ISCEV extended protocol for the S-cone ERG


The International Society for Clinical Electrophysiology of Vision (ISCEV) standard for full-field electroretinography (ERG) describes a minimum procedure for testing generalized retinal function but encourages more extensive testing. This extended protocol describes a method of assessing the function of the short-wavelength-sensitive cone (S-cone) retinal pathway, using a short-wavelength flash superimposed on a background that saturates the rods and adapts the L/M-cones to elicit a response, known as the S-cone ERG. Stimulus parameters such as the strength and luminance of the flash and background, respectively, and their spectral and temporal characteristics are specified. As a complement to the ISCEV standard, testing the S-cone ERG enables further characterization of light-adapted retinal function and may refine diagnosis of some retinal disorders. Typical applications are described including use in the diagnosis of rod monochromacy and S-cone monochromacy, identification and investigation of cone On-bipolar cell dysfunction and use of the technique to confirm the diagnosis of enhanced S-cone syndrome.


The International Society for Clinical Electrophysiology of Vision (ISCEV) standard for full-field electroretinography (ERG) describes a minimum procedure for testing generalized retinal function but encourages more extensive testing [1]. This extended protocol describes a method of assessing the function of the short-wavelength-sensitive cone (S-cone) retinal pathway, using a short-wavelength flash superimposed on a background light to elicit a response, known as the S-cone ERG. The S-cone ERG is a specialized procedure which is well established and broadly accepted by experts in the field. The protocol was prepared by the authors in accordance with ISCEV procedures ( and approved by the ISCEV Board of Directors on September 23rd, 2019.

Scope and application

The ISCEV standard ERGs are recorded under dark-adapted (DA) and light-adapted (LA) conditions to assess both rod and cone system functions. The standard LA ERGs are normally dominated by responses driven by the long-wavelength-sensitive cones (L-cones) and medium-wavelength-sensitive cones (M-cones). There are far fewer short-wavelength-sensitive cones (S-cones) than L/M-cones, and their summed responses to light are of smaller amplitude and slower kinetics. Therefore, the S-cone-mediated retinal activity is not directly visible in the standard LA ERGs. This extended protocol describes parameters for the recording of the S-cone ERG, reflecting the activity of the S-cone pathways in the distal retina. The S-cone ERG is elicited using short-wavelength (blue) flashes on a long-wavelength background to saturate the rod system and to reduce contributions from the L/M-cone systems [2,3,4]. The ERGs to such stimuli normally vary in waveform shape according to flash strength. To dim stimuli, there is typically a single low-amplitude component of relatively long peak time (Fig. 1). To stronger flashes, an additional earlier peak occurs and grows with increasing flash strength. The action spectrum of the 1st peak has a maximum at 520 nm and that of the 2nd peak at 450 nm [3]. In S-cone monochromacy, there is a later peak only [3], whereas in tritanopia there may be a normal 1st peak and a very small 2nd peak [4]. These and other studies are consistent with response contributions from the L/M-cone system to the first peak and the S-cone system to the second peak.

Fig. 1

Stimulus–response series of the S-cone ERG recorded from a control subject using a Burian–Allen corneal electrode. An LED system was used to generate a 1 ms blue (450 nm half-height bandwidth 20 nm) full-field flash, superimposed on a yellow (570 nm) background (luminance 300 cd m−2). The stimulus frequency was 4.5 Hz. In this case, 300–500 responses were averaged. At low stimulus strengths (approximately of 0.01–0.02 cd s m−2), single positive S-cone responses are observed. At moderate and high stimulus strengths (approximately 0.025–0.12 cd s m−2), two positive peaks are seen, the first (at approx. 30 ms) reflects activity of the L/M-cone system and the second (at approx. 40 ms) is the S-cone-mediated ERG component. Red dotted lines are drawn at 40 ms

The addition of S-cone ERG testing to the standard ERG protocol enables further characterization of LA retinal function and can help in determining the origin of abnormal standard ERGs. Typical clinical applications of S-cone ERGs are outlined below:

  1. a.

    In inherited retinal disorders, a more detailed knowledge of the phenotype may refine the diagnosis and facilitate mutational screening. It can confirm the origins of the recorded signals in enhanced S-cone syndrome (ESCS) consequent upon mutation in NR2E3 gene after the pathognomonic features have been revealed with conventional ISCEV standard ERG testing [5, 6]. Mutations in the NRL gene, the upstream gene to NR2E3, also cause a retinal phenotype similar to ESCS, but not identical in all ERG features [7, 8].

  2. b.

    S-cone ERG recording is important in the distinction between rod monochromacy and S-cone monochromacy. In both disorders, the rod-driven ERG components are normal or well-preserved. The LA 3.0 ERG is usually undetectable in rod monochromacy, but in S-cone monochromacy may be detectable as a small amplitude b-wave of long implicit time appearing at approximately 50 ms, suggestive of an S-cone system origin that can be more accurately confirmed by S-cone ERG recording [2]. S-cones, like rods, transmit signals via an On-bipolar cell pathway, and any disorder affecting global retinal On-pathway function, such as “complete” CSNB (congenital stationary night blindness), will have reduced S-cone ERGs [9].

  3. c.

    Inherited tritanopia is rare, and such patients cannot be identified with Ishihara plates, but only by testing color vision in the tritan color confusion axis (e.g., HRR plates, Farnsworth-Munsell 100-Hue, L’Anthony D15, color contrast sensitivity, etc.). Although many patients with tritanopic defects may have normal S-cone ERGs, some patients show S-cone ERG abnormalities [4].

  4. d.

    Elevated tritan thresholds can also occur in acquired disorders associated with reduction in S-cone pathway function. Examples include melanoma-associated retinopathy, characterized by ERGs indistinguishable from those in complete congenital stationary night blindness, and reflecting On-bipolar cell dysfunction. Tritan defects may also occur in vitamin A deficiency, where some patients report seeing “white as green” as a result of loss of S-cone function, and in diabetes. The recovery of S-cone function following vitamin A supplementation can be monitored with S-cone ERGs [10].

Patient population

Patients of all ages, able to tolerate Ganzfeld stimulation, referred for investigation of possible retinal dysfunction, retinal dystrophy, generalized cone or rod system dysfunction or patients with photophobia and/or nystagmus may benefit from the S-cone ERG, performed in addition to the ISCEV standard ERG.

Technical issues

S-cone ERG recording is performed following the specifications of the current ISCEV standard for full-field ERG [1]. Recordings of the S-cone ERG can be embedded within the standard protocol [1] or conducted as a separate protocol. Additional factors are outlined below:

  1. a.

    Spectral characteristics and luminance of the background

The photopic background for the recording of the S-cone ERG must be of spectral composition and strength that will achieve two goals: (1) saturate the rod system and (2) minimize the contributions of the L/M-cones systems. The initial studies used a white background, but later studies and the developments of new electrophysiology of vision systems indicated that bright LEDs with spectral peaks between 570 and 620 nm (yellow, amber, orange) were more suitable for the purpose of obtaining an S-cone ERG (Table 1). A high background luminance of appropriate wavelength may optimize isolation of S-cone-mediated responses, but patient comfort, tolerance and the possibility of photophobia must also be considered.

Table 1 Stimulus and background conditions for recording S-cone ERG
  1. b.

    Spectral characteristics of the test flash

Short-wavelength (blue) stimuli may be generated using Xenon light in combination with a suitable optical filter (Kodak #98, having a peak transmission at 450 nm) or by using a light emitting diode (LED) with a peak output of short wavelength, in the region of 440–470 nm (Table 1). Wavelengths longer than 470 nm typically generate ERGs that are dominated by the L/M-cones, and the contribution of the S-cones to the ERG may not be detectable. Light sources with wavelength shorter than 440 nm may not have sufficient strength to elicit a reliable S-cone ERG because the S-cone ERG action spectrum peaks at 450 nm [3].

  1. c.

    Strength of the test flash

Laboratories that use backgrounds of 300 cd m−2 have used flashes of low strength, 0.03–0.05 cd s m−2, while laboratories using bright backgrounds of about 500 cd m−2 use stronger flashes, 0.1–0.5 cd s m−2, in order to elicit robust S-cone mediated responses (Table 1). Weak flashes may be more selective but elicit responses of relatively low amplitude (Fig. 1), whereas stronger stimuli may be difficult for some patients to tolerate.

  1. d.

    Units of flash strength and luminance

It is acknowledged that the strength of the colored flash requires consideration of the spectral sensitivity of the eye and is accurately described by radiant energy (Watts/steradian/m2/nm), but for uniformity of clinical use and consistency with other brief flash stimuli, strength is defined in this protocol in units of cd s m−2. For stimulus durations > 10 ms, stimulus strength is given in units of luminance (cd m−2).


Calibration in accordance with the ISCEV standard ERG is recommended [1]. A spectral photometer is required to determine the spectral characteristics of the short-wavelength flash. Care should be taken to measure a range of flash luminance levels as some Ganzfeld stimulators use different combinations and banks of LEDs for different luminance ranges, and these may have different wavelength specifications.

Protocol specifications

Patient preparation follows that for the current ISCEV standard ERG [1]. For routine applications, the S-cone ERG protocol may be added to the ISCEV standard protocol after the other LA ERGs. Additional specifications are outlined in Table 1.

  1. a.

    Spectral characteristics and luminance of the background.

The background peak wavelength is between 570 and 620 nm (yellow, amber, orange). The background luminance is 300 cd m−2. Recordings obtained using higher luminance backgrounds may be added (see section c).

  1. b.

    Spectral characteristics of the test flash

For routine clinical diagnostics, LEDs with peak wavelength of between 450 nm and 470 nm are specified. If Xenon flashes and filters are used, a blue filter (Kodak #98 with peak transmission at 450 nm) is used. The method of stimulation (LED or optical filter) and the peak wavelength and bandwidth at half-height of the stimulus should be stated.

  1. c.

    Stimulus strength

The minimum S-cone ERG protocol includes a stimulus strength of 0.05 cd s m−2 delivered on a background luminance of 300 cd m−2. This does not preclude the recording of additional S-cone ERGs or an S-cone ERG stimulus–response series, e.g., stimuli ranging from approximately 0.025 to 0.2 cd s m−2 (see Fig. 1). Additional stronger flashes (e.g., 0.1–0.5 cd s m−2) on a brighter background luminance (e.g., 500–560 cd m−2) can be used but may be difficult for some patients to tolerate.

  1. d.

    Flash duration and frequency of presentation

Flash duration should be equal or less than 5 ms. The presentation rate is 2 Hz (inter-stimulus interval 0.5 s) as for the ISCEV standard LA3ERG.

  1. e.

    Signal averaging

Small responses to dim flashes or abnormally reduced S-cone ERG components may require signal averaging of up to several hundred recordings.

  1. f.

    Duration of light adaptation

When the S-cone ERG protocol is embedded in the full-field ERG protocol and conducted as a continuation of the light-adapted ERG recording, then the 10 min adaptation to the light-adapted background (white light 30 cd m−2) is sufficient before starting to record the S-cone ERG. However, if the S-cones ERG protocol is used as a stand-alone protocol, light adaptation to the background should proceed for 10 min before starting to record the S-cone ERG.

Response evaluation

The waveform is evaluated as having a single or double peak. If there are two peaks, the peak time is measured from stimulus onset to the 2nd (S-cone-mediated) peak. Amplitude is measured from baseline to the peak or from the trough that follows the 1st peak to the peak of the following positive wave depending upon response waveform (Fig. 2).

Fig. 2

Measuring the amplitude of the S-cone ERG in a response to a dim stimulus that contains only one peak and in a response to a stimulus of moderate strength that contains 2 peaks, the first is the L/M-cone ERG and the second the S-cone ERG. Amplitude is measured from the trough preceding the S-cone ERG peak (lower and upper horizontal dashed blue lines, respectively)


Reporting the S-cone ERG should follow the recommendations of the ISCEV standard ERG [1]. The spectral characteristics of the stimulus and background should be specified. The strength and duration of the light stimulus and the luminance of the background should be stated. The presence or absence of a 2-peak ERG pattern should be acknowledged, and amplitude and peak time of the 2nd peak should be reported as S-cone ERG along with age-appropriate laboratory reference data. If not already documented as part of the ISCEV standard ERG, pupil diameters following mydriasis should be documented. It is acknowledged that for routine diagnostic reporting it may be enough to describe the S-cone ERG qualitatively.


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Correspondence to Ido Perlman.

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Appendix: Justification for the protocol details and excluded methods

Appendix: Justification for the protocol details and excluded methods

A literature review was performed with PubMed search to find the publications that reported recordings of S-cone ERG using the following keywords: (S-cone or blue-cone) and (electroretinogram or ERG). Only the reports of S-cone ERG using a blue flash on strong adapting background illumination to suppress M- and L-cone activities were extracted. Animal studies were excluded, as well as studies using focal types of stimulation. Reports of S-cone ERG recording using silent substitution methods were also excluded. Studies from the year 1970 to 2018 were reviewed, and those with specified stimulus parameters are summarized in Table 1.

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Perlman, I., Kondo, M., Chelva, E. et al. ISCEV extended protocol for the S-cone ERG. Doc Ophthalmol 140, 95–101 (2020).

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  • Clinical standards
  • Electroretinogram (ERG)
  • Full-field ERG
  • International Society for Clinical Electrophysiology of Vision (ISCEV)
  • S-cone ERG
  • Short-wavelength-sensitive cone ERG
  • Retinopathy
  • Retinal dystrophy