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Matteucinol, isolated from Miconia chamissois, induces apoptosis in human glioblastoma lines via the intrinsic pathway and inhibits angiogenesis and tumor growth in vivo

  • Ana Gabriela Silva
  • Viviane Aline O. Silva
  • Renato J. S. Oliveira
  • Allisson Rodrigues de Rezende
  • Rafael César Russo Chagas
  • Lúcia Pinheiro Santos Pimenta
  • Wanderson Romão
  • Hélio Batista Santos
  • Ralph Gruppi Thomé
  • Rui  Manuel ReisEmail author
  • Rosy Iara Maciel de Azambuja RibeiroEmail author
PRECLINICAL STUDIES
  • 61 Downloads

Summary

Gliomas account for nearly 70% of the central nervous system tumors and present a median survival of approximately 12–17 months. Studies have shown that administration of novel natural antineoplastic agents is been highly effective for treating gliomas. This study was conducted to investigate the antitumor potential (in vitro and in vivo) of Miconia chamissois Naudin for treating glioblastomas. We investigated the cytotoxicity of the chloroform partition and its sub-fraction in glioblastoma cell lines (GAMG and U251MG) and one normal cell line of astrocytes. The fraction showed cytotoxicity and was selective for tumor cells. Characterization of this fraction revealed a single compound, Matteucinol, which was first identified in the species M. chamissois. Matteucinol promoted cell death via intrinsic apoptosis in the adult glioblastoma lines. In addition, Matteucinol significantly reduced the migration, invasion, and clonogenicity of the tumor cells. Notably, it also reduced tumor growth and angiogenesis in vivo. Moreover, this agent showed synergistic effects with temozolomide, a chemotherapeutic agent commonly used in clinical practice. Our study demonstrates that Matteucinol from M chamissois is a promising compound for the treatment of glioblastomas and may be used along with the existing chemotherapeutic agents for more effective treatment.

Keywords

Central nervous system cancer Melastomataceae Chicken chorioallantoic membrane assay DNA damage Resonance mass spectrometry Antitumor 

Notes

Funding

The work was supported by Minas Gerais State Research Foundation (FAPEMIG), National Council for Scientific and Technological Development (CNPq),and supported partially by grantsfrom the FINEP (MCTI/ FINEP/MS/SCTIE/DECIT-01/2013 - FPXII-BIOPLAT). This work was conducted during a scholarship supported by the International Cooperation Program CAPES (Brazilian Federal Agency for Support and Evaluation of Graduate Education within the Ministry of Education of Brazil) at the Federal University of São João del Rei.

Compliance with ethical standards

Conflict of interest

Author Silva, A.G. declares that she has no conflict of interest. Author Saito, V.A.O.S. declares that she has no conflict of interest. Author Oliveira R.J.S. declares that he has no conflict of interest. Author Rezende, A.R. declares that he has no conflict of interest. Author Chagas R.C.R. declares that he has no conflict of interest. Author Pimenta, L.P.S. declares that she has no conflict of interest. Author Romão, W. declares that he has no conflict of interest. Author Santos, H.B. declares that he has no conflict of interest. Author Thomé R.G. declares that he has no conflict of interest. Author Reis, R. declares that he has no conflict of interest. Author Ribeiro, R.I.M.A. declares that she has no conflict of interest.

Ethical approval

This article has a study with animals, ethical approval was obtained by the Ethics Committee of the Foundation Pio XII IRCAD Brazil under protocol number 027/2016. All applicable international, national, institutional guidelines for the care and use of animals were followed.

Supplementary material

10637_2019_878_MOESM1_ESM.pdf (250 kb)
Table S1 (PDF 249 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Experimental Pathology LaboratoryFederal University of São João del ReiDivinópolisBrazil
  2. 2.Molecular Oncology Research CenterBarretos Cancer HospitalBarretosBrazil
  3. 3.State University of Minas GeraisItuiutabaBrazil
  4. 4.Department of Chemistry, Institute of Exact SciencesFederal University of Minas GeraisBelo HorizonteBrazil
  5. 5.Petroleomic and Forensic Laboratory, Chemistry DepartmentFederal University of Espírito SantoVitóriaBrazil
  6. 6.Federal Institute of EducationScience and Technology of Espírito SantoVila VelhaBrazil
  7. 7.Tissue Processing LaboratoryFederal University of São João del Rei (UFSJ)DivinópolisBrazil
  8. 8.Life and Health Sciences Research Institute (ICVS), School of MedicineUniversity of MinhoBragaPortugal
  9. 9.ICVS/3B’s - PT Government Associate LaboratoryBraga/GuimarãesPortugal

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