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Nifuroxazide induces apoptosis, inhibits cell migration and invasion in osteosarcoma

  • Yi Luo
  • Anqi Zeng
  • Aiping Fang
  • Linjiang Song
  • Chen Fan
  • Chenjuan Zeng
  • Tinghong Ye
  • Hao Chen
  • Chongqi Tu
  • Yongmei XieEmail author
PRECLINICAL STUDIES
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Summary

Osteosarcoma is the most common primary malignancy of bone and characterized by an appendicular primary tumor with a high rate of metastasis to the lungs. Unfortunately, there is no effective strategy to treat osteosarcoma in current clinical practice. In this study, the anticancer effects and potential mechanisms of nifuroxazide, an oral nitrofuran antibiotic, on two osteosarcoma cell lines were investigated. The results of the antiproliferative activity in vitro showed that nifuroxazide inhibited cell proliferation of UMR106 and MG63 cells in a dose- and time-dependent manner. Interestingly, nifuroxazide showed low toxicity to non-tumor cells (HEK 293 T). In addition, ROS-mitochondrial mediated apoptosis was observed after treatment of nifuroxazide. Moreover, nifuroxazide could significantly inhibit osteosarcoma cells migration and invasion via p-Stat3, MMP-2 and MMP-9 mediated signaling pathway. Taken together, our results suggested that nifuroxazide could be a promising agent for osteosarcoma treatment by inhibiting cell proliferation, inducing cell apoptosis and impairing cell migration and invasion.

Keywords

Osteosarcoma Nifuroxazide Apoptosis Anti-proliferation Anti-metastasis 
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Notes

Funding

This work was supported by the Support Program for Science and Technology of Sichuan Province (2017SZ0106).

Compliance with ethical standards

Conflict of interest

The authors confirm that they have no conflict of interest with the content of this article.

Ethical approval

This article does not contain studies with human participants or animals performed by any of the authors.

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Orthopedics, West China HospitalSichuan UniversityChengduPeople’s Republic of China
  2. 2.State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, and Collaborative Innovation Center for BiotherapySichuan UniversityChengduPeople’s Republic of China
  3. 3.Sichuan Academy of Chinese Medicine SciencesChengduPeople’s Republic of China
  4. 4.School of Medical and Life SciencesChengdu University of Traditional Chinese MedicineChengduPeople’s Republic of China
  5. 5.Department of Pharmaceutical Sciences, College of PharmacyUniversity of Tennessee Healthy Science CenterMemphisUSA

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