Environmental Biology of Fishes

, Volume 85, Issue 3, pp 201–206 | Cite as

Analysis of genetic diversity in Glyptosternum maculatum (Sisoridae, Siluriformes) populations with AFLP markers

  • Baoying Guo
  • Congxin Xie
  • Shunping He
  • Khalid Abbas
  • Huanling Wang
  • Dongmei Xiong
  • Huijuan Zhang
  • Xin Tong
Article

Abstract

We determined the genetic diversity of geographic populations from three spawning grounds (Nyang River, Lhasa River, Shetongmon Reach of Yarlung Zangbo River) of Glyptosternum maculatum with amplified fragment length polymorphism (AFLP) markers. Five primer combinations detected 332 products, 51 of them (15.4%) were polymorphic in at least one population. The Shetongmon population was found to be the richest in genetic diversity as was indicated by the percentage of polymorphic loci and heterozygosity, followed by the Nyang population and the Lhasa population. The pair-wise genetic distance between populations were all very close, ranging from 0.0015 to 0.0042 with an average of 0.0024. The genetic distance was not proportional to the geographic distance. The analysis of molecular variance demonstrated that all variation occurred within populations. The average estimated fixation index (Fst) of three populations across all polymorphic loci was −0.0184, indicating the absence of genetic differences among the three sampled populations. The differentiation among populations was not significant, and population structure was weak. Our observations will help identify the genetic relationship among populations as the first approach to understand the genetic diversity of Glyptosternum maculatum.

Keywords

AFLP Genetic diversity Glyptosternum maculatum 

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Baoying Guo
    • 1
  • Congxin Xie
    • 1
  • Shunping He
    • 2
  • Khalid Abbas
    • 1
  • Huanling Wang
    • 1
  • Dongmei Xiong
    • 1
  • Huijuan Zhang
    • 1
  • Xin Tong
    • 1
  1. 1.College of FisheriesHuazhong Agriculture UniversityWuhanChina
  2. 2.Laboratory of Evolutionary Biology and Biogeography for Freshwater Fishes, Institute of HydrobiologyThe Chinese Academy of SciencesWuhanPeople’s Republic of China

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