns in G. pulex (Ashauer et al. 2010). In addition, data of mosquitofish exposed to sodium chloride or pentachlorophenol pulses supported that the stochastic component determined fish survival (Newman and McCloskey 2000). On the other hand, the support for individual tolerance theory originates in observations that insects after few generations seemed to achieve resistance to herbicides and insecticides (Bliss 1935; Dauterman 1994). Mode of toxic action as well as species characteristics might also affect the applicability of the stochastic death or individual tolerance hypothesis.
Organism recovery times (95% of recovery) based on different model types
95% recovery times (days)
Organism recovery can be driven either by TK (i.e. elimination) or TD (i.e. damage recovery). Propiconazole was eliminated shortly after transfer to uncontaminated water (95 % elimination time ≈ 10 h). When comparing the TK and TD recovery parameters kout and kd, the TD recovery rate constant kd was lower than the elimination rate constant kout according to almost all models (Table 1). Therefore, it can be concluded that TD recovery dominated overall organism recovery. Ashauer et al. (2010) came to the same conclusion when they exposed G. pulex to diazinon. The opposite has been observed by Ashauer et al. (2007b) when G. pulex were exposed to carbaryl: the elimination rate constant is 0.27 (1/d) while TD recovery rate constant is 0.97 (1/d) (Ashauer et al. 2007b), although this observation must be revised in light of new insights into biotransformation, also of carbaryl, in G. pulex (Ashauer et al. 2012). Ashauer et al. (2007b) measured only total radioactivity, not biotransformation. When biotransformation to naphthol-sulphate is also considered, the elimination rate of carbaryl is 2.3 (1/day) and the total loss rate for carbaryl is 5.6 (1/d) (Ashauer et al. 2012). Thus, the organism recovery of G. pulex exposed to carbaryl is also dominated by toxicodynamics.
Previously it has been claimed that TK is an essential part of understanding survival patterns over time (Ashauer et al. 2010; Butcher et al. 2006), which might suggest that TK may also be essential for predicting survival over time. Here we compared the goodness of fit between reduced and full models (Fig. 4). The difference in model structure between the full and reduced models is that the full model includes a pre-calibrated TK sub-model which simulates internal concentrations in the different exposure scenarios (Fig. 1). This is used as an input to the survival model, where it scales the organism damage. The reduced model skips the internal concentration and damage steps but instead, the internal concentration is scaled and can be described as a lumped variable for damage and internal concentration. In almost all cases, the reduced models resulted in the same or even higher log-likelihood than the full models. This can be explained by variation which the TK experiment brings to the survival model because the TK and TD experiments were not conducted simultaneously. One can conclude that in this example, measuring and simulating TK was not essential to achieve good predictions of survival. However, even if not necessary in survival models, TK provide important information on compound bioaccumulation potential and biotransformation, as well as mode of action, and therefore should not be disregarded.
TKTD models show potential to predict effects of multiple pulse exposure. The models have been calibrated using long term pulsed toxicity experiments (Ashauer et al. 2007a, b; Ashauer et al. 2010; Butcher et al. 2006; Meyer et al. 1995) or constant exposure experiments (Mancini 1983; Meyer et al. 1995). Using the toxicity data from constant exposures for model calibration would allow applying these models widely in risk assessment because this type of data has been and is generated in standard toxicity experiments. Here, we calibrated the TKTD models using pulsed toxicity data, acute toxicity data or both together. Therefore we could compare the goodness of fit and parameter estimates amongst calibration data sets. In addition, we were able to use parameters produced by either of the data sets to predict the effects in the other scenario and compare the simulation results with observations (model validation, see TRACE in Box SI-1). The calibration data had an influence on the parameter estimates (Table 1) and on the goodness of fit (Fig. 4). As expected, overall fit was the best if both data sets were used for model calibration, i.e. the total MPE was the lowest. In addition, the total likelihood (shown in Fig. 4 as combined likelihood value for acute and pulsed toxicity data above each model type) was maximised by fitting the models to both data sets together. When only one data set was used for the calibration, the choice of calibration data affected the predictive power of the model. If we compare the total likelihood values of the models calibrated with either pulsed toxicity or acute toxicity data, we see that the maximum likelihood is achieved using acute toxicity data for calibration. Similarly, previous studies have shown that the effects of time-varying exposure can be rather well predicted based on constant acute toxicity data (Mancini 1983; Meyer et al. 1995) but the effects of constant acute toxicity exposure are poorly predicted with time-varying toxicity data (Meyer et al. 1995). Meyer et al. (1995) stated that either the models did not mimic the processes well enough or there are different physiological processes which determine the toxicity under constant and time-varying exposure. Thus, the effects of time-varying exposure might be better predicted using data from time-varying exposure under different exposure regimes than using data from constant exposure studies (Meyer et al. 1995).
The choice of calibration data also had an effect on how the model predicts survival in a different exposure scenario. For example, the model calibrated using pulsed toxicity data mostly underestimated mortality in the acute toxicity scenario (Fig. 4). On the other hand, when acute toxicity data alone was used to calibrate the models, mortality in the pulsed exposure scenario was overestimated. In a risk assessment context, this overestimated mortality in the prediction could be acceptable. Thus one could calibrate TKTD models using already existing acute toxicity data because they provide protective predictions of survival in a pulsed (natural) exposure scenario. However, we here studied only one combination of chemical and organism, which is not sufficient to generalize this conclusion. More evidence is required before recommendations for appropriate model calibration data or model structures (e.g. with or without TK) can be made. Therefore studies on TKTD models and how they are able to predict time-varying exposure using acute or pulsed toxicity data should be conducted using more combinations of chemicals and species.
This research has been financially supported by the European Union under the 7th Framework Programme (project acronym CREAM, contract number PITN-GA-2009-238148).
Conflict of interest
The authors declare that they have no conflict of interest.
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