Ecotoxicology

, Volume 20, Issue 2, pp 438–446

Biodegradation of malachite green by Pseudomonas sp. strain DY1 under aerobic condition: characteristics, degradation products, enzyme analysis and phytotoxicity

Article

Abstract

Malachite green (MG), a widely-used and recalcitrant dye, has been confirmed to be carcinogenic and mutagenic against many organisms. The main objective of this study is to investigate the capability of Pseudomonas sp. strain DY1 to decolorize MG, and to explore the possible mechanism. The results showed that this strain demonstrated high decolorizing capability (90.3–97.2%) at high concentrations of MG (100–1,000 mg/l) under shaking condition within 24 h. In static conditions, lower but still effective decolorization (78.9–84.3%) was achieved. The optimal pH and temperature for the decolorization was pH 6.6 and 28–30°C, respectively. Mg2+ and Mn2+ (1 mM) were observed to significantly enhance the decolorization. The intermediates of the MG degradation under aerobic condition identified by UV–visible, GC–MS and LC–MS analysis included malachite green carbinol, (dimethyl amino-phenyl)-phenyl-methanone, N,N-dimethylaniline, (methyl amino-phenyl)-phenyl-methanone, (amino phenyl)-phenyl methanone and di-benzyl methane. The enzyme analysis indicated that Mn-peroxidase, NADH–DCIP and MG reductase were involved in the biodegradation of MG. Moreover, phytotoxicity of MG and detoxification for MG by the strain were observed. Therefore, this strain could be potentially used for bioremediation of MG.

Keywords

Biodegradation Pseudomonas sp. Malachite green Degradation products Enzyme analysis Phytotoxicity 

References

  1. Ayed L, Chaieb K, Cheref A, Bakhrouf A (2009) Biodegradation of triphenylmethane dye malachite green by Sphingomonas paucimobilis. World J Microbiol Biotechnol 25:705–711CrossRefGoogle Scholar
  2. Bermek H, Gülseren I, Li K, Jung H, Tamerler C (2004) The effect of fungal morphology on ligninolytic enzyme production by a recently isolated wood-degrading fungus Trichophyton rubrum LSK-27. World J Microbiol Biotechnol 20:345–349CrossRefGoogle Scholar
  3. Chen CY, Kuo JT, Cheng CY, Huang YT, Ho IH, Chung YC (2009) Biological decolorization of dye solution containing malachite green by Pandoraea pulmonicola YC32 using a batch and continuous system. J Hazard Mater 172:1439–1445CrossRefGoogle Scholar
  4. Chen CH, Chang CF, Liu SM (2010) Partial degradation mechanisms of malachite green and methyl violet B by Shewanella decolorationis NTOU1 under anaerobic conditions. J Hazard Mater 177:281–289CrossRefGoogle Scholar
  5. Chhabra M, Mishra M, Sreekrishnan TR (2009) Laccase/mediator assisted degradation of triarylmethane dyes in a continuous membrane reactor. J Biotechnol 143:69–78CrossRefGoogle Scholar
  6. Daneshvar N, Khataee AR, Rasoulifard MH, Pourhassan M (2007) Biodegradation of dye solution containing malachite green: optimization of effective parameters using Taguchi method. J Hazard Mater 143:214–219CrossRefGoogle Scholar
  7. Deng D, Guo J, Zeng G, Sun G (2008) Decolorization of anthraquinone, triphenylmethane and azo dyes by a new isolated Bacillus cereus strain DC11. Int Biodeterior Biodegrad 62:263–269CrossRefGoogle Scholar
  8. Fessard VT, Godard T, Huet S, Mourot A, Poul JM (1999) Mutagenicity of malachite green and leucomalachite green in vitro tests. J Appl Toxicol 19:421–430CrossRefGoogle Scholar
  9. Forgacs E, Cserháti T, Oros G (2004) Removal of synthetic dyes from wastewaters: a review. Environ Int 30:953–971CrossRefGoogle Scholar
  10. Hameed BH, Lee TW (2009) Degradation of malachite green in aqueous solution by Fenton process. J Hazard Mater 164:468–472CrossRefGoogle Scholar
  11. Han R, Wang Y, Sun Q, Wang L, Song J, He X, Dou C (2010) Malachite green adsorption onto natural zeolite and reuse by microwave irradiation. J Hazard Mater 175:1056–1061CrossRefGoogle Scholar
  12. Jadhav JP, Govindwar SP (2006) Biotransformation of malachite green by Saccharomyces cerevisiae MTCC 463. Yeast 23:315–323CrossRefGoogle Scholar
  13. Kalyani DC, Patil PS, Jadhav JP, Govindwar SP (2008) Biodegradation of reactive textile dye Red BLI by an isolated bacterium Pseudomonas sp. SUK1. Bioresour Technol 99:4635–4641CrossRefGoogle Scholar
  14. Kammoun MM, Mechichi HZ, Belbahri L, Woodward S, Mechichi T (2009) Malachite green decolourization and detoxification by the laccase from a newly isolated strain of Trametes sp. Int Biodeterior Biodegrad 63:600–606CrossRefGoogle Scholar
  15. Li LT, Hong Q, Yan X, Fang GH, Ali SW, Li SP (2009) Isolation of a malachite green-degrading Pseudomonas sp. MDB-1 strain and cloning of the tmr2 gene. Biodegradation 20:769–776CrossRefGoogle Scholar
  16. Papinutti L, Mouso N, Forchiassin F (2006) Removal and degradation of the fungicide dye malachite green from aqueous solution using the system wheat bran–Fomes sclerodermeus. Enzyme Microb Technol 39:848–853CrossRefGoogle Scholar
  17. Parshetti G, Kalme S, Saratale G, Govindwar S (2006) Biodegradation of malachite green by Kocuria rosea MTCC 1532. Acta Chim Slov 53:492–498Google Scholar
  18. Rahman IA, Saad B, Shaidan S, Sya Rizal ES (2005) Adsorption characteristics of malachite green on activated carbon derived from rice husks produced by chemical–thermal process. Bioresour Technol 96:1578–1583CrossRefGoogle Scholar
  19. Sani RK, Banerjee UC (1999) Decolorization of triphenylmethane dyes and textile and dye-stuff effluent by Kurthia sp. Enzyme Microb Technol 24:433–437CrossRefGoogle Scholar
  20. Shedbalkar U, Dhanve R, Jadhav J (2008) Biodegradation of triphenylmethane dye cotton blue by Penicillium ochrochloron MTCC 517. J Hazard Mater 157:472–479CrossRefGoogle Scholar
  21. Srivastava S, Sinha R, Roy D (2004) Toxicological effects of malachite green. Aquat Toxicol 66:319–329CrossRefGoogle Scholar
  22. Tien M, Kirk TK (1988) Lignin peroxidase of Phanerochaete chrysosporium. Methods Enzymol 161:238–249CrossRefGoogle Scholar
  23. Van der Zee FP, Villaverde S (2005) Combined anaerobic–aerobic treatment of azo dyes—a short review of bioreactor studies. Water Res 39:1425–1440CrossRefGoogle Scholar
  24. Wu J, Jung BG, Kim K-S, Lee Y-C, Sung N-C (2009) Isolation and characterization of Pseudomonas otitidis WL-13 and its capacity to decolorize triphenylmethane dyes. J Environ Sci 21:960–964CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.College of Life ScienceZhejiang UniversityHangzhouPeople’s Republic of China
  2. 2.Department of Agriculture and BiotechnologyWenzhou Vocational College of Science and TechnologyWenzhouPeople’s Republic of China

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