, Volume 21, Issue 3, pp 662–666 | Cite as

Embryotoxicity of Corexit 9500 in mallard ducks (Anas platyrhynchos)

  • Kimberly J. Wooten
  • Bryson E. Finch
  • Philip N. Smith


Embryotoxicity of the oil dispersant Corexit 9500 was examined using fertilized mallard duck eggs. Corexit 9500 was topically applied below the air cell to eggs in volumes ranging from 0 to 100 μL on day 3 of incubation. The highest incidence of mortality occurred at developmental stage 4, one day post-Corexit 9500 application. Hatching success was significantly decreased among eggs treated with ≥20 μL of Corexit 9500 as compared to controls (P ≤ 0.047). No egg treated with ≥40 μL successfully hatched. The application volume resulting in 50% mortality (corrected for control survival) was determined to be 15.5 μL. Developmental stage at embryo death was also significantly decreased compared to controls in eggs exposed to 40 μL (P = 0.0042) and above.


Corexit 9500 Mallard duck Anasplatyrhynchos Dispersant Oil spill 


  1. Albers PH (1977) Effects of external applications of fuel oil on hatchability of mallard eggs. In: Wolfe DA (ed) Fate and effects of petroleum hydrocarbons in marine ecosystems. Pergamon Press, New York, pp 158–163Google Scholar
  2. Albers P (1979) Effects of Corexit 9527 on the hatchability of mallard eggs. Bull Environm Contam Toxicol 23:661–668CrossRefGoogle Scholar
  3. Albers PH, Gay ML (1982) Effects of a chemical dispersant and crude oil on breeding ducks. Bull Environm Contam Toxicol 29:404–411CrossRefGoogle Scholar
  4. Caldwell PJ, Snart AE (1974) A photographic index for aging mallard embryos. J Wildl Manage 38:298–301CrossRefGoogle Scholar
  5. Cornell Lab of Ornithology, American Ornithologist Union (2011) The birds of North America online database. Cornell Lab of Ornithology, American Ornithologist Union, Ithaca, NYGoogle Scholar
  6. Finch BE, Wooten KJ, Smith PN (2011) Embryotoxicity of weathered crude oil from the Gulf of Mexico in mallard ducks (Anas platyrhynchos). Environ Toxicol Chem 30(8):1885–1891CrossRefGoogle Scholar
  7. George-Ares A, Clark JR (2000) Aquatic toxicity of two Corexit(R) dispersants. Chemosphere 40:897–906CrossRefGoogle Scholar
  8. Hemmer MJ, Barron MG, Greene RM (2010a) Comparative toxicity of eight oil dispersant products on two Gulf of Mexico aquatic test species.
  9. Hemmer MJ, Barron MG, Greene RM (2010b) Comparative toxicity of Louisiana sweet crude (LSC) and chemically dispersed LSC to two Gulf of Mexico aquatic test species.
  10. Hoffman DJ, Albers PH (1984) Evaluation of potential embryotoxicity and teratogenicity of 42 herbicides, insecticides, and petroleum contaminants to mallard eggs. Arch Environ Contam Toxicol 13:15–27CrossRefGoogle Scholar
  11. Kujawinski EB, Kido Soule MC, Valentine DL, Boysen AK, Longnecker K, Redmond MC (2011) Fate of dispersants associated with the Deepwater Horizon oil spill. Environ Sci Technol 45(4):1298–1306CrossRefGoogle Scholar
  12. Metzer Farms (2011) Incubating and Hatching of Duck Eggs. Accessed 24 Jan 2011
  13. Milinkovitch T, Kanan R, Thomas-Guyon H, Le Floch S (2011) Effects of dispersed oil exposure on the bioaccumulation of polycyclic aromatic hydrocarbons and the mortality of juvenile Liza ramada. Sci Total Environ 409:1643–1650CrossRefGoogle Scholar
  14. NALCO Company (2010) Corexit ingredients. Accessed 24 Jan 2011
  15. National Research Council (NRC) (2005) Oil spill dispersants: efficacy and effects. National Academy Press, WashingtonGoogle Scholar
  16. Operational Science Advisory Team (OSAT) (2010) Summary report for sub-sea and sub-surface oil and dispersant detection: sampling and monitoring. United Area Command, Dec 17, 2010. Accessed 11 Feb 2011
  17. Place B, Anderson B, Mekebri A, Furlong ET, Gray JL, Tjeerdema R, Field J (2010) A role for analytical chemistry in advancing our understanding of the occurrence, fate, and effects of Corexit oil dispersants. Environ Sci Technol 44:6016–6018CrossRefGoogle Scholar
  18. Prince HH, Siegel PB, Cornwell GW (1969) Incubation environment and the development of mallard embryos. J Wildl Manage 33:589–595CrossRefGoogle Scholar
  19. R Core Development Team (2010) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna. ISBN 3-900051-07-0. Available online:
  20. Ramachandran SD, Hodson PV, Khan CW, Lee K (2004) Oil dispersant increases PAH uptake by fish exposed to crude oil. Ecotoxicol Environ Saf 59:300–308CrossRefGoogle Scholar
  21. Regional Response Team 4 (RRT-4) (1996) Use of dispersants in region IV.$file/1-RRT4DISP.PDF. Accessed 20 Jun 2011
  22. Regional Response Team 6 (RRT-6) (2001) FOSC dispersant pre-approval guidelines and checklist. Accessed 15 Feb 2011
  23. Rittinghaus H (1956) On the indirect spread of oil pollution in a seabird sanctuary. Ornithol Mitt 8:43–46Google Scholar
  24. Singer MM, George S, Jacobson S, Lee I, Weetman LL, Tjeerdma RS, Sowby ML (1996) Comparison of acute aquatic effects of the oil dispersant Corexit 9500 with those of other Corexit series dispersants. Ecotoxicol Environ Saf 35(2):183–189CrossRefGoogle Scholar
  25. Stubblefield WA, Toll PA (1993) Effects of incubation-temperature and warm-water misting on hatching success in artificially incubated mallard duck eggs. Environ Toxicol Chem 12:695–700CrossRefGoogle Scholar
  26. US Fish and Wildlife Service (USFWS) (2011) Bird impact data from doi-erdc database download 14 Dec. 2010. Accessed 17 Mar 2011

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Kimberly J. Wooten
    • 1
  • Bryson E. Finch
    • 1
  • Philip N. Smith
    • 1
  1. 1.Department of Environmental ToxicologyThe Institute of Environmental and Human Health, Texas Tech UniversityLubbockUSA

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