, Volume 20, Issue 1, pp 274–282 | Cite as

B-esterase activities and blood cell morphology in the frog Leptodactylus chaquensis (Amphibia: Leptodactylidae) on rice agroecosystems from Santa Fe Province (Argentina)

  • Andrés M. AttademoEmail author
  • Mariana Cabagna-Zenklusen
  • Rafael C. Lajmanovich
  • Paola M. Peltzer
  • Celina Junges
  • Agustín Bassó


Activity of B-esterases (BChE: butyrylcholinesterase and CbE: carboxylesterase using two model substrates: α-naphthyl acetate and 4-nitrophenyl valerate) in a native frog, Leptodactylus chaquensis from rice fields (RF1: methamidophos and RF2: cypermethrin and endosulfan sprayed by aircraft) and non-contaminated area (pristine forest) was measured. The ability of pyridine-2-aldoxime methochloride (2-PAM) to reactivate BChE levels was also explored. In addition, changes in blood cell morphology and parasite infection were determined. Mean values of plasma BChE activities were lower in samples from the two rice fields than in those from the reference site. CbE (4-nitrophenyl valerate) levels varied in the three sites studied, being highest in RF1. Frog plasma from RF1 showed positive reactivation of BChE activity after incubation with 2-PAM. Blood parameters of frogs from RF2 revealed morphological alterations (anisochromasia and immature erythrocytes frequency). Moreover, a major infection of protozoan Trypanosoma sp. in individuals from the two rice fields was detected. We suggest that integrated use of several biomarkers (BChE and CBEs, chemical reactivation of plasma with 2-PAM, and blood cell parameters) may be a promising procedure for use in biomonitoring programmes to diagnose pesticide exposure of wild populations of this frog and other native anuran species in Argentina.


Frog Leptodactylus chaquensis B-esterase Blood cell morphology Rice fields Pesticides 



We wish to thank Mateo R. and Chuiko G. for critical comments and valuable suggestions that improved the manuscript. We thank Elberg G. for help during sample collection and farmers for permission to work in their rice fields. This work was supported by PICT-SECYT No. 1148 (Director: Dr. Rafael C. Lajmanovich).


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Andrés M. Attademo
    • 1
    • 2
    Email author
  • Mariana Cabagna-Zenklusen
    • 1
  • Rafael C. Lajmanovich
    • 1
    • 2
  • Paola M. Peltzer
    • 1
    • 2
  • Celina Junges
    • 1
    • 2
  • Agustín Bassó
    • 1
  1. 1.Laboratory of Ecotoxicology, Faculty of Biochemistry and Biological Sciences (ESS-FBCB-UNL)Santa FeArgentina
  2. 2.National Council for Scientific and Technical Research (CONICET), ESS-FBCB-UNLSanta FeArgentina

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