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Digestive Diseases and Sciences

, Volume 60, Issue 6, pp 1595–1602 | Cite as

Nesfatin-1 Suppresses Gastric Contractions and Inhibits Interdigestive Migrating Contractions in Conscious Dogs

  • Akira WatanabeEmail author
  • Erito Mochiki
  • Akiharu Kimura
  • Norimichi Kogure
  • Mitsuhiro Yanai
  • Atsushi Ogawa
  • Yoshitaka Toyomasu
  • Kyoichi Ogata
  • Tetsuro Ohno
  • Hideki Suzuki
  • Hiroyuki Kuwano
Original Article

Abstract

Background

Nesfatin-1 is a novel 82-amino acid anorectic peptide. Acute injection of nesfatin-1 into the third brain ventricle reduces food consumption during the dark phase in rats. Nesfatin-1 is also expressed in gastric X/A-like cells in the peripheral tissues. Nesfatin-1 has been reported to reduce gastric and duodenal motility and to delay gastric emptying.

Aim

In the present study, we investigated the effects of nesfatin-1 on gastrointestinal motility in conscious dogs.

Methods

Force transducers were implanted onto the serosal surfaces of the gastric bodies, gastric antra, duodena, and jejuna of healthy beagle dogs, and gastrointestinal motility was monitored. We evaluated the effects of nesfatin-1 on gastrointestinal motility and on the circulating levels of nesfatin-1 in the fasted and fed states.

Results

The intravenous administration of nesfatin-1 reduced gastric contractions and inhibited cyclical interdigestive migrating contractions in the fasted state. In the fasted state, circulating levels of nesfatin-1 tended to increase during late phase I. In addition, the kinetics of the circulating levels of nesfatin-1 were opposite to those of ghrelin during the fasted state.

Conclusions

Nesfatin-1 regulates gastrointestinal motility, and, in particular, it inhibits gastric contractions in the fasted state. Interdigestive migrating contractions may be regulated by interactions between nesfatin-1, ghrelin, and motilin.

Keywords

Nesfatin-1 Ghrelin Motilin Gastrointestinal motility Interdigestive migrating contractions 

Abbreviations

IMCs

Interdigestive migrating contractions

MI

Motility index

NUCB2

Nucleobindin-2

Notes

Acknowledgments

We thank Ms. Yukie Saito, Ms. Tomoko Yano, Ms. Tomoko Ubukata, Ms. Yuka Matsui, Ms. Ayaka Ishida, and Ayaka Ishikubo for their excellent assistance.

Conflict of interest

None.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Akira Watanabe
    • 1
    • 3
    Email author
  • Erito Mochiki
    • 2
  • Akiharu Kimura
    • 1
  • Norimichi Kogure
    • 1
  • Mitsuhiro Yanai
    • 1
  • Atsushi Ogawa
    • 1
  • Yoshitaka Toyomasu
    • 1
  • Kyoichi Ogata
    • 1
  • Tetsuro Ohno
    • 1
  • Hideki Suzuki
    • 1
  • Hiroyuki Kuwano
    • 1
  1. 1.Department of General Surgical ScienceGunma University Graduate School of MedicineMaebashiJapan
  2. 2.Department of Digestive Tract and General Surgery, Saitama Medical CenterSaitama Medical UniversityKawagoeJapan
  3. 3.Department of General Surgical Science (Surgery I)Gunma University Graduate School of MedicineMaebashiJapan

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