Documenta Ophthalmologica

, Volume 109, Issue 1, pp 73–86 | Cite as

Interspecies and gender differences in multifocal electroretinograms of cynomolgus and rhesus macaques

  • Charlene B. Y. Kim
  • James N. Ver Hoeve
  • Paul L. Kaufman
  • Michael T. Nork
Article

Abstract

The purpose of this study is to ascertain whether inherent differences exist in the retinal function of two macaque species that are commonly used in experimental glaucoma investigations. We quantified and compared multifocal electroretinograms (mfERGs) from ocularly normal cynomolgus (n = 6) and rhesus (n = 8) monkeys. The stimulus array consisted of 103 equal-sized hexagonal elements, which subtended ±44° about the central visual axis. Mean luminance of the display was 100 cd/m2. The first-order kernel (K1) and second-order (first slice) kernel (K2) of the mfERG were averaged into 4 rings radiating from the foveal element, and represented the central 56° of visual field. Fifteen and 30-element segments were used for K1 and K2 quadrant and hemiretinal response determinations, respectively. Response measures for the rings, quadrants, and hemiretinae included K1 amplitude and implicit time, and K1 and K2 oscillatory potentials (OPs) and response amplitude root mean square (RMS). Species, gender, and retinotopic differences were assessed with repeated measures analysis of variance (split plot design). K1 amplitudes of the N1 waves, K1 and K2 OPs and K2 amplitude RMS for the ring, quadrant, and hemiretinal mfERG waveforms were larger in rhesus than in cynomolgus monkeys. Rhesus males (as compared to rhesus females) and cynomolgus females (as compared to cynomolgus males) exhibited larger amplitudes and less delayed implicit times in the central retina. These results demonstrate that species-specific differences in retinal function are evident in cynomolgus and rhesus monkeys. There also were gender-associated differences that varied across species. Thus, investigators should exercise caution when data from species or gender are combined.

cynomolgus macaques gender multifocal electroretinogram oscillatory potentials rhesus macaques root mean square 

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References

  1. 1.
    Spear PD. Neural bases of visual deficits during aging. Vision Res 1993; 33: 2589–2609.PubMedGoogle Scholar
  2. 2.
    Hood DC, Frishman LJ, Viswanathan S, Robson JG, Ahmed J. Evidence for a ganglion cell contribution to the primate electroretinogram (ERG): Effects of TTX on the multifocal ERG in macaque. Vis Neurosci 1999; 16: 411–6.PubMedGoogle Scholar
  3. 3.
    Frishman LJ, Saszik S, Harwerth RS et al. Effects of experimental glaucoma in macaques on the multifocal ERG Multifocal ERG in laser-induced glaucoma. Doc Ophthalmol 2000; 100: 231–51.Google Scholar
  4. 4.
    Hood DC, Frishman LJ, Saszik S, Viswanathan S. Reti-nal origins of the primate multifocal ERG: implications for the human response. Invest Ophthalmol Vis Sci 2002; 43: 1673–85.PubMedGoogle Scholar
  5. 5.
    Hare WA, Ton H, Ruiz G, Feldmann B, Wijono M, WoldeMussie E. Characterization of retinal injury using ERG measures obtained with both conventional and mul-tifocal methods in chronic ocular hypertensive primates. Invest Ophthalmol Vis Sci 2001; 42: 127–36.PubMedGoogle Scholar
  6. 6.
    Raz D, Perlman I, Percicot CL, Lambrou GN, Ofri R. Functional damage to inner and outer retinal cells in experimental glaucoma. Invest Ophthalmol Vis Sci 2003; 44: 3675–84.PubMedGoogle Scholar
  7. 7.
    Cowey A, Ellis CM. Visual acuity of rhesus and squirrel monkeys. J Comp Physiol Psychol 1967; 64: 80–4.PubMedGoogle Scholar
  8. 8.
    Borwein B, Borwein D, Medeiros J, McGowan JW. The ultrastructure of monkey foveal photoreceptors, with special reference to the structure, shape, size, and spacing of the foveal cones. Am J Anat 1980; 159: 125–46.PubMedGoogle Scholar
  9. 9.
    Perry V, Cowey A. The ganglion cell and cone distribu-tions in the monkey 's retina: implications for central magnification factors. Vision Res 1985; 25: 1795–810.PubMedGoogle Scholar
  10. 10.
    Kaufman P, Calkins B, Erickson K. Ocular biometry of the cynomolgus monkey. Curr Eye Res 1981; 1: 307–9.PubMedGoogle Scholar
  11. 11.
    DeRousseau CJ, Bito LZ. Intraocular pressure of rhesus monkeys (Macaca mulatta )II. Juvenile ocular hyperten-sion and its apparent relationship to ocular growth. Exper Eye Res 1981; 32: 407–17.Google Scholar
  12. 12.
    Watanabe M, Rodieck RW. Parasol and midget gan-glion cells of the primate retina. J Comp Neurol 1989; 289: 434–54.PubMedGoogle Scholar
  13. 13.
    Sutter EE. The interpretation of multifocal binary ker-nels. Doc Ophthalmol 2000; 100: 49–75.Google Scholar
  14. 14.
    MacNeil MA, Heussy JK, Dacheux RF, Raviola E, Masland RH. The shapes and numbers of amacrine cells: matching of photo lled with Golgi-stained cells in the rabbit retina and comparison with other mammalian spe-cies. J Comp Neurol 1999; 413: 305–26.PubMedGoogle Scholar
  15. 15.
    Rodieck RW. The primate retina. In: Steklis HD, Erwin J, eds. Comparative primate biology. Neurosciences Vol. 4 New York: Alan R. Liss, 1988: 203–78.Google Scholar
  16. 16.
    Hood DC, Greenstein V, Frishman L et al. Identifying inner retinal contributions to the human multifocal ERG. Vision Res 1999; 39: 2285–91.PubMedGoogle Scholar
  17. 17.
    Hood DC, Bearse MA, Sutter EE, Viswanathan S, Frishman LJ. The optic nerve head component of the monkey 's (Macaca mulatta )multifocal electroretinogram (mERG). Vision Res 2001; 41: 2029–41.PubMedGoogle Scholar
  18. 18.
    Birch D, Anderson J. Standardized full-eld electroreti-nography. Normal values and their variation with age. Arch Ophthalmol 1992; 110: 1571–76.PubMedGoogle Scholar
  19. 19.
    Fortman JD, Hewett TA, Bennett BT. The laboratory nonhuman primate. Boca Raton, FL: CRC Press, 2002: 266.Google Scholar
  20. 20.
    Wikler KC, Williams RW, Rakic P. Photoreceptor mosaic: number and distribution of rods and cones in the rhesus monkey retina. J Comp Neurol 1990; 297: 499–508.PubMedGoogle Scholar
  21. 21.
    Kim CBY, Tom BW, Spear PD. Effects of aging on the densities, numbers, and sizes of retinal ganglion cells in rhesus monkey. Neurobiol Aging 1996; 17: 431–8.PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Charlene B. Y. Kim
    • 1
  • James N. Ver Hoeve
    • 1
  • Paul L. Kaufman
    • 1
  • Michael T. Nork
    • 1
  1. 1.Department of Ophthalmology and Visual SciencesUniversity of Wisconsin Medical SchoolMadison, WI 53792-3284USA (

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