The effect of rearing temperature on body shape and meristic characters in zebrafish (Danio rerio) juveniles
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Although zebrafish (Danio rerio) is a highly studied organism on many fields of research, many aspects of its basic biology still elude the scientific community. Its response to temperature - especially developmental one - has been very scarcely studied and this is an important lack of knowledge since the species is considered quite eurythermal in nature. In the present study, zebrafish was subjected to four different developmental temperatures (22, 25, 28 and 31°C) from the half-epiboly stage until after metamorphosis in order to examine whether the temperature can influence the juvenile’s phenotype. Morphometric and meristic characters were explored. Body shape and almost all of the meristic characters studied were significantly affected by the temperature applied during the first stages of development. Most meristic characters of the study, presented a significant differentiation in the extreme temperatures used (22 and/or 31°C), whereas lower temperatures seemed to produce higher meristic counts in the majority of the characters. Zebrafish juveniles, as shown in this study, exhibit highly variable phenotypes (phenotypic plasticity) induced by diverse thermal conditions during their early ontogenetic stages possibly in order to successfully adjust to different environments.
KeywordsTemperature Zebrafish Meristic Body shape Morphology Larval rearing
The authors thank two anonymous reviewers for their helpful comments in producing the final manuscript. The present study was financed by the European Social Fund and National Resources (EPEAEK II–PYTHAGORAS I) to M.K.
- Axelrod H, Schultz L (1990) Handbook of tropical aquarium fishes. McGraw-Hill, New York, p 718Google Scholar
- Froese R, Pauly D (2009) FishBase website, http://www.fishbase.org/Summary/SpeciesSummary.php?ID=4653. Accessed at 7 June 2010
- Harder W (1975) Anatomy of fishes. Schweizerbart, StuttgartGoogle Scholar
- Ibañez-Aguirre AL, Lleonart J (1996) Relative growth and comparative morphometrics of Mugil cephalus L. and M. curema V. in the Gulf of Mexico. Sci Mar 60(2–3):361–368Google Scholar
- Johnston IA, Lee HT, Macqueen DJ, Paranthaman K, Kawashima C, Anwar A, Kinghorn JR, Dalmay T (2009) Embryonic temperature affects muscle fibre recruitment in adult zebrafish: genome-wide changes in gene and microRNA expression associated with the transition from hyperplastic to hypertrophic growth phenotypes. J Exp Biol 212(12):1781PubMedCrossRefGoogle Scholar
- Matsuoka M (1987) Development of the skeletal tissues and skeletal muscles in the red sea bream (Pagrus major). Bull Seikai Reg Fish Res Lab 65:1–114Google Scholar
- Sfakianakis DG, Koumoundouros G, Divanach P, Kentouri M (2004) Osteological development of the vertebral column and of the fins in Pagellus erythrinus (L. 1758). Temperature effect on the developmental plasticity and morpho-anatomical abnormalities. Aquaculture 232(1–4):407–424CrossRefGoogle Scholar
- Sokal R, Rohlf F (1995) Biometry: the principles and practice of statistics in biological research. WH Freeman, New YorkGoogle Scholar
- Taylor WR, van Dyke GC (1985) Revised procedures for staining and clearing small fishes and other vertebrates for bone and cartilage study. Cybium 9(2):107–119Google Scholar
- Westerfield M (1995) The zebrafish book: a guide for the laboratory use of zebrafish (Danio rerio). University of Oregon Press, EugeneGoogle Scholar
- Wilkes D, Xie SQ, Stickland NC, Alami-Durante H, Kentouri M, Sterioti A, Koumoundouros G, Fauconneau B, Goldspink G (2001) Temperature and myogenic factor transcript levels during early development determines muscle growth potential in rainbow trout (Oncorhynchus mykiss) and sea bass (Dicentrarchus labrax). J Exp Biol 204(16):2763–2771PubMedGoogle Scholar