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Novel palladium (II) complexes with tetradentate thiosemicarbazones. Synthesis, characterization, in vitro cytotoxicity and xanthine oxidase inhibition

  • Dilşad Özerkan
  • Onur Ertik
  • Buşra Kaya
  • Serap Erdem KurucaEmail author
  • Refiye Yanardag
  • Bahri Ülküseven
PRECLINICAL STUDIES
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Summary

In vitro cytotoxicity and xanthine oxidase inhibition capabilities were investigated for five palladium (II) chelate complexes. The palladium complexes were synthesized by starting from S-alkyl-thiosemicarbazones where the alkyl component is methyl, ethyl, propyl or butyl. The solid complexes are characterized by elemental analysis and spectroscopic techniques (UV-visible, IR and 1H NMR). In order to be able to verify the N2O2-type thiosemicarbazidato ligand (L2−) structure in the square planar geometry, complex 1 has been studied as a representative by using single crystal X-ray crystallography. The in vitro cytotoxic activity measurements were carried out in HepG2 and Hep3B hepatocellular carcinomas, HCT116 colorectal carcinoma, and 3 T3 mouse fibroblast cell lines. The palladium complexes exhibited notable cytotoxic activities in all cell lines at lower μM concentrations compared to the standard chemicals, cisplatin and allopurinol. IC50 values were determined between 0.42 ± 0.01 and 12.01 ± 0.37 μg/ml in examining the antixanthine oxidase abilities of the complexes. Two complexes with S-methyl group exhibited a high inhibition activity on the xanthine oxidase. The results indicated that these complexes could be used as active pharmaceutical ingredients.

Keywords

Palladium Thiosemicarbazone X-ray analysis Cytotoxicity Xanthine oxidase 

Notes

Acknowledgements

Supplementary Material Crystallographic data for the structural analysis have been deposited with the Cambridge Crystallographic Data Centre, CCDC No. CCDC 1411477 for complex 1.

We thankful to Ekin Altepe for proofreading.

Funding

This study was funded by The Scientific Research Projects Coordination Unit of Istanbul University, Project Number 54237.

Compliance with ethical standards

Conflict of interest

All authors declare no conflicts of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Dilşad Özerkan
    • 1
  • Onur Ertik
    • 2
  • Buşra Kaya
    • 2
  • Serap Erdem Kuruca
    • 3
    Email author
  • Refiye Yanardag
    • 2
  • Bahri Ülküseven
    • 2
  1. 1.Department of Genetic and Bioengineering, Faculty of Engineering and ArchitectureKastamonu UniversityKastamonuTurkey
  2. 2.Department of Chemistry, Engineering FacultyIstanbul University-CerrahpasaIstanbulTurkey
  3. 3.Deparment of Physiology, Istanbul Medical FacultyIstanbul UniversityIstanbulTurkey

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