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High-Fat Diet Causes Constipation in Mice via Decreasing Colonic Mucus

  • Rieko Mukai
  • Osamu HandaEmail author
  • Yuji Naito
  • Shun Takayama
  • Yosuke Suyama
  • Chihiro Ushiroda
  • Atsushi Majima
  • Yasuko Hirai
  • Katsura Mizushima
  • Tetsuya Okayama
  • Kazuhiro Katada
  • Kazuhiro Kamada
  • Kazuhiko Uchiyama
  • Takeshi Ishikawa
  • Tomohisa Takagi
  • Yoshito Itoh
Original Article
  • 32 Downloads

Abstract

Background

Constipation is one of the most common gastrointestinal complaints. Although the causes of constipation are varied, dietary habits have a significant influence. Excessive fat intake is suggested as one of the main causes of constipation; however, the exact mechanism is unknown.

Aims

To investigate whether a high-fat diet (HFD) causes constipation in mice and to clarify the underlying mechanism, focusing on the amount of colonic mucus.

Methods

Six-week-old male C57BL/6 mice were randomly divided into two groups: mice fed with HFD and those with normal chow diet (NCD). Fecal weight, water content, total gastrointestinal transit time, and colon transit time were measured to determine whether the mice were constipated. The colonic mucus was evaluated by immunostaining and quantified by spectrometry. Malondialdehyde (MDA) was measured using the thiobarbituric acid (TBA) test as a marker for oxidative stress.

Results

Compared to the NCD group, the weight of feces was less in the HFD group. In the functional experiment, the total gastrointestinal transit time and colon transit time were longer in the HFD group. Furthermore, HFD significantly reduced the amount of colonic mucus. In addition, the reduction in colonic mucus caused by surfactant resulted in constipation in the NCD group.

Conclusions

HFD causes constipation with delayed colon transit time possibly via the reduction in colonic mucus in mice.

Keywords

High-fat diet Constipation Total gastrointestinal transit time Colon transit time Mucus 

Notes

Acknowledgment

We thank Professor Akiko Shiotani (Department of Gastroenterology, Kawasaki Medical School) for her data analysis support and assistance in the preparation of this manuscript.

Funding

This work was supported by Grants-in-Aid for Scientific Research (B) (KAKENHI) to Y.N. (No.16675576) from the Japan Society for the Promotion of Science, and by Grant of Industry-Academia-Government Collaboration of “Field for Knowledge Integration and Innovation” (FKII) to Y.N. (No. 16824414) from the Ministry of Agriculture, Forestry and Fisheries of Japan.

Compliance with Ethical Standards

Conflicts of interest

Yuji Naito received a scholarship fund from EA Pharma. Co. Ltd. and collaboration research fund from Fujifilm Medical Co., Ltd. and has been paid lecture fees by Janssen Pharma K.K., Mylan EPD Co., Takeda Pharma. Co. Ltd., Mochida Pharma. Co. Ltd., EA Pharma. Co. Ltd., Otsuka Pharma. Co. Ltd., Astellas Pharma. Co. Ltd., and Miyarisan Pharma. Co. Ltd. The research was partly funded by these funds. Neither the funding agency nor any outside organization has participated in study design or has any competing of interest. These companies had final approval of the manuscript.

Ethics approval and consent to participate

All experiments were carried out in accordance with the National Institutes of Health (NIH) guidelines for the use of experimental animals, and the protocols were approved (Approval No. M29-535) by the Animal Care Committee of the Kyoto Prefectural University of Medicine (Kyoto, Japan).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Rieko Mukai
    • 1
  • Osamu Handa
    • 1
    • 2
    Email author
  • Yuji Naito
    • 1
  • Shun Takayama
    • 1
  • Yosuke Suyama
    • 1
  • Chihiro Ushiroda
    • 1
  • Atsushi Majima
    • 1
  • Yasuko Hirai
    • 1
  • Katsura Mizushima
    • 1
  • Tetsuya Okayama
    • 1
  • Kazuhiro Katada
    • 1
  • Kazuhiro Kamada
    • 1
  • Kazuhiko Uchiyama
    • 1
  • Takeshi Ishikawa
    • 1
  • Tomohisa Takagi
    • 1
  • Yoshito Itoh
    • 1
  1. 1.Department of Molecular Gastroenterology and HepatologyKyoto Prefectural University of MedicineKyotoJapan
  2. 2.Division of Gastroenterology, Department of Internal MedicineKawasaki Medical SchoolOkayamaJapan

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