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Digestive Diseases and Sciences

, Volume 61, Issue 1, pp 208–214 | Cite as

Correlation Between Capillary and Venous Blood Glucose in the Lactose Tolerance Test

  • José Luis Domínguez JiménezEmail author
  • Antonio Fernández Suárez
Original Article

Abstract

Background

The lactose tolerance test is a classic method for the study of lactose malabsorption. However, the methodology used has not been standardized, and this leads to differences in results.

Aim

The aim of this report was to analyze whether capillary blood glucose measurement is in agreement with venous blood glucose when performing lactose tolerance test.

Methods

This is a prospective study of consecutive patients with suspected lactose malabsorption who had lactose tolerance test performed in venous and capillary blood simultaneously, using a load of 50 g lactose. Agreement was measured using the concordance correlation coefficient of Lin (95 % CI) and Bland–Altman plots. The degree of agreement was measured using the Kappa index. A value of p < 0.05 was considered statistically significant.

Results

Eighty-four patients (68 % women) were included. The concordance correlation coefficient showed very poor agreement between the two techniques: 0.68 (0.58–0.77), 0.72 (0.62–0.8), and 0.77 (0.69–0.83) for baseline, 30, and 60 min, respectively. The Bland–Altman plots showed that capillary blood glucose measurements result in higher levels than venous blood glucose measurements, with mean differences of 0.39, 0.77, and 1.1 mmol/L at baseline, 30, and 60 min, respectively. The degree of agreement was low, with a Kappa index of 0.59 (p < 0.001).

Conclusions

The test measured in venous blood is not in agreement with the measurement obtained from capillary blood. It is likely that the diagnostic accuracy attributed without distinction to lactose tolerance test in different studies for lactose malabsorption is incorrect, making it necessary to specify the analysis method.

Keywords

Lactose tolerance test Lactose intolerance diagnosis Lactose malabsorption Glycemic variability 

Abbreviations

LI

Lactose intolerance

LM

Lactose malabsorption

LHBT

Lactose hydrogen breath test

LTT

Lactose tolerance test

POCT

Point-of-care testing

LFD

Lactose-free diet

DM

Diabetes mellitus

rc

Concordance correlation coefficient of Lin

Notes

Acknowledgments

We appreciate the tireless work of the nursing and medical consultation supporting staff, without which it would have been impossible to conduct this study.

Compliance with ethical standards

Conflict of interest

None.

Informed consent

The protocol was approved by the institutional ethics committee, and informed consent was obtained from all patients.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • José Luis Domínguez Jiménez
    • 1
    Email author
  • Antonio Fernández Suárez
    • 2
  1. 1.Department of Gastroenterology and Hepatology, Alto Guadalquivir Healthcare AgencyAlto Guadalquivir HospitalAndújarSpain
  2. 2.Department of Biotechnology, Alto Guadalquivir Healthcare AgencyAlto Guadalquivir HospitalAndújarSpain

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