, Volume 17, Issue 3, pp 189–198 | Cite as

Effects of nickel and temperature on the ground beetle Pterostichus oblongopunctatus (Coleoptera: Carabidae)

  • Agnieszka J. BednarskaEmail author
  • Ryszard Laskowski


In natural ecosystems it is not unusual for an organism to be exposed both to chemical and physical stressful factors at the same time. Herein we present results of the study on nickel toxicity to the carabid beetle, Pterostichus oblongopunctatus, and effect of Ni and temperature on the beetles respiration rates. In the first part of the study (Experiment I) we measured the survival, respiration rates and internal Ni concentrations in animals exposed for 245 d at constant temperature (20 °C) to food contaminated with Ni at nominal concentrations 0; 600; 1,200; 2,400; 4,800; and 9,600 mg kg−1 dry weigh (dw). The LC50 was estimated at 8,351 mg Ni kg−1, with no effect on fertility. We found a significant positive correlation between Ni concentration in food and internal body concentration of Ni, and a negative correlation between Ni exposure and the respiration rate. Based on these results, the concentration of 2,400 mg kg−1 (LOEC for the respiration rate) was selected for the second part of the study (Experiment II) in which field-collected males of P. oblongopunctatus were exposed to Ni-contaminated food for 64 d and then to uncontaminated food for the next 64 d at three temperatures: 10, 15 and 20°C. In this part of the study we found that the temperature under which the beetles were kept affected their respiration rates, and that effect of Ni on the respiration was significant only in animals originating from 20°C. The results from both experiments indicate that negative effects of nickel appear only after relatively long exposure.


Nickel Temperature Respiration rate Ground beetles 



We are grateful to Ms. Renata Śliwińska for her help in the field and laboratory. We also would like to thank the Olkusz Forest Service for a kind cooperation. The study was supported by the EU-Integrated project NoMiracle (Novel Methods for Integrated Risk assessment of Cumulative Stressors in Europe;; contract No. 003956 under the EU-theme “Global Change and Ecosystems” topic “Development of risk assessment methodologies”, coordinated by Dr. Hans Løkke at NERI, DK-8600 Silkeborg, Denmark), the Polish Ministry of Science and Informatization (contract No. 158/E-338/6. PR UE/DIE 279/2005–2008), and the Jagiellonian University (DS-758).


  1. Abdel-Lateif HM, Donker MH, van Straalen NM (1998) Interaction between temperature and cadmium toxicity in the isopod Porcelio scaber. Funct Ecol 12:521–527CrossRefGoogle Scholar
  2. Bednarska A, Portka I, Kramarz P, Laskowski R (2006) Combined effects of nickel, chlorpyrifos and temperature on life-history traits of Pterostichus oblongopunctatus (Coleoptera: Carabidae) In: Book of abstracts of International Conference on Ecotoxicology: Trends and Perspectives, Wisła, Poland, p 19Google Scholar
  3. Boyd RS, Davis MA, Wall MA, Balkwill K (2007) Host-herbivore studies of Stenoscepa sp. (Orthroptera: Pyrgomorphidae), a high-Ni herbivore of the South African Ni hyperaccumulator Berkheya cossii (Asteraceae). Insect Sci 14:133–143CrossRefGoogle Scholar
  4. Brunsting AMH (1981) Distribution patterns, life cycle and phenology of Pterostichus oblongopunctatus F. (Col., Carabidae) and Philonthus decorus grav. (Col., Staphylinidae). Neth J Zool 31:418–452CrossRefGoogle Scholar
  5. Calow P (1991) Physiological costs of combating chemical toxicants: ecological implications. Comp Biochem Physiol C 100:3–6CrossRefGoogle Scholar
  6. Chaabane K, Josens G, Loreau M (1999) Respiration of Abax ater (Coleoptera, Carabidae): a complex parameter of the energy budget. Pedobiologia 43:305–318Google Scholar
  7. Eisler R (1998) Nickel hazards to fish, wildlife, and invertebrates: a synoptic review. Contaminant Hazard Reviews. Report No. 34. Patuxent Wildlife Research Center, U. S. Geologicla Survey, Laurel, MD 20708Google Scholar
  8. Ermler U, Grabarse W, Shima S, Goubeaud M, Thauer R (1998) Active sites of transition-metal enzymes with focus on nickel. Curr Opin Struct Biol 8:749–758CrossRefGoogle Scholar
  9. Everhart JL, McNear D, Peltier E, van der Lelie D, Chaney RL, Sparks DL (2006) Assesing nickel bioavailability in smelter-contaminated soils. Sci Total Environ 367:732–744CrossRefGoogle Scholar
  10. Handy RD, Depledge MH (1999) Physiological responses: their measurements and use as environmental biomarkers in ecotoxicology. Ecotoxicology 8:329–349CrossRefGoogle Scholar
  11. Holmstrup M, Bayley M, Sjursen H, Hřjer R, Bossen S, Friis K (2000) Interactions between environmental pollution and cold tolerance of soil invertebrates: a neglected field of research. CryoLetters 21:309–314Google Scholar
  12. Hopkin SP (1989) Ecophysiology of metals in invertebrates. Elsevier, Applied Science, London, EnglandGoogle Scholar
  13. Janssen MPM, Bruins A, de Vries TH, van Straalen NM (1991) Comparison of cadmium kinetics in four soil arthropod species. Arch Environ Contam Toxicol 20:305–312CrossRefGoogle Scholar
  14. Kabata-Pendias A (2000) Trace elements in soils and plants. 3rd edn, CRC Press IncGoogle Scholar
  15. Kaplan EL, Meier P (1958) Non-parametric estimation from incomplete observations. J Am Stat Assoc 70:865–871Google Scholar
  16. Kramarz P (1999) Dynamics of accumulation and decontamination of cadmium and zinc in carnivorous invertebrates. 1. The ground beetle, Poecilus cupreus L. Bull Environ Contam Toxicol 63:531–537CrossRefGoogle Scholar
  17. Laskowski R (2001) Why short-term bioassays are not meaningful – effects of a pesticide (imidacloprid) and a metal (cadmium) on pea aphids (Acyrthosiphon pisum Harris). Ecotoxicology 10:177–183CrossRefGoogle Scholar
  18. Laskowski R., Maryański M (1993) Heavy-metals in epigeic fauna – trophic-level and physiological hypotheses. Bull Environ Contam Toxicol 50:232–240CrossRefGoogle Scholar
  19. Laskowski R, Maryański M, Pyza E, Wojtusiak J (1996) Sublethal toxicity tests for long-lived invertebrates: searching for a solution. In: Van Straalen NM, Krivolutsky DA (eds) Bioindicator systems for soil pollution NATO ASI Series, 2: environment. Kluwer, Dordrecht/Boston/London, pp 45–55Google Scholar
  20. Lock K, Janssen CR (2002) Ecotoxicity of nickel to Eisenia fetida, Enchytraeeus albidus and Folsomia candida. Chemosphere 46:197–200CrossRefGoogle Scholar
  21. Løkke H (1995) Effects of pesticides on meso- and microfauna in soil. Bekaempelsesmiddelforskning fra Miljøstyrelsen 8, Danish Environmental Protection Agency, DenmarkGoogle Scholar
  22. Łagisz M, Kramarz P, Niklińska M (2005) Metal kinetics and respiration rates in F1 generation of carabid beetles (Pterostichus oblongopunctatus F.) originating from metal-contaminated and reference areas. Arch Environ Contam Toxicol 48:484–489CrossRefGoogle Scholar
  23. Łagisz M, Laskowski R (2002) Respiratory metabolism in Pterostichus oblongopunctatus originating from metal contaminated and reference areas. Fresenius Environ Bull 11:74–77Google Scholar
  24. Łagisz M, Laskowski R, Kramarz P, Tobor M (2002) Population parameters of the beetle Pterostichus oblongopunctatus F. from metal contaminated and reference areas. Bull Environ Contam Toxicol 69:243–249CrossRefGoogle Scholar
  25. Łukasik P, Laskowski R (2007) Increased respiration rate as a result of adaptation to copper in confused flour beetle, Tribolium confusum Jacquelin du Val. Bull Environ Contam Toxicol 79:311–314CrossRefGoogle Scholar
  26. Migula P (1989) Combined and separate effects of cadmium, lead and zinc on respiratory metabolism during the last larval stage of the house cricket, Acheta domesticus. Biologia (Bratislava) 44:513–521Google Scholar
  27. Potvin C, Roff DA (1993) Distribution-free and robust statistical methods – Viable alternatives to parametric statistics. Ecology 73:910–926Google Scholar
  28. Przybyłowicz WJ, Mesjasz-Przybyłowicz J, Migula P, Głowacka E, Nakonieczny M, Augustyniak M (2002) Micro-PIXE elemental mapping of the gut and malpighian tubules of the beetle, Chrysolina pardalina. Proc 15th ICEM-Durban: 695–696Google Scholar
  29. Rowe CL, Hopkins WA, Zehnder C, Congdon JD (2001) Metabolic costs incurred by crayfish (Procambarus acutus) in a trace element-polluted habitat: further evidence of similar responses among diverse taxonomic groups. Comp Biochem Physiol C 129:275–283Google Scholar
  30. Scott-Fordsmand JJ, Krogh PH, Hopkin SP (1999) Toxicity of nickel to a soil-dwelling springtail, Folsomia fimetaria (Collembola: Isotomidae). Ecotoxicol Environ Saf 43:57–61CrossRefGoogle Scholar
  31. Scott-Fordsmand JJ, Weeks JM, Hopkin SP (1998) Toxicity of nickel to the earthworm and the applicability of the neutral red retention assay. Ecotoxicology 7:219–295CrossRefGoogle Scholar
  32. Sibly RM, Calow P (1989) A life-cycle theory of responses to stress. Biol J Linn Soc 37:101–116CrossRefGoogle Scholar
  33. Stefanowicz AM, Niklińska N, Laskowski R (2008) Metals affect soil bacterial and fungal functional diversity differently. Environ Toxicol Chem 27(3), doi:10.1897/07-288. Google Scholar
  34. Stone D, Jepson P, Kramarz P, Laskowski R (2001) Time to death response in carabid beetles exposed to multiple stressors along a gradient of heavy metal pollution. Environ Pollut 113:239–244CrossRefGoogle Scholar
  35. Walker CH, Hopkin SP, Sibly RM, Peakall DB (2006) Principles of Ecotoxicology. 3rd edn, Taylor and FrancisGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  1. 1.Institute of Environmental SciencesJagiellonian UniversityKrakowPoland

Personalised recommendations