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Ecotoxicology

, Volume 20, Issue 5, pp 993–999 | Cite as

Hydroxyl radical generation and oxidative stress in earthworms (Eisenia fetida) exposed to decabromodiphenyl ether (BDE-209)

  • Xianchuan Xie
  • Yingxin Wu
  • Mengying Zhu
  • You-kuan Zhang
  • Xiaorong Wang
Article

Abstract

Antioxidant responses induced by decabromodiphenyl ether (BDE-209) in the earthworms (Eisenia fetida) were studied after 7 days of exposure. Electron paramagnetic resonance (EPR) spectra indicated that hydroxyl radicals (•OH) in earthworms were significantly induced by 0.01–10 mg/kg of BDE-209. Malondialdehyde (MDA) and protein carbonyl (PCO) were stimulated at 0.5–10 mg/kg and 1–10 mg/kg, respectively. The reduced glutathione (GSH) was inhibited at 1–10 mg/kg while oxidized glutathione (GSSG) accumulated at 0.5–10 mg/kg. The GSH/GSSG ratio decreased at 0.5–10 mg/kg, and superoxide dismutase (SOD) and glutathione-S-transferase (GST) activities were induced at 0.1–1 and 0.5 mg/kg, respectively and both decreased at 10 mg/kg. Catalase (CAT) activities increased at 1–10 mg/kg. The results showed that severe oxidative stress occurred in E. fetida, and may play an important role in inducing the toxicity of BDE-209 on earthworms.

Keywords

Decabromodiphenyl ether BDE-209 Oxidative stress Hydroxyl radical (•OH) Earthworm 

Notes

Acknowledgments

This study was funded by the National Basic Research Program of China (973 program, No. 2009CB421604), China-EU International S&T Cooperation Program (No.0911) and the State Key Laboratory Program of Pollution Control and Resource Reuse (Nanjing University, PR China). The authors thank Yunxia Sui for her technical help with analysis of EPR spectra. The authors also wish to thank Dr Jing Wang for her kind assistance.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Xianchuan Xie
    • 1
  • Yingxin Wu
    • 1
  • Mengying Zhu
    • 1
  • You-kuan Zhang
    • 1
  • Xiaorong Wang
    • 1
  1. 1.State Key Laboratory of Pollution Control and Resource Reuse, Center for Hydrosciences Research, School of the EnvironmentNanjing UniversityNanjingPeople’s Republuic of China

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