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Environmental Biology of Fishes

, Volume 102, Issue 2, pp 221–232 | Cite as

Bonefish (Albula vulpes) oocyte lipid class and fatty acid composition related to their development

  • Sahar MejriEmail author
  • Cameron Luck
  • Rejean Tremblay
  • Marty Riche
  • Aaron Adams
  • Matthew J. Ajemian
  • Jonathan Shenker
  • Paul S. Wills
Article

Abstract

Bonefish (Albula vulpes) are a valuable fishery resource of tropical and subtropical ecosystems worldwide. Despite their importance, there is limited information on bonefish life history and ecology. The present study aims to describe, for the first time, oocytes development and their lipid characteristics in wild bonefish during the reproductive season in different tidal flat locations in Grand Bahama Island, Bahamas. Our results have shown that Bonefish follow group-synchronous ovarian development and produce lipid-rich eggs [total lipid (TL) content was >26% of wet mass (WM)]. The major lipid class was a neutral lipid: the wax esters and steryl esters (WE-SE; >48% of TL), suggesting their use to support buoyancy and/or energy storage. Fatty acid (FA) composition of bonefish oocytes was characterized by high levels of monoenes in the neutral lipid fraction (i.e. 16:1 and 18:1n-9) indicating their probable role as energy fuel. However, the most abundant fatty acids in the polar lipids were docosahexaenoic acid (DHA; 22:6 n-3 > 15% of total polar FA), eicosapentaenoic acid (EPA; 20:5 n-3 > 13% of total polar FA) and arachidonic acid (ARA; 20:4 n-6 > 4% of total polar FA) which were selectively conserved among the tidal flat locations, suggesting their importance as essential constituents of cell membranes during the development of bonefish oocytes. Our results bring useful information concerning the reproductive physiology of bonefish and not only serve as a benchmark for determining the nutrient requirements to produce high quality eggs from bonefish captive broodstock, but also will help establish meaningful management practices for this species.

Keywords

Bonefish Oocytes Lipid Fatty acid Wax esters and steryl esters 

Abbreviations

ALC

fatty alcohol

ARA

arachidonic acid

BB

Barbary beach

CA

cortical alveolus

CBE

Crabbing Bay east

DHA

docosahexaenoic acid

EFA

essential fatty acids

EPA

eicosapentaenoic acid

FA

fatty acid

FFA

free fatty acids

HC

hydrocarbon

KET

ketones

LV

late vitellogenic

MUFA

monunsaturated fatty acids

PL

phospholipids

PG

primary growth

PUFA

polyunsaturated fatty acids

SDWC

South Deep Water Caye

SFA

saturated fatty acids

ST

sterols

TAG

triacylglycerol

TL

total lipids

WE-SE

wax ester - steryl ester

WM

wet mass

Notes

Acknowledgements

This study was financially supported by Bonefish & Tarpon Trust (BTT) and National Fish and Wildlife Foundation (NFWF). We are grateful to J. Lewis, W. Halstead, Z. Nilles, and C. Robinson for their extensive help during fieldwork and lab work.

Compliance with ethical standards

Ethical approval

The experimental protocol received approval from the Florida Atlantic University’s Institutional Animal Care and use Committee (IACUC, protocol A16–34).

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Sahar Mejri
    • 1
    Email author
  • Cameron Luck
    • 1
  • Rejean Tremblay
    • 2
  • Marty Riche
    • 1
  • Aaron Adams
    • 1
    • 3
  • Matthew J. Ajemian
    • 1
  • Jonathan Shenker
    • 4
  • Paul S. Wills
    • 1
  1. 1.Harbor Branch Oceanographic InstituteFlorida Atlantic UniversityFort PierceUSA
  2. 2.Institut des Sciences de la MerUniversité du Québec à Rimouski (ISMER, UQAR)RimouskiCanada
  3. 3.Bonefish and Tarpon TrustCoral GablesUSA
  4. 4.Florida Institute of TechnologyMelbourneUSA

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