Digestive Diseases and Sciences

, Volume 61, Issue 10, pp 2868–2877 | Cite as

Helicobacter pylori Strains from Duodenal Ulcer Patients Exhibit Mixed babA/B Genotypes with Low Levels of BabA Adhesin and Lewis b Binding

  • Samaneh Saberi
  • Alexej Schmidt
  • Sana Eybpoosh
  • Maryam Esmaili
  • Yeganeh Talebkhan
  • Nazanin Mohajerani
  • Akbar Oghalaie
  • Mahmoud Eshagh Hosseini
  • Mohammad Ali Mohagheghi
  • Jeanna Bugaytova
  • Thomas Borén
  • Marjan Mohammadi
Original Article

Abstract

Background

BabA is a Helicobacter pylori cell surface adhesin, which binds to the ABO/Leb histo-blood group antigens (Leb) and serves as a virulence factor.

Methods

H. pylori single colonies were isolated from 156 [non-ulcer dyspepsia (NUD) = 97, duodenal ulcer (DU) = 34, gastric cancer (GC) = 25)] patients. babA and babB genes were evaluated by gene/locus-specific PCR. BabA protein expression and Leb binding activity were determined by immunoblotting and ELISA, respectively.

Results

The combined categorization of H. pylori strains based on high, low or no levels of BabA expression and Leb binding, produced 4 groups: (I) BabA-high/Leb-high (36 %), (II) BabA-low/Leb-low (26 %), (III) BabA-neg/Leb-low (30 %) and (IV) BabA-neg/Leb-neg (8 %) strains. The majority (63 %) of the BabA-low/Leb-low strains exhibited mixed babA/B genotypes as compared to merely 18 % of the BabA-high/Leb-high, 15 % of the BabA-neg/Leb-neg and 11 % of the BabA-neg/Leb-low (P = 0.0001) strains. In contrast to NUD strains, the great majority (70 %) of DU strains were BabA-low/Leb-low (11 %, P = 0.0001), which compared to NUD strains, enhanced the risk of DU by 18.8-fold. In parallel, infection with babA/B mixed genotype strains amplified the risk of DU by 3.6-fold (vs. babA-positive: P = 0.01) to 6.9-fold (vs. babA-negative: P = 0.007).

Conclusions

Here, we show higher prevalence of mixed babA/B genotypes among BabA-low/Leb-low clinical strains. Recombination of babA and babB genes across their loci may yield lower BabA expression and lower Leb binding activity. We conclude that H. pylori strains with lower Leb binding activity are better adapted for colonization of the gastric metaplastic patches in the duodenum and enhance the risk of duodenal ulcers.

Keywords

Helicobacter pylori Duodenal ulcer Mixed babA/B genotypes BabA Adhesin 

Notes

Acknowledgments

This study was generously supported by a technical assistance grant (IRN-072) to M.M., which was co-funded by the Islamic Development Bank, Saudi Arabia, and Pasteur Institute of Iran. T.B. is the founder of Helicure and a member of its scientific advisory board. He is supported by grants from Vetenskapsrådet/VR, Cancerfonden, the J.C. Kempe and Seth M. Kempe Memorial Foundation and the Knut and Alice Wallenberg Foundation (2012.0090).

Compliance with ethical standards

Conflicts of interest

The authors disclose no conflicts of interest.

Supplementary material

10620_2016_4217_MOESM1_ESM.docx (184 kb)
Supplementary material 1 (DOCX 185 kb)

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Samaneh Saberi
    • 1
  • Alexej Schmidt
    • 2
  • Sana Eybpoosh
    • 3
  • Maryam Esmaili
    • 1
  • Yeganeh Talebkhan
    • 1
  • Nazanin Mohajerani
    • 1
  • Akbar Oghalaie
    • 1
  • Mahmoud Eshagh Hosseini
    • 4
  • Mohammad Ali Mohagheghi
    • 5
  • Jeanna Bugaytova
    • 6
  • Thomas Borén
    • 6
  • Marjan Mohammadi
    • 1
  1. 1.HPGC Group, Department of Medical Biotechnology, Biotechnology Research CenterPasteur Institute of IranTehranIran
  2. 2.Department of Medical Biosciences and PathologyUmeå UniversityUmeåSweden
  3. 3.Research Center for Modeling in Health, Institute for Future Studies in HealthKerman University of Medical SciencesKermanIran
  4. 4.Department of Gastroenterology, Amiralam HospitalTehran University of Medical SciencesTehranIran
  5. 5.Cancer Research CenterTehran University of Medical SciencesTehranIran
  6. 6.Department of Medical Biochemistry and BiophysicsUmeå UniversityUmeåSweden

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