Environmental Biology of Fishes

, Volume 75, Issue 4, pp 455–463 | Cite as

Genetic Structure and Low-genetic Diversity Suggesting the Necessity for Conservation of the Chinese Longsnout Catfish, Leiocassis longirostris (Pisces: Bagriidae)

  • Zhongwei Wang
  • Jianfeng Zhou
  • Yuzhen Ye
  • Qiwei Wei
  • Qingjiang Wu


Genetic variation and phylogenetic relationship of Leiocassis longirostris populations from the Yangtze River were investigated at mitochondrial DNA level. The samples were collected from the upstream and mid-downstream of the Yangtze River. Three mitochondrial DNA fragments, ND5/6, cytochrome b (Cyt b) and control region (D-loop), were amplified and then digested by 10 restriction endonucleases. Twenty-three D-loop fragments randomly selected were sequenced. Digestion patterns of ND5/6 by AluI and HaeIII, D-loop by HinfI and RsaI, and Cyt b by HaeIII were polymorphic. Ten and eighteen haplotypes were obtained from RFLP data and sequence data, respectively. The individuals from upstream and mid-downstream of the Yangtze River were apparently divided into two groups. The average genetic distance was 0.008 and 0.010 according to the two data. Low diversities and decreasing abundance indicated that Leiocassis longirostris may be in severe danger and reasonable measures of fishery management should be taken.


Leiocassis longirostris mitochondrial DNA genetic diversity PCR–RFLP 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Aurelle, D., Berrebi, P 2001Genetic structure of brown trout (Salmo trutta, L.) populations from south-western France: data from mitochondrial control region variabilityMol. Ecol.1015511561CrossRefGoogle Scholar
  2. Bernardi, G., Crane, N.L 1999Molecular phylogeny of the humbug damselfishes inferred from mtDNA sequencesJ. Fish Biol.5412101217CrossRefGoogle Scholar
  3. Carr, M.C., Marshall, H.D 1991Detection of intraspecific DNA sequence variation in the mitochondrial cytochrome b gene of Atlantic cod (Gadus morhua) by the polymerase chain reactionCan. J. Fish. Aquat. Sci.484852CrossRefGoogle Scholar
  4. Dai, J.H., Yin, W.L., Yang, D.S., Xiong, Q.M 1994Studies on mitochondrial DNA from liver of Leiocassis longirostrisJ. Wuhan Univ.1115120(in Chinese)Google Scholar
  5. Excoffier, L., Smouth, P.E., Quattro, J.M 1992Analysis of molecular variance inferred from metric distance among DNA haplotypes: application to human mitochondrial DNA restriction dataGenetics131479491Google Scholar
  6. Fu, C.Z., Wu, J.H., Chen, J.K., Wu, Q.H., Lei, G.C 2003Freshwater fish biodiversity in the Yangtze River basin of China: patterns, threats and conservationBiodivers. Conserv.1216491685CrossRefGoogle Scholar
  7. Hansen, M.M., Mensberg, K-LD, Berg, S 1999Postglacial recolonization patterns and genetic relationships among whitefish (Coregonus sp.) populations in Denmark, inferred from mitochondrial DNA and microsatellite markersMol. Ecol.8239252CrossRefGoogle Scholar
  8. Hoelzel, A.R., Dahlheim, M., Stern,  1998Low genetic variation among killer whales (Orcinus orca) in the eastern north Pacific and genetic differentiation between foraging specialistsJ. Hered.89121128CrossRefGoogle Scholar
  9. Kumar, S., Tamura, K., Jakobsen, I.B., Nei, M 2001MEGA2: molecular evolutionary genetics analysis softwareBioinformatics1712441245CrossRefGoogle Scholar
  10. Lindak, P., Paul, M 1994Developments in molecular genetic techniques in fisheriesRev. Fish Biol. Fish.4272299CrossRefGoogle Scholar
  11. Liu, H.Z., Tzeng, C.S., Teng, H.Y 2002Sequence variations in the mitochondrial DNA control region and their implications for the phylogeny of the CypriniformesCan. J. Zool.80569581CrossRefGoogle Scholar
  12. Lu, G.Q., Li, S.F., Bernatchez, L 1997Mitochondrial DNA diversity, population structure and conservation genetics of four carps within the Yangtze River, ChinaCan. J. Fish. Aquat. Sci.544758CrossRefGoogle Scholar
  13. Luo, M., Jiang, L.K., Liu, Y., Zhan, G.Q., Xia, S.Z. 2000Comparative study on isoenzymes in Leiocassis longirostrisChin. J. Appl. Environ. Biol.6447451(in Chinese)Google Scholar
  14. McElroy, D.P., Moran, P., Bermingham, E., Kornfield, I 1992REAP: the restriction analysis packageJ. Hered.862227Google Scholar
  15. Nei, M., Li, W.H. 1979Mathematical model for studying genetic variation in terms of restriction endonucleasesProc. Natl. Acad. Sci.7652695273CrossRefGoogle Scholar
  16. Nesbo, C.L., Arab, M.O., Jakobsen, K.S 1998Heteroplasmy, length and sequence variation in the mtDNA control regions of three percid fish species (Perca fluviatilis, Acerina cernua, Stizostedion lucioperca)Genetics14819071919Google Scholar
  17. Park, L.K., Brainard, M.A., Dightman, D.A., Winans, G.A 1993Low levels of intraspecific variation in the mitochondrial DNA of chum salmo (Onchorhynchus keta)Mol. Mar. Biol. Biotechnol.2362370Google Scholar
  18. Poteaux, C., Berrebi, P., Bomhomme, F 2001Allozymes, mtDNA and microsatellites study introgression in a stocked trout population in FranceRev. Fish Biol. Fish.10281292CrossRefGoogle Scholar
  19. Sambrook, J.Fritsch, E.F.Maniatis ,  T. eds. 1989Molecular cloning: a laboratory manual2Cold Spring Harbor Laboratory PressNew YorkGoogle Scholar
  20. Schneider S., D. Roessli & L. Excoffier. 2000. Arlequin: a software for population genetics data analysis. Version 2.000. Genetics and Biometry Lab, Dept. of Anthropology, University of GenevaGoogle Scholar
  21. Simons, A.M., Wood, R.M., Heath, L.S., Kuhajda, B.R., Mayden,  R.L. 2001Phylogentics of Scaphirhynchus based on mitochondrial DNA sequencesTrans. Am. Fish. Soc.130359366CrossRefGoogle Scholar
  22. Szalanski, A.L., Bischof, R., Mestl, G 2000Population genetic structure of Nebraska paddlefish based on mitochondrial DNA variationTrans. Am. Fish. Soc.12910601065CrossRefGoogle Scholar
  23. Takeyama, H., Chow, S., Tsuzuki, H., Matsunaga, T 2001Mitochondrial DNA sequence variation within and between tuna Thunnus species and its application to species identificationJ. Fish Biol.5816461657CrossRefGoogle Scholar
  24. Tang, K.C. 2001An atlas of neotectonics geological hazards and quaternary glacial geomorphy in the Yangtze Three Gorges AreaHubei Science and Techology PressWuhan, P.R. China(in Chinese)Google Scholar
  25. Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F., Higgins, D.G 1997The Clustal_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis toolsNucleic Acids Res.2548764882CrossRefGoogle Scholar
  26. Toline, C.A., Baker, A.J 1995Mitochondrial DNA variation and population genetic structure of the northern redbelly dace (Phoxinus eos)Mol. Ecol.4745753Google Scholar
  27. Triantafyllidis, A., Abatzopoulos, T.J., Economidis, P.S 1999Genetic differentiation and phylogenetic relationships among greek Silurus glanis and Silurus aristotelis (Pisces, Siluridae) populations, assessed by PCR–RFLP analysis of mitochondrial DNA segmentsHeredity82503509CrossRefGoogle Scholar
  28. Ward, R.D., Woodwark, M., Skibinski, D.O.F 1994A comparison of genetic diversity levels in marine, freshwater and anadromous fishesJ. Fish Biol.44213232CrossRefGoogle Scholar
  29. Wu, C.J 1975Population ecology of [Leiocassis longirostris (Gunther)] (Pisces, Bagridae) with reference to the problem of maximum sustained yieldActa Hydrobiol. Sin.5387409(in Chinese)Google Scholar
  30. Wu, J.M., Jiang, R.L., Zhang, J.P 1999Artificial induced spawning of Leiocassis longirostris and change of ultrastructure of hypophysis GTH cellJ. Fish. China23248253(in Chinese)Google Scholar
  31. Xiao, W.H., Zhang, Y.P., Liu, H.Z. 2001Molecular systematics of Xenocyprinae (Teleostei: Cyprinidae): taxonomy, biogeography, and coevolution of a special group restricted in East AsiaMol. Phylogenet. Evol.18163173CrossRefGoogle Scholar
  32. Zhang, H., Mikawa, N., Yamada, Y., Horie, N., Okamura, A., Utoh,  T., Tanaka, S 1999Foreign eel species in the natural waters of Japan detected by polymerase chain reaction of mitochondrial cytochrome b regionFish. Sci.65684686Google Scholar
  33. Zhang S.M. 1998. Molecular population genetics of Chinese sturgeon with reference to cellular and molecular evolution of some species of Acipenseriformes. PhD Thesis, Wuhan, Institute of Hydrobiology, Chinese Academy of Sciences (in Chinese)Google Scholar
  34. Zhang, S.M., Deng, H., Wang, D.Q., Yu, L.N 2001Population structure and genetic diversity of silver carp and grass carp from populations of Yangtze River system revealed by RAPDActa Hydrobiol. Sin.25324330(in Chinese)Google Scholar

Copyright information

© Springer 2006

Authors and Affiliations

  • Zhongwei Wang
    • 1
    • 2
  • Jianfeng Zhou
    • 1
    • 2
  • Yuzhen Ye
    • 1
  • Qiwei Wei
    • 3
    • 4
  • Qingjiang Wu
    • 1
  1. 1.State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of HydrobiologyChinese Academy of SciencesWuhanP.R. China
  2. 2.Graduate School of Chinese Academy of SciencesBeijingChina
  3. 3.Ministry of AgricultureThe Key Lab of Freshwater Fish Germplasm and BiotechnologyP.R. China
  4. 4.Yangtze River Fisheries Research InstituteChinese Academy of Fisheries SciencesJingzhou, HubeiP.R. China

Personalised recommendations